Abstract
The solution conformations of the dinucleotide d(TT) and the modified duplex d(CGCGAATTCGCG)2 with N3'--> P5' phosphoramidate internucleoside linkages have been studied using circular dichroism (CD) and NMR spectroscopy. The CD spectra indicate that the duplex conformation is similar to that of isosequential phosphodiester RNA, a A-type helix, and is different from that of DNA, a B-type helix, NMR studies of model dimers d(TpT) and N3'--> P5' phosphoramidate d(TnpT) show that the sugar ring conformation changes from predominantly C2'-endo to C3'-endo when the 3'-phosphoester is replaced by a phosphoramidate group. Two-dimensional NMR (NOESY, DQF-COSY and TOCSY spectra) studies of the duplex provide additional details about the A-type duplex conformation of the oligonucleotide phosphoramidate and confirm that all furanose rings of 3'-aminonucleotides adopt predominantly N-type sugar puckering.
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- Bayever E., Iversen P. L., Bishop M. R., Sharp J. G., Tewary H. K., Arneson M. A., Pirruccello S. J., Ruddon R. W., Kessinger A., Zon G. Systemic administration of a phosphorothioate oligonucleotide with a sequence complementary to p53 for acute myelogenous leukemia and myelodysplastic syndrome: initial results of a phase I trial. Antisense Res Dev. 1993 Winter;3(4):383–390. doi: 10.1089/ard.1993.3.383. [DOI] [PubMed] [Google Scholar]
- Chou S. H., Flynn P., Reid B. Solid-phase synthesis and high-resolution NMR studies of two synthetic double-helical RNA dodecamers: r(CGCGAAUUCGCG) and r(CGCGUAUACGCG). Biochemistry. 1989 Mar 21;28(6):2422–2435. doi: 10.1021/bi00432a013. [DOI] [PubMed] [Google Scholar]
- Dickerson R. E., Drew H. R., Conner B. N., Wing R. M., Fratini A. V., Kopka M. L. The anatomy of A-, B-, and Z-DNA. Science. 1982 Apr 30;216(4545):475–485. doi: 10.1126/science.7071593. [DOI] [PubMed] [Google Scholar]
- Kibler-Herzog L., Zon G., Uznanski B., Whittier G., Wilson W. D. Duplex stabilities of phosphorothioate, methylphosphonate, and RNA analogs of two DNA 14-mers. Nucleic Acids Res. 1991 Jun 11;19(11):2979–2986. doi: 10.1093/nar/19.11.2979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lane A. N., Jenkins T. C., Brown T., Neidle S. Interaction of berenil with the EcoRI dodecamer d(CGCGAATTCGCG)2 in solution studied by NMR. Biochemistry. 1991 Feb 5;30(5):1372–1385. doi: 10.1021/bi00219a030. [DOI] [PubMed] [Google Scholar]
- Li Y., Zon G., Wilson W. D. Thermodynamics of DNA duplexes with adjacent G.A mismatches. Biochemistry. 1991 Jul 30;30(30):7566–7572. doi: 10.1021/bi00244a028. [DOI] [PubMed] [Google Scholar]
- Radhakrishnan I., Patel D. J. DNA triplexes: solution structures, hydration sites, energetics, interactions, and function. Biochemistry. 1994 Sep 27;33(38):11405–11416. doi: 10.1021/bi00204a001. [DOI] [PubMed] [Google Scholar]
- Riazance J. H., Baase W. A., Johnson W. C., Jr, Hall K., Cruz P., Tinoco I., Jr Evidence for Z-form RNA by vacuum UV circular dichroism. Nucleic Acids Res. 1985 Jul 11;13(13):4983–4989. doi: 10.1093/nar/13.13.4983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rinkel L. J., Altona C. Conformational analysis of the deoxyribofuranose ring in DNA by means of sums of proton-proton coupling constants: a graphical method. J Biomol Struct Dyn. 1987 Feb;4(4):621–649. doi: 10.1080/07391102.1987.10507665. [DOI] [PubMed] [Google Scholar]
- Tran-Dinh S., Neumann J. M., Taboury J., Huynh-Dinh T., Renous S., Genissel B., Igolen J. DNA fragment conformations. 1H-NMR comparative studies of helix-coil transition and conformation of d(C-A-C-G-T-G) and d(G-T-G-C-A-C). Influence of helix formation on proton chemical shifts. Eur J Biochem. 1983 Jul 1;133(3):579–589. doi: 10.1111/j.1432-1033.1983.tb07502.x. [DOI] [PubMed] [Google Scholar]
- Wilson W. D., Mizan S., Tanious F. A., Yao S., Zon G. The interaction of intercalators and groove-binding agents with DNA triple-helical structures: the influence of ligand structure, DNA backbone modifications and sequence. J Mol Recognit. 1994 Jun;7(2):89–98. doi: 10.1002/jmr.300070206. [DOI] [PubMed] [Google Scholar]
- Zuo E. T., Tanious F. A., Wilson W. D., Zon G., Tan G. S., Wartell R. M. Effect of base-pair sequence on the conformations and thermally induced transitions in oligodeoxyribonucleotides containing only AT base pairs. Biochemistry. 1990 May 8;29(18):4446–4456. doi: 10.1021/bi00470a027. [DOI] [PubMed] [Google Scholar]