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. 2006 Apr;172(4):2169–2184. doi: 10.1534/genetics.105.052738

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Copyright © 2006 by the Genetics Society of America

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Figure 6. — The C-terminal truncation mutant rgr1-Δ2 strongly enhances the basal transcription of both hsp82-ΔHSE1 and wild-type HSP genes, while drastically reducing their induced transcription. (A) hsp82-ΔHSE1/lacZ (β-gal) expression levels in RGR1⁺ (HS1004) and rgr1-Δ2 (SBK501) strains under noninducing and heat-shock-inducing conditions were determined as in Figure 3. Shown are means ±SEM (n = 4). (B) Dynamic range of expression of the strains analyzed in A. Dashed line indicates a level of 1.0 (no induction). (C and D). Northern analyses of RGR1⁺ (DY150) and rgr1-Δ2 (DY2694) strains maintained at 30° (C) or subjected to a 15-min 39° heat shock (D) using HSP-specific probes as in Figure 5. Illustrated are means ±SD (n = 2 or 3). (E) Dynamic range of heat-shock gene expression of RGR1⁺ and rgr1-Δ2 strains (data from C and D).

Figure 6. — The C-terminal truncation mutant rgr1-Δ2 strongly enhances the basal transcription of both hsp82-ΔHSE1 and wild-type HSP genes, while drastically reducing their induced transcription. (A) hsp82-ΔHSE1/lacZ (β-gal) expression levels in RGR1⁺ (HS1004) and rgr1-Δ2 (SBK501) strains under noninducing and heat-shock-inducing conditions were determined as in Figure 3. Shown are means ±SEM (n = 4). (B) Dynamic range of expression of the strains analyzed in A. Dashed line indicates a level of 1.0 (no induction). (C and D). Northern analyses of RGR1⁺ (DY150) and rgr1-Δ2 (DY2694) strains maintained at 30° (C) or subjected to a 15-min 39° heat shock (D) using HSP-specific probes as in Figure 5. Illustrated are means ±SD (n = 2 or 3). (E) Dynamic range of heat-shock gene expression of RGR1⁺ and rgr1-Δ2 strains (data from C and D).