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. 1996 May;88(1):98–103. doi: 10.1046/j.1365-2567.1996.d01-640.x

Expression of major histocompatibility complex class II antigens on normal porcine intestinal endothelium.

A D Wilson 1, K Haverson 1, K Southgate 1, P W Bland 1, C R Stokes 1, M Bailey 1
PMCID: PMC1456475  PMID: 8707357

Abstract

A novel monoclonal antibody (MIL 11) specific for an antigen expressed on porcine endothelial cells is described. The antigen recognized by MIL 11 is most strongly expressed in the intestine but is also expressed on the capillary endothelium of a wide range of tissues. Using two- and three-colour immunofluorescence microscopy we demonstrated the extensive coexpression of MIL 11 and major histocompatibility complex (MHC) class II antigens on normal porcine capillary endothelium in the intestine, trachea, thymus and small veins, while endothelium of large vessels and the heart were negative for MHC class II. In contrast to humans and rodents, available reagents do not detect MHC class II on the intestinal epithelium of pigs. However, porcine intestinal endothelium expressed both DR and DQ antigens. A population of strongly class II-positive cells was also detected immediately adjacent to the endothelium in the lamina propria. Three-colour immunofluorescence microscopy highlighted the close association between endothelium and intestinal CD4+ T cells. Lamina propria T cells were mainly MHC class II positive, whereas those in the epithelial compartment were MHC class II negative.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bailey M., Hall L., Bland P. W., Stokes C. R. Production of cytokines by lymphocytes from spleen, mesenteric lymph node and intestinal lamina propria of pigs. Immunology. 1994 Aug;82(4):577–583. [PMC free article] [PubMed] [Google Scholar]
  2. Binns R. M., Licence S. T., Wooding F. B., Duffus W. P. Active lymphocyte traffic induced in the periphery by cytokines and phytohemagglutinin: three different mechanisms? Eur J Immunol. 1992 Sep;22(9):2195–2203. doi: 10.1002/eji.1830220903. [DOI] [PubMed] [Google Scholar]
  3. Binns R. M., Pabst R., Licence S. T. Lymphocyte emigration from lymph nodes by blood in the pig and efferent lymph in the sheep. Immunology. 1985 Jan;54(1):105–111. [PMC free article] [PubMed] [Google Scholar]
  4. Damle N. K., Klussman K., Linsley P. S., Aruffo A. Differential costimulatory effects of adhesion molecules B7, ICAM-1, LFA-3, and VCAM-1 on resting and antigen-primed CD4+ T lymphocytes. J Immunol. 1992 Apr 1;148(7):1985–1992. [PubMed] [Google Scholar]
  5. Dustin M. L., Springer T. A. T-cell receptor cross-linking transiently stimulates adhesiveness through LFA-1. Nature. 1989 Oct 19;341(6243):619–624. doi: 10.1038/341619a0. [DOI] [PubMed] [Google Scholar]
  6. Koyama K., Fukunishi T., Barcos M., Tanigaki N., Pressman D. Human Ia-like antigens in non-lymphoid organs. Immunology. 1979 Oct;38(2):333–341. [PMC free article] [PubMed] [Google Scholar]
  7. Lasky L. A. Selectins: interpreters of cell-specific carbohydrate information during inflammation. Science. 1992 Nov 6;258(5084):964–969. doi: 10.1126/science.1439808. [DOI] [PubMed] [Google Scholar]
  8. Liu Y., Janeway C. A., Jr Cells that present both specific ligand and costimulatory activity are the most efficient inducers of clonal expansion of normal CD4 T cells. Proc Natl Acad Sci U S A. 1992 May 1;89(9):3845–3849. doi: 10.1073/pnas.89.9.3845. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lunney J. K. Characterization of swine leukocyte differentiation antigens. Immunol Today. 1993 Apr;14(4):147–148. doi: 10.1016/0167-5699(93)90227-C. [DOI] [PubMed] [Google Scholar]
  10. Lynch S. E., Colvin R. B., Antoniades H. N. Growth factors in wound healing. Single and synergistic effects on partial thickness porcine skin wounds. J Clin Invest. 1989 Aug;84(2):640–646. doi: 10.1172/JCI114210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McCarron R. M., Wang L., Cowan E. P., Spatz M. Class II MHC antigen expression by cultured human cerebral vascular endothelial cells. Brain Res. 1991 Dec 6;566(1-2):325–328. doi: 10.1016/0006-8993(91)91718-g. [DOI] [PubMed] [Google Scholar]
  12. Morrison L. A., Lukacher A. E., Braciale V. L., Fan D. P., Braciale T. J. Differences in antigen presentation to MHC class I-and class II-restricted influenza virus-specific cytolytic T lymphocyte clones. J Exp Med. 1986 Apr 1;163(4):903–921. doi: 10.1084/jem.163.4.903. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Mueller D. L., Jenkins M. K., Schwartz R. H. Clonal expansion versus functional clonal inactivation: a costimulatory signalling pathway determines the outcome of T cell antigen receptor occupancy. Annu Rev Immunol. 1989;7:445–480. doi: 10.1146/annurev.iy.07.040189.002305. [DOI] [PubMed] [Google Scholar]
  14. Picker L. J., Butcher E. C. Physiological and molecular mechanisms of lymphocyte homing. Annu Rev Immunol. 1992;10:561–591. doi: 10.1146/annurev.iy.10.040192.003021. [DOI] [PubMed] [Google Scholar]
  15. Pober J. S., Collins T., Gimbrone M. A., Jr, Cotran R. S., Gitlin J. D., Fiers W., Clayberger C., Krensky A. M., Burakoff S. J., Reiss C. S. Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon. Nature. 1983 Oct 20;305(5936):726–729. doi: 10.1038/305726a0. [DOI] [PubMed] [Google Scholar]
  16. Rosengard B. R., Kortz E. O., Ojikutu C. A., Guzzetta P. C., Sundt T. M., 3rd, Smith C. V., Nakajima K., Boorstein S. M., Hill G. S., Sachs D. H. The failure of skin grafting to break tolerance to class I-disparate renal allografts in miniature swine despite inducing marked antidonor cellular immunity. Transplantation. 1991 Dec;52(6):1044–1052. doi: 10.1097/00007890-199112000-00020. [DOI] [PubMed] [Google Scholar]
  17. Springer T. A. Adhesion receptors of the immune system. Nature. 1990 Aug 2;346(6283):425–434. doi: 10.1038/346425a0. [DOI] [PubMed] [Google Scholar]
  18. St Louis J. D., Lederer J. A., Lichtman A. H. Costimulator deficient antigen presentation by an endothelial cell line induces a nonproliferative T cell activation response without anergy. J Exp Med. 1993 Nov 1;178(5):1597–1605. doi: 10.1084/jem.178.5.1597. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stoolman L. M. Adhesion molecules controlling lymphocyte migration. Cell. 1989 Mar 24;56(6):907–910. doi: 10.1016/0092-8674(89)90620-x. [DOI] [PubMed] [Google Scholar]
  20. Wilson A. D., Bailey M., Williams N. A., Stokes C. R. The in vitro production of cytokines by mucosal lymphocytes immunized by oral administration of keyhole limpet hemocyanin using cholera toxin as an adjuvant. Eur J Immunol. 1991 Oct;21(10):2333–2339. doi: 10.1002/eji.1830211007. [DOI] [PubMed] [Google Scholar]
  21. de Waal R. M., Bogman M. J., Maass C. N., Cornelissen L. M., Tax W. J., Koene R. A. Variable expression of Ia antigens on the vascular endothelium of mouse skin allografts. Nature. 1983 Jun 2;303(5916):426–429. doi: 10.1038/303426a0. [DOI] [PubMed] [Google Scholar]

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