Figure 3.

Comparison of the desat1 transcription pattern among sexes, genotypes, and species during development. Each transcript was detected by RT-PCR with the primers described in Figure 2. (A) The comparison was carried out between males and females at the following stages: third instar larva (L3), 24-hr-old adult (A1), and 5-day-old adult (A5). (B) Both sexes were pooled in first instar larva (L1). For D. melanogaster, the following genotypes were compared: wild-type Cs and homozygotes for the 1573-N2 rescued excision allele (N2), the 1573-1 mutant allele (1573-1), or the C′1 null allele (C′1). D. simulans is a wild-type strain. Five desat1 transcripts were detected: RA (1), RC (2), RE (3), RB (4), and RD (5). The desat2 transcript (6) and the Actin5C transcript (7) were used as internal markers. M1 and M2 are the molecular weight markers (MBI, Fermentas; 1 kb and 100 bp, respectively). The approximate size of the fragments is indicated on the right.