Figure 5.

(A) Domain analysis of Zds1. Domain 1 (180–193) contains a sequence that is homologous to a region in S. cerevisiae Zds1p and Zds2p, domain 2 (227–238) contains a putative NLS, domain 3 (568–572) contains a region that is homologous to one in Zds2p, domain 4 (670–681) contains a Ser-rich sequence, and domain 5 (818–858) contains a putative functional domain. The M31 region is reported to localize to the nucleus (Sawin and Nurse 1996). (B) Western blot analysis of various Zds1–GFP fusion proteins. SP66 (wild-type) cells were transformed with the various plasmids shown in A and cultured to midlog phase. The proteins were then extracted. Arrowheads show various Zds1–GFP fusion proteins.