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. 1970 Jun;18(6):791–797.

Peritoneal macrophages in the immune response to SRBC in vitro

M Hoffmann
PMCID: PMC1455736  PMID: 4913802

Abstract

The role of macrophages in the immune response against SRBC in spleen cell suspension cultures obtained from mice was studied.

1. The presence of macrophages increases the number of PFC against SRBC in spleen cell cultures.

2. The ratio peritoneal exudate cells to spleen cells is very critical. Two × 105 peritoneal exudate cells preincubated with sheep red blood cells stimulate 1 × 107 mouse spleen cells to an optimal response. This reaction is suppressed when 2 × 106 peritoneal cells are used.

3. Spleen cell populations which had lost the capability of producing antibodies by elimination of cells adhering to glass yielded a strong immune response upon addition of 2 × 105 peritoneal exudate cells. The significance of macrophages for the induction of the immune response is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Argyris B. F. Role of macrophages in immunological maturation. J Exp Med. 1968 Sep 1;128(3):459–467. doi: 10.1084/jem.128.3.459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dresser D. W., Mitchison N. A. The mechanism of immunological paralysis. Adv Immunol. 1968;8:129–181. doi: 10.1016/s0065-2776(08)60466-6. [DOI] [PubMed] [Google Scholar]
  3. FREI P. C., BENACERRAF B., THORBECKE G. J. PHAGOCYTOSIS OF THE ANTIGEN, A CRUCIAL STEP IN THE INDUCTION OF THE PRIMARY RESPONSE. Proc Natl Acad Sci U S A. 1965 Jan;53:20–23. doi: 10.1073/pnas.53.1.20. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Harris G. Studies of the mechanism of antigen stimulation of DNA synthesis in rabbit spleen cultures. Immunology. 1965 Dec;9(6):529–541. [PMC free article] [PubMed] [Google Scholar]
  5. Miller J. F., Mitchell G. F. Cell to cell interaction in the immune response. I. Hemolysin-forming cells in neonatally thymectomized mice reconstituted with thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):801–820. doi: 10.1084/jem.128.4.801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):821–837. doi: 10.1084/jem.128.4.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mitchison N. A. The immunogenic capacity of antigen taken up by peritoneal exudate cells. Immunology. 1969 Jan;16(1):1–14. [PMC free article] [PubMed] [Google Scholar]
  9. Mosier D. E. A requirement for two cell types for antibody formation in vitro. Science. 1967 Dec 22;158(3808):1573–1575. doi: 10.1126/science.158.3808.1573. [DOI] [PubMed] [Google Scholar]
  10. Mosier D. E., Coppleson L. W. A THREE-CELL INTERACTION REQUIRED FOR THE INDUCTION OF THE PRIMARY IMMUNE RESPONSE in vitro. Proc Natl Acad Sci U S A. 1968 Oct;61(2):542–547. doi: 10.1073/pnas.61.2.542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PERKINS E. H., MAKINODAN T. THE SUPPRESSIVE ROLE OF MOUSE PERITONEAL PHAGOCYTES IN AGGLUTININ RESPONSE. J Immunol. 1965 May;94:765–777. [PubMed] [Google Scholar]
  12. Unanue E. R., Askonas B. A. The immune response of mice to antigen in macrophages. Immunology. 1968 Aug;15(2):287–296. [PMC free article] [PubMed] [Google Scholar]

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