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. 1996 May;88(1):20–27. doi: 10.1046/j.1365-2567.1996.d01-636.x

Ability of spleen, peritoneal cavity, and lymph node B cells to reconstitute serum immunoglobulin in SCID mice.

J Riggs 1, R Stowers 1
PMCID: PMC1456461  PMID: 8707345

Abstract

The impact of intrinsic B lymphocyte heterogeneity and of microenvironmental influences on serum immunoglobulin production by B cells was examined by intravenous (i.v.) and intraperitoneal (i.p.) transfer of BALB/c and BALB.xid (X-chromosome-linked immunedefective; XID) lymph node (LN), splenic (SP) and peritoneal cavity (PerC) cells into severe-combined immune-defective (SCID) mice. The results indicate that each B-cell source restores all immunoglobulin classes within 5 weeks of transfer, the rates for each isotype, however, differ between the B-cell sources. Serum IgM levels were restored most rapidly by PerC cell transfer, followed by SP and LN cell transfer. In addition, normal immunoglobulin levels were reached in the absence of complete lymphoid reconstitution. Serum immunoglobulin phenotypes characteristic of the donor strain, e.g. reduced IgM and IgG3 production by XID B cells, were maintained after transfer into the SCID recipient. Microenvironmental influences were indicated by reduced immunoglobulin production after i.p. transfer and after i.v. transfer into irradiated SCID recipients. The data show that both B-cell type and microenvironment play significant roles in generating the heterogeneous pool of B cells required for humoral immunity.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bosma G. C., Custer R. P., Bosma M. J. A severe combined immunodeficiency mutation in the mouse. Nature. 1983 Feb 10;301(5900):527–530. doi: 10.1038/301527a0. [DOI] [PubMed] [Google Scholar]
  2. Carroll A. M., Hardy R. R., Bosma M. J. Occurrence of mature B (IgM+, B220+) and T (CD3+) lymphocytes in scid mice. J Immunol. 1989 Aug 15;143(4):1087–1093. [PubMed] [Google Scholar]
  3. Chesebro B., Metzger H. Affinity labeling of a phosphorylcholine binding mouse myeloma protein. Biochemistry. 1972 Feb 29;11(5):766–771. doi: 10.1021/bi00755a014. [DOI] [PubMed] [Google Scholar]
  4. Dorshkind K., Pollack S. B., Bosma M. J., Phillips R. A. Natural killer (NK) cells are present in mice with severe combined immunodeficiency (scid). J Immunol. 1985 Jun;134(6):3798–3801. [PubMed] [Google Scholar]
  5. Freitas A. A., Rocha B., Coutinho A. A. Two major classes of mitogen-reactive B lymphocytes defined by life span. J Immunol. 1986 Jan;136(2):466–469. [PubMed] [Google Scholar]
  6. Fulop G. M., Phillips R. A. The scid mutation in mice causes a general defect in DNA repair. Nature. 1990 Oct 4;347(6292):479–482. doi: 10.1038/347479a0. [DOI] [PubMed] [Google Scholar]
  7. Förster I., Rajewsky K. Expansion and functional activity of Ly-1+ B cells upon transfer of peritoneal cells into allotype-congenic, newborn mice. Eur J Immunol. 1987 Apr;17(4):521–528. doi: 10.1002/eji.1830170414. [DOI] [PubMed] [Google Scholar]
  8. Hardy R. R., Hayakawa K., Haaijman J., Herzenberg L. A. B-cell subpopulations identified by two-colour fluorescence analysis. Nature. 1982 Jun 17;297(5867):589–591. doi: 10.1038/297589a0. [DOI] [PubMed] [Google Scholar]
  9. Hardy R. R., Hayakawa K., Parks D. R., Herzenberg L. A., Herzenberg L. A. Murine B cell differentiation lineages. J Exp Med. 1984 Apr 1;159(4):1169–1188. doi: 10.1084/jem.159.4.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hayakawa K., Hardy R. R., Parks D. R., Herzenberg L. A. The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice. J Exp Med. 1983 Jan 1;157(1):202–218. doi: 10.1084/jem.157.1.202. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Herzenberg L. A., Stall A. M., Lalor P. A., Sidman C., Moore W. A., Parks D. R., Herzenberg L. A. The Ly-1 B cell lineage. Immunol Rev. 1986 Oct;93:81–102. doi: 10.1111/j.1600-065x.1986.tb01503.x. [DOI] [PubMed] [Google Scholar]
  12. Inman J. K. Thymus-independent antigens: the preparation of covalent, hapten-ficoll conjugates. J Immunol. 1975 Feb;114(2 Pt 1):704–709. [PubMed] [Google Scholar]
  13. Klinman N. R., Linton P. J. The clonotype repertoire of B cell subpopulations. Adv Immunol. 1988;42:1–93. doi: 10.1016/s0065-2776(08)60842-1. [DOI] [PubMed] [Google Scholar]
  14. Lalor P. A., Herzenberg L. A., Adams S., Stall A. M. Feedback regulation of murine Ly-1 B cell development. Eur J Immunol. 1989 Mar;19(3):507–513. doi: 10.1002/eji.1830190315. [DOI] [PubMed] [Google Scholar]
  15. Martino G., Anastasi J., Feng J., Mc Shan C., DeGroot L., Quintans J., Grimaldi L. M. The fate of human peripheral blood lymphocytes after transplantation into SCID mice. Eur J Immunol. 1993 May;23(5):1023–1028. doi: 10.1002/eji.1830230506. [DOI] [PubMed] [Google Scholar]
  16. McIntyre T. M., Holmes K. L., Steinberg A. D., Kastner D. L. CD5+ peritoneal B cells express high levels of membrane, but not secretory, C mu mRNA. J Immunol. 1991 May 15;146(10):3639–3645. [PubMed] [Google Scholar]
  17. Mond J. J., Scher I., Cossman J., Kessler S., Mongini P. K., Hansen C., Finkelman F. D., Paul W. E. Role of the thymus in directing the development of a subset of B lymphocytes. J Exp Med. 1982 Mar 1;155(3):924–936. doi: 10.1084/jem.155.3.924. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mosmann T. R., Cherwinski H., Bond M. W., Giedlin M. A., Coffman R. L. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol. 1986 Apr 1;136(7):2348–2357. [PubMed] [Google Scholar]
  19. Pereira P., Forni L., Larsson E. L., Cooper M., Heusser C., Coutinho A. Autonomous activation of B and T cells in antigen-free mice. Eur J Immunol. 1986 Jun;16(6):685–688. doi: 10.1002/eji.1830160616. [DOI] [PubMed] [Google Scholar]
  20. Perlmutter R. M., Nahm M., Stein K. E., Slack J., Zitron I., Paul W. E., Davie J. M. Immunoglobulin subclass-specific immunodeficiency in mice with an X-linked B-lymphocyte defect. J Exp Med. 1979 Apr 1;149(4):993–998. doi: 10.1084/jem.149.4.993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Reininger L., Ollier P., Poncet P., Kaushik A., Jaton J. C. Novel V genes encode virtually identical variable regions of six murine monoclonal anti-bromelain-treated red blood cell autoantibodies. J Immunol. 1987 Jan 1;138(1):316–323. [PubMed] [Google Scholar]
  22. Riggs J. E., Hobbs M. V., Mosier D. E. CD4+CD8- thymocytes from neonatal mice induce IgM production in SCID mice. J Immunol. 1992 Mar 1;148(5):1389–1395. [PubMed] [Google Scholar]
  23. Riggs J. E., Stowers R. S., Mosier D. E. The immunoglobulin allotype contributed by peritoneal cavity B cells dominates in SCID mice reconstituted with allotype-disparate mixtures of splenic and peritoneal cavity B cells. J Exp Med. 1990 Aug 1;172(2):475–485. doi: 10.1084/jem.172.2.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Snapper C. M., Yamaguchi H., Moorman M. A., Sneed R., Smoot D., Mond J. J. Natural killer cells induce activated murine B cells to secrete Ig. J Immunol. 1993 Nov 15;151(10):5251–5260. [PubMed] [Google Scholar]
  25. Thomas-Vaslin V., Coutinho A., Huetz F. Origin of CD5+ B cells and natural IgM-secreting cells: reconstitution potential of adult bone marrow, spleen and peritoneal cells. Eur J Immunol. 1992 May;22(5):1243–1251. doi: 10.1002/eji.1830220520. [DOI] [PubMed] [Google Scholar]
  26. Wortis H. H., Teutsch M., Higer M., Zheng J., Parker D. C. B-cell activation by crosslinking of surface IgM or ligation of CD40 involves alternative signal pathways and results in different B-cell phenotypes. Proc Natl Acad Sci U S A. 1995 Apr 11;92(8):3348–3352. doi: 10.1073/pnas.92.8.3348. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. von Boehmer H. The developmental biology of T lymphocytes. Annu Rev Immunol. 1988;6:309–326. doi: 10.1146/annurev.iy.06.040188.001521. [DOI] [PubMed] [Google Scholar]

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