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. 1996 Jan 1;24(1):119–120. doi: 10.1093/nar/24.1.119

Databases and software for the analysis of mutations in the human p53 gene, the human hprt gene and the lacZ gene in transgenic rodents.

N F Cariello 1, G R Douglas 1, T Soussi 1
PMCID: PMC145576  PMID: 8594557

Abstract

We have created databases and software applications for the analysis of DNA mutations in the human p53 gene, the human hprt gene and the rodent transgenic lacZ locus. The databases themselves are stand-alone dBase files and the software for analysis of the databases runs on IBM- compatible computers. The software created for these databases permits filtering, ordering, report generation and display of information in the database. In addition, a significant number of routines have been developed for the analysis of single base substitutions. One method of obtaining the databases and software is via the World Wide Web (WWW). Open home page http://sunsite.unc.edu/dnam/mainpage.ht ml with a WWW browser. Alternatively, the databases and programs are available via public ftp from anonymous@sunsite.unc.edu. There is no password required to enter the system. The databases and software are found in subdirectory pub/academic/biology/dna-mutations. Two other programs are available at the WWW site, a program for comparison of mutational spectra and a program for entry of mutational data into a relational database.

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Selected References

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  1. Albertini R. J., O'Neill J. P., Nicklas J. A., Heintz N. H., Kelleher P. C. Alterations of the hprt gene in human in vivo-derived 6-thioguanine-resistant T lymphocytes. Nature. 1985 Jul 25;316(6026):369–371. doi: 10.1038/316369a0. [DOI] [PubMed] [Google Scholar]
  2. Cariello N. F., Beroud C., Soussi T. Database and software for the analysis of mutations at the human p53 gene. Nucleic Acids Res. 1994 Sep;22(17):3549–3550. [PMC free article] [PubMed] [Google Scholar]
  3. Cariello N. F., Cui L., Beroud C., Soussi T. Database and software for the analysis of mutations in the human p53 gene. Cancer Res. 1994 Aug 15;54(16):4454–4460. [PubMed] [Google Scholar]
  4. Cariello N. F. Database and software for the analysis of mutations at the human hprt gene. Nucleic Acids Res. 1994 Sep;22(17):3547–3548. [PMC free article] [PubMed] [Google Scholar]
  5. Cariello N. F., Piegorsch W. W., Adams W. T., Skopek T. R. Computer program for the analysis of mutational spectra: application to p53 mutations. Carcinogenesis. 1994 Oct;15(10):2281–2285. doi: 10.1093/carcin/15.10.2281. [DOI] [PubMed] [Google Scholar]
  6. Cariello N. F. Software for the analysis of mutations at the human hprt gene. Mutat Res. 1994 Apr;312(2):173–185. doi: 10.1016/0165-1161(94)90023-x. [DOI] [PubMed] [Google Scholar]
  7. Caron de Fromentel C., Soussi T. TP53 tumor suppressor gene: a model for investigating human mutagenesis. Genes Chromosomes Cancer. 1992 Jan;4(1):1–15. doi: 10.1002/gcc.2870040102. [DOI] [PubMed] [Google Scholar]
  8. Douglas G. R., Jiao J., Gingerich J. D., Gossen J. A., Soper L. M. Temporal and molecular characteristics of mutations induced by ethylnitrosourea in germ cells isolated from seminiferous tubules and in spermatozoa of lacZ transgenic mice. Proc Natl Acad Sci U S A. 1995 Aug 1;92(16):7485–7489. doi: 10.1073/pnas.92.16.7485. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harbach P. R., Filipunas A. L., Wang Y., Aaron C. S. DNA sequence analysis of spontaneous and N-ethyl-N-nitrosourea-induced hprt mutations arising in vivo in cynomolgus monkey T-lymphocytes. Environ Mol Mutagen. 1992;20(2):96–105. doi: 10.1002/em.2850200205. [DOI] [PubMed] [Google Scholar]
  10. Hollstein M., Sidransky D., Vogelstein B., Harris C. C. p53 mutations in human cancers. Science. 1991 Jul 5;253(5015):49–53. doi: 10.1126/science.1905840. [DOI] [PubMed] [Google Scholar]
  11. Jones I. M., Burkhart-Schultz K., Crippen T. L. Cloned mouse lymphocytes permit analysis of somatic mutations that occur in vivo. Somat Cell Mol Genet. 1987 Jul;13(4):325–333. doi: 10.1007/BF01534926. [DOI] [PubMed] [Google Scholar]
  12. Provost G. S., Kretz P. L., Hamner R. T., Matthews C. D., Rogers B. J., Lundberg K. S., Dycaico M. J., Short J. M. Transgenic systems for in vivo mutation analysis. Mutat Res. 1993 Jul;288(1):133–149. doi: 10.1016/0027-5107(93)90215-2. [DOI] [PubMed] [Google Scholar]
  13. Turner D. R., Morley A. A., Haliandros M., Kutlaca R., Sanderson B. J. In vivo somatic mutations in human lymphocytes frequently result from major gene alterations. Nature. 1985 May 23;315(6017):343–345. doi: 10.1038/315343a0. [DOI] [PubMed] [Google Scholar]

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