Abstract
Immune haemolysis was determined by rectilinear attenuance at 625 mμ (l = 1.3 mm, 1.25 mm), and the data obtained treated by von Krogh's empirical equation. The C′H50/ml values obtained by the attenuance method had high correlation with those obtained by the conventional photometric method (541 mμ).
Automatic complement titration was attempted, as a part of the basic studies on quantitative complement fixation tests.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- SHIBATA K. Spectrophotometry of opaque biological materials--reflection methods. Methods Biochem Anal. 1962;9:217–234. doi: 10.1002/9780470110256.ch6. [DOI] [PubMed] [Google Scholar]
- VARGUES R. RECHERCHES SUR LA CIN'ETIQUE DES R'EACTIONS DE FIXATION DU COMPL'EMENT. Ann Inst Pasteur (Paris) 1965 Feb;108:196–214. [PubMed] [Google Scholar]
- Vargues R., Gonthier F. Obtention automatique des courbes de titrage du complément. Etude critique de l'équation de von Krogh. Ann Inst Pasteur (Paris) 1965 Apr;108(4):526–533. [PubMed] [Google Scholar]
- Vargues R. The use of the autoanalyzer for the automatic titration of antigenic preparations by means of complement-fixation. Ann N Y Acad Sci. 1965 Nov 9;130(2):819–826. doi: 10.1111/j.1749-6632.1965.tb12625.x. [DOI] [PubMed] [Google Scholar]