Abstract
Lepisosteus osseus possesses high titres of natural agglutinins to human group A (HuA) erythrocytes and to Salmonella typhosa `O' (STO) antigens. Moderate natural haemagglutinin titres to sheep red blood cells (SRBC) were noted. Cold agglutinin (4°) titres exceeded agglutinin titres recorded at 25°.
Immunization with STO antigen resulted in a marked increase in corresponding anti-STO agglutinins, as well as increases in anti-HuA and anti-SRBC agglutinins. Animals immunized with SRBC antigen produced elevated anti-SRBC agglutinin titres, while immunization with HuA erythrocytes produced no changes in agglutinin levels.
Immunization with STO following previous injections with HuA resulted in increases of corresponding anti-STO agglutinins, as well as marked increases in anti-HuA agglutinins. Absorption studies indicated that anti-STO agglutinins were not cross reactive against HuA antigens. Natural and immune antibodies were all of the same macromolecular class. Rises in antibody titres were reflected in both cold and warm agglutinins. Animals maintained for long periods at low temperatures exhibited preferential ability for increased synthesis of cold agglutinins to HuA.
Natural haemolytic titres, as measured by an SRBC indicator system, were also elevated as compared to mammalian systems. These titres increased with stimulation with SRBC. Results suggest that the high levels of natural haemolytic and agglutinin activity in this species are of immunogenic origin.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Avtalion R. R. Temperature effect on antibody production and immunological memory, in carp (Cyprinus carpio) immunized against bovine serum albumin (BSA). Immunology. 1969 Dec;17(6):927–931. [PMC free article] [PubMed] [Google Scholar]
- Bradshaw C. M., Clem L. W., Sigel M. M. Immunologic and immunochemical studies on the gar, Lepisosteus platyrhincus. I. Immune responses and characterization of antibody. J Immunol. 1969 Sep;103(3):496–504. [PubMed] [Google Scholar]
- JOHNSON A. G., GAINES S., LANDY M. Studies on the O antigen of Salmonella typhosa. V. Enhancement of antibody response to protein antigens by the purified lipopolysaccharide. J Exp Med. 1956 Feb 1;103(2):225–246. doi: 10.1084/jem.103.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Legler D. W., Evans E. E. Comparative immunology. Hemolytic complement in amphibia. Proc Soc Exp Biol Med. 1966 Apr;121(4):1158–1162. doi: 10.3181/00379727-121-30994. [DOI] [PubMed] [Google Scholar]
- MAYER M. M., LEVINE L. Kinetic studies on immune hemolysis. IV. Rate determination of the Mg++ and terminal reaction steps. J Immunol. 1954 Jun;72(6):516–530. [PubMed] [Google Scholar]
- SPRINGER G. F., HORTON R. E., FORBES M. [Origin of anti-human blood group B agglutinins in white Leghorn chicks]. J Exp Med. 1959 Aug 1;110(2):221–244. doi: 10.1084/jem.110.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Springer G. F., Horton R. E. Blood group isoantibody stimulation in man by feeding blood group-active bacteria. J Clin Invest. 1969 Jul;48(7):1280–1291. doi: 10.1172/JCI106094. [DOI] [PMC free article] [PubMed] [Google Scholar]