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Endogenous AWDKpn protein is present at higher levels than transgenic AWDKpn protein. Lysate from third instar larvae homozygous for endogenous awdKpn or homozygous for the transgenic P{awdKpn} on an awd null background were blotted with AWD antibody. A single band at 15 kDa was detected for both samples, representing AWD monomer. The endogenous AWDKpn protein is present at higher levels than the AWDKpn protein. The blot was normalized with β-tubulin to show equal loading of protein.
