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. 2006 Jan;172(1):99–112. doi: 10.1534/genetics.105.050427

Figure 8.

Figure 8.

SUM1-1-repressed genes in the absence of the HMR-I silencer. (A) MATα sir2Δ sum1Δ strains bearing a wild-type or synthetic HMR-E silencer containing one Gal4p-binding site (GEB) were generated. Strains were transformed with vector or plasmids expressing SUM1-1 (pRO711) or GBD-SUM1-1 (pRO707) protein chimeras. Patch-mating assays using a MATa tester lawn were performed to monitor silencing of the MATa1 reporter gene. (B) MATα sir2Δ sum1Δ strains bearing a synthetic HMR-E silencer without HMR-I were generated. One, three, or five GAL4-binding sites replaced the ARS element at HMR-E (ROY4039 HMRss1xGEBΔI, ROY4040 HMRss3xGEBΔI, and ROY4041 HMRss5xGEBΔI). Strains were transformed with vector or plasmids expressing GBD-SUM1-1 (pRO707) and HST1 (pRO713) and patch-mating assays using a MATa tester lawn were performed to monitor silencing of the MATa1 reporter gene.