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. 1970 Jun;18(6):799–806.

The demonstration of immunoglobulins in porcine intestinal tissue by immunofluorescences with observations on the effect of fixation

W D Allen, P Porter
PMCID: PMC1455731  PMID: 4193665

Abstract

The localization of immunoglobulins IgA, IgG and IgM in porcine intestinal tissue by immunofluorescence is described and the influence of three fixatives, ethanol, methanol and acetone on the findings compared. Methanol was found to be the only fixative to give consistent results for IgM; it was also the reagent of choice for the localization of IgA in cells of the lamina propria. However ethanol and acetone were preferred for the demonstration of secretory IgA. Extravascular IgG was shown to be still soluble after fixation and capable of being removed from the tissue. The demonstration of IgM in intestinal crypt epithelium is reported and its possible role as a secretory antibody acting in concert with IgA is discussed.

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Selected References

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  1. AUGUSTIN R., HAYWARD B. J. Human reagins to grass pollens and moulds: their purification and physico-chemical characterization. Immunology. 1960 Jan;3:45–73. [PMC free article] [PubMed] [Google Scholar]
  2. CARBONARA A. O., RODHAIN J. A., HEREMANS J. F. Localization of gama1A-globulin (beta2A-globulin) in tissue cells. Nature. 1963 Jun 8;198:999–1000. doi: 10.1038/198999a0. [DOI] [PubMed] [Google Scholar]
  3. CRABBE P. A., CARBONARA A. O., HEREMANS J. F. THE NORMAL HUMAN INTESTINAL MUCOSA AS A MAJOR SOURCE OF PLASMA CELLS CONTAINING GAMMA-A-IMMUNOGLOBULIN. Lab Invest. 1965 Mar;14:235–248. [PubMed] [Google Scholar]
  4. Eidelman S., Davis S. D. Immunoglobulin content of intestinal mucosal plasma-cells in ataxia telangiectasia. Lancet. 1968 Apr 27;1(7548):884–886. doi: 10.1016/s0140-6736(68)90238-9. [DOI] [PubMed] [Google Scholar]
  5. GOLDSTEIN G., SLIZYS I. S., CHASE M. W. Studies on fluorescent antibody staining. I. Non-specific fluorescence with fluorescein-coupled sheep anti-rabbit globulins. J Exp Med. 1961 Jul 1;114:89–110. doi: 10.1084/jem.114.1.89. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gelzayd E. A., Kraft S. C., Kirsner J. B. Distribution of immunoglobulins in human rectal mucosa. I. Normal control subjects. Gastroenterology. 1968 Mar;54(3):334–340. [PubMed] [Google Scholar]
  7. MAYERSBACH H. Unspecific interactions between serum and tissue sections in the fluorescent-antibody technic for tracing antigens in tissues. J Histochem Cytochem. 1959 Nov;7:427–427. doi: 10.1177/7.6.427. [DOI] [PubMed] [Google Scholar]
  8. Mayersbach H. V. Principles and limitations of immunohistochemical methods. J R Microsc Soc. 1967;87(2):295–308. [PubMed] [Google Scholar]
  9. Paronetto F., Koffler D. Immunofluorescent localization of immunoglobulins, complement, and fibrinogen in human diseases. I. Systemic lupus erythematosus. J Clin Invest. 1965 Oct;44(10):1657–1664. doi: 10.1172/JCI105272. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Porter P., Noakes D. E., Allen W. D. Secretory IgA and antibodies to Escherichia coli in porcine colostrum and milk and their significance in the alimentary tract of the young pig. Immunology. 1970 Feb;18(2):245–257. [PMC free article] [PubMed] [Google Scholar]
  11. Porter P. Porcine colostral IgA and IgM antibodies to Escherichia coli and their intestinal absorption by the neonatal piglet. Immunology. 1969 Oct;17(4):617–626. [PMC free article] [PubMed] [Google Scholar]
  12. RUBIN W., FAUCI A. S., MARVIN S. F., SLEISENGER M. H., JEFRIES G. H. IMMUNOFLUORESCENT STUDIES IN ADULT CELIAC DISEASE. J Clin Invest. 1965 Mar;44:475–485. doi: 10.1172/JCI105161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rossen R. D., Morgan C., Hsu K. C., Butler W. T., Rose H. M. Localization of 11 S external secretory IgA by immunofluorescence in tissues lining the oral and respiratory passages in man. J Immunol. 1968 Apr;100(4):706–717. [PubMed] [Google Scholar]
  14. Stobo J. D., Tomasi T. B. A Low Molecular Weight Immunoglobulin Antigenically Related to 19 S IgM. J Clin Invest. 1967 Aug;46(8):1329–1337. doi: 10.1172/JCI105625. [DOI] [PMC free article] [PubMed] [Google Scholar]

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