TABLE 4.
No. of nonfunctional S-haplotypes in each Seedling
|
No. of seedlings analyzed
|
Phenotype of seedlings
|
|
---|---|---|---|
No. SI | No. SC | ||
1 | 17 | 17 | 0 |
2 | 17 | 3a | 14 |
3 | 37 | 0 | 37 |
4 | 21 | 0 | 21 |
Three progeny with S-genotype S4S6SaSd were determined to be SI, despite having two nonfunctional S-haplotypes. Partial S-RNase and SFB sequences from the Sa- and Sd- haplotypes are identical (N. R. Hauck, unpublished results), suggesting that the Sa and Sd represent different mutations of a common S-haplotype. We are currently testing the possibility that Sa has a functional S-RNase and a nonfunctional SFB, whereas Sd has a nonfunctional S-RNase and a functional SFB. In this case, S4S6SaSd individuals would be predicted to be SI under the one-allele-match model since SaSd pollen would be rejected due to a match between a functional Sa-RNase and SFBd.