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Leg effects of Sb-sbd overexpression in pupae in the first (1st), second (2nd), and third (3rd) legs of adults from pupae carrying two copies of the hs-Sb-sbd+ transgene either with (+hs) or without (−hs) a double heat shock at 30 and 33 hr AP. Note short proximal segments decreasing in severity from femur to basitarsis in the legs from heat-shocked pupae. First legs show the most extreme effect.
