Figure 6.

Larval and prepupal leg discs stained with stubblin antistem antiserum. (A and E) Wild-type leg discs dissected at 3.5 hr AP show staining at the apical surface. (E) A confocal image; (A–D and F) conventional epifluorescence. (B) Negative control (no primary antibody). (D) Sb^63b/Sb^63b leg discs dissected at 3.5 hr AP show a similar pattern but more intense staining, consistent with the overexpression of the Sb^63b transcript. Sb^63b/Sb^63b discs have not elongated as far as their wild-type counterparts. (C and F) Larval discs dissected prior to normal endogenous Sb-sbd expression from animals carrying two copies of the wild-type Sb-sbd transgene: (C) larvae with no heat shock and (F) larvae heat shocked for 60 min at 37°. Heat-shocked larvae show precocious apical expression, visible as concentric rings in the uneverted disc. Bars, 50 μm.