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. 1969 Nov;17(5):685–694.

A quantitative antibody-binding method for the determination of specific antibody within different immunoglobulin classes

Application to four Ig classes in the mouse

D R Nash, J F Heremans
PMCID: PMC1455912  PMID: 4187521

Abstract

A quantitative method for determining specific antibody in the different classes of immunoglobulins is described. The technique is based on the binding of all antibody present in an immune serum with antigen, and the subsequent precipitation of the bound immunoglobulins with a heterologous antiserum specific for the antigen employed. The concentration of each immunoglobulin class is determined by means of single radial immunodiffusion, before and after removal of its antibody-active portion. The accuracy of the measurements is essentially that of the immunodiffusion technique, and the whole analysis, applied to four immunoglobulin classes, requires only 0.06 ml of serum.

The applicability of the technique has been tested using sera from mice immunized with ferritin by different routes. Antibody activity was predominant in the IgG1 and IgG2 immunoglobulin classes. However, measurable anti-ferritin activity in the IgM and IgA classes was also found in a limited number of sera.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barth W. F., McLaughlin C. L., Fahey J. L. The immunoglobulins of mice. VI. Response to immunization. J Immunol. 1965 Nov;95(5):781–790. [PubMed] [Google Scholar]
  2. Bazin H. Les immunoglobulines de la souris. II. Etude des anticorps synthétisés dans les différentes classes d'immunoglobulines en réponse à l'injection de deux antigènes protéiques solubles. Ann Inst Pasteur (Paris) 1967 Feb;112(2):162–172. [PubMed] [Google Scholar]
  3. FAHEY J. L., WUNDERLICH J., MISHELL R. THE IMMUNOGLOBULINS OF MICE. I. FOUR MAJOR CLASSES OF IMMUNOGLOBULINS: 7S GAMMA-2-, 7S GAMMA-1-, GAMMA-1A (BETA-2A)-, AND 18S GAMMA-1M-GLOBULINS. J Exp Med. 1964 Aug 1;120:223–242. doi: 10.1084/jem.120.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gleich G. J., Stankievic R. R. Measurement of penicilloyl antibodies by radioimmunoprecipitation. Immunochemistry. 1969 Jan;6(1):85–99. doi: 10.1016/0019-2791(69)90181-5. [DOI] [PubMed] [Google Scholar]
  5. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  6. SCHULTZE H. E. The synthesis of antibodies and proteins. Clin Chim Acta. 1959 Sep;4:610–626. doi: 10.1016/0009-8981(59)90002-6. [DOI] [PubMed] [Google Scholar]
  7. SKOM J. H., TALMAGE D. W. Nonprecipitating insulin antibodies. J Clin Invest. 1958 Jun;37(6):783–786. doi: 10.1172/JCI103664. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Vaerman J. P., Lebacq-Verheyden A. M., Scolari L., Heremans J. F. Further studies on single radial immunodiffusion. II. The reversed system: diffusion of antibodies in antigen-containing gels. Immunochemistry. 1969 Mar;6(2):287–293. doi: 10.1016/0019-2791(69)90165-7. [DOI] [PubMed] [Google Scholar]
  9. YAGI Y., MAIER P., PRESSMAN D., ARBESMAN C. E., REISMAN R. E. THE PRESENCE OF THE RAGWEED-BINDING ANTIBODIES IN THE BETA-2A, BETA-2M, AND GAMMA GLOBULINS OF THE SENSITIVE INDIVIDUALS. J Immunol. 1963 Jul;91:83–89. [PubMed] [Google Scholar]

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