Abstract
Synovitis was produced in guinea-pigs previously immunized with bovine γ-globulin and Freund's complete adjuvant, by injection of bovine γ-globulin and tuberculin PPD into the knee joints. In animals given prior intravenous injections of tritiated thymidine and colloidal carbon, up to 40 per cent of the local accumulations of mononuclear cells 48 hours after antigen challenge were tritium-labelled as seen by autoradiography. These inflammatory cells are considered to be of haematogenous origin. A smaller percentage of cells was carbon-labelled. The percentage of tritium-labelled cells decreased with duration of the granuloma following antigen challenge. In other animals, synovial inflammatory cells were labelled by intra-articular injection of tritiated thymidine at different times following the establishment of synovial inflammation by injection of antigen. The highest proportion of cells labelled was 17 per cent, but when label was administered as late as 60 days after the inflammatory stimulus, only about 3 per cent of cells were labelled. Grain counts of cells labelled by local tritium administration or by short-term incubation of slices in vitro showed approximately 30–100 grains per nucleus. Sequential study of locally labelled synovia showed that such high-grain cells were still present 12 days after tritium administration, suggesting that at least some of the mononuclear cells were relatively long-lived.
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- DUMONDE D. C., GLYNN L. E. The production of arthritis in rabbits by an immunological reaction to fibrin. Br J Exp Pathol. 1962 Aug;43:373–383. [PMC free article] [PubMed] [Google Scholar]
- KOSUNEN T. U., WAKSMAN B. H., FLAX M. H., TIHEN W. S. Radioautographic study of cellular mechanisms in delayed hypersensitivity. I. Delayed reactions to tuberculin and purified proteins in the rat and guinea-pig. Immunology. 1963 May;6:276–290. [PMC free article] [PubMed] [Google Scholar]
- Loewi G. Experimental immune inflammation in the synovial membrane. I. The immunological mechanism. Immunology. 1968 Sep;15(3):417–427. [PMC free article] [PubMed] [Google Scholar]
- Spector W. G., Lykke A. W. The cellular evolution of inflammatory granulomata. J Pathol Bacteriol. 1966 Jul;92(1):163–167. doi: 10.1002/path.1700920117. [DOI] [PubMed] [Google Scholar]
- Spector W. G., Walters M. N., Willoughby D. A. The origin of the mononuclear cells in inflammatory exudates induced by fibrinogen. J Pathol Bacteriol. 1965 Jul;90(1):181–192. doi: 10.1002/path.1700900119. [DOI] [PubMed] [Google Scholar]
- Spector W. G., Willoughby D. A. The origin of mononuclear cells in chronic inflammation and tuberculin reactions in the rat. J Pathol Bacteriol. 1968 Oct;96(2):389–399. doi: 10.1002/path.1700960217. [DOI] [PubMed] [Google Scholar]
- VOLKMAN A., GOWANS J. L. THE ORIGIN OF MACROPHAGES FROM BONE MARROW IN THE RAT. Br J Exp Pathol. 1965 Feb;46:62–70. [PMC free article] [PubMed] [Google Scholar]
- VOLKMAN A., GOWANS J. L. THE PRODUCTION OF MACROPHAGES IN THE RAT. Br J Exp Pathol. 1965 Feb;46:50–61. [PMC free article] [PubMed] [Google Scholar]
- Volkman A. The origin and turnover of mononuclear cells in peritoneal exudates in rats. J Exp Med. 1966 Aug 1;124(2):241–254. doi: 10.1084/jem.124.2.241. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Furth R., Cohn Z. A. The origin and kinetics of mononuclear phagocytes. J Exp Med. 1968 Sep 1;128(3):415–435. doi: 10.1084/jem.128.3.415. [DOI] [PMC free article] [PubMed] [Google Scholar]