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. 1996 Oct;89(2):178–182. doi: 10.1046/j.1365-2567.1996.d01-738.x

Salivary IgG subclasses in individuals with and without homozygous IGHG gene deletions.

P E Engström 1, G Norhagen 1, L Osipova 1, A Helal 1, V Wiebe 1, A Brusco 1, A O Carbonara 1, G Lefranc 1, M P Lefranc 1
PMCID: PMC1456487  PMID: 8943711

Abstract

In this study, the levels of salivary IgG1, IgG2, IgG3 and IgG4 from individuals with and without homozygous immunoglobulin heavy chain constant gene deletions were quantified by enzyme-linked immunosorbent assay (ELISA). To analyse the restriction of salivary IgG subclasses, we used unstimulated whole saliva and sera collected at the same time from individuals with homozygous gene deletions, two with G1 deletion, one with G4 deletion, six with both G2 and G4 deletions and from eight individuals without IGHG gene deletions and expressing all four IgG subclasses. The median values of salivary IgG from individuals with homozygous G1, or G4, or both G2 and G4 deletions, and from individuals expressing all four subclasses were 24.2 mg/l and 23.4 mg/l, respectively. The median values of serum IgG were 13.7 g/l and 15.9 g/l, respectively. Our results show that the salivary and serum IgG levels were both within the normal range in individuals with homozygous gene deletions of either G1, or G4, or both G2 and G4.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aalberse R. C., van der Gaag R., van Leeuwen J. Serologic aspects of IgG4 antibodies. I. Prolonged immunization results in an IgG4-restricted response. J Immunol. 1983 Feb;130(2):722–726. [PubMed] [Google Scholar]
  2. Bottaro A., De Marchi M., De Lange G., Boccazzi C., Caldesi F., Gallina R., Carbonara A. O. New types of multiple and single gene deletions in the human IgCH locus. Immunogenetics. 1989;29(1):44–48. doi: 10.1007/BF02341612. [DOI] [PubMed] [Google Scholar]
  3. Engström P. E., Norhagen G., Bottaro A., Carbonara A. O., Lefranc G., Steinitz M., Söder P. O., Smith C. I., Hammarström L. Subclass distribution of antigen-specific IgA antibodies in normal donors and individuals with homozygous C alpha 1 or C alpha 2 gene deletions. J Immunol. 1990 Jul 1;145(1):109–116. [PubMed] [Google Scholar]
  4. Flanagan J. G., Rabbitts T. H. Arrangement of human immunoglobulin heavy chain constant region genes implies evolutionary duplication of a segment containing gamma, epsilon and alpha genes. Nature. 1982 Dec 23;300(5894):709–713. doi: 10.1038/300709a0. [DOI] [PubMed] [Google Scholar]
  5. French M. A., Harrison G. Serum IgG subclass concentrations in healthy adults: a study using monoclonal antisera. Clin Exp Immunol. 1984 May;56(2):473–475. [PMC free article] [PubMed] [Google Scholar]
  6. Kim K., Keller M. A., Heiner D. C. Immunoglobulin G subclasses in human colostrum, milk and saliva. Acta Paediatr. 1992 Feb;81(2):113–118. doi: 10.1111/j.1651-2227.1992.tb12185.x. [DOI] [PubMed] [Google Scholar]
  7. Lefranc G., Chaabani H., Van Loghem E., Lefranc M. P., De Lange G., Helal A. N. Simultaneous absence of the human IgG1, IgG2, IgG4 and IgA1 subclasses: immunological and immunogenetical considerations. Eur J Immunol. 1983 Mar;13(3):240–244. doi: 10.1002/eji.1830130312. [DOI] [PubMed] [Google Scholar]
  8. Lefranc M. P., Lefranc G., Rabbitts T. H. Inherited deletion of immunoglobulin heavy chain constant region genes in normal human individuals. Nature. 1982 Dec 23;300(5894):760–762. doi: 10.1038/300760a0. [DOI] [PubMed] [Google Scholar]
  9. Lefranc M. P., Lefranc G., de Lange G., Out T. A., van den Broek P. J., van Nieuwkoop J., Radl J., Helal A. N., Chaabani H., van Loghem E. Instability of the human immunoglobulin heavy chain constant region locus indicated by different inherited chromosomal deletions. Mol Biol Med. 1983 Sep;1(2):207–217. [PubMed] [Google Scholar]
  10. Migone N., Oliviero S., de Lange G., Delacroix D. L., Boschis D., Altruda F., Silengo L., DeMarchi M., Carbonara A. O. Multiple gene deletions within the human immunoglobulin heavy-chain cluster. Proc Natl Acad Sci U S A. 1984 Sep;81(18):5811–5815. doi: 10.1073/pnas.81.18.5811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Milstein C. P., Deverson E. V., Rabbitts T. H. The sequence of the human immunoglobulin mu-delta intron reveals possible vestigial switch segments. Nucleic Acids Res. 1984 Aug 24;12(16):6523–6535. doi: 10.1093/nar/12.16.6523. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Norhagen G., Engström P. E., Hammarström L., Söder P. O., Smith C. I. Immunoglobulin levels in saliva in individuals with selective IgA deficiency: compensatory IgM secretion and its correlation with HLA and susceptibility to infections. J Clin Immunol. 1989 Jul;9(4):279–286. doi: 10.1007/BF00918659. [DOI] [PubMed] [Google Scholar]
  13. Persson M. A., Hammarström L., Smith C. I. Enzyme-linked immunosorbent assay for subclass distribution of human IgG and IgA antigen-specific antibodies. J Immunol Methods. 1985 Apr 8;78(1):109–121. doi: 10.1016/0022-1759(85)90334-5. [DOI] [PubMed] [Google Scholar]
  14. Plebani A., Ugazio A. G., Meini A., Ruggeri L., Negrini A., Albertini A., Leibovitz M., Duse M., Bottaro A., Brusco R. Extensive deletion of immunoglobulin heavy chain constant region genes in the absence of recurrent infections: when is IgG subclass deficiency clinically relevant? Clin Immunol Immunopathol. 1993 Jul;68(1):46–50. doi: 10.1006/clin.1993.1093. [DOI] [PubMed] [Google Scholar]
  15. Reunala T., Brummer-Korvenkontio H., Palosuo K., Miyanij M., Ruiz-Maldonado R., Löve A., François G., Palosuo T. Frequent occurrence of IgE and IgG4 antibodies against saliva of Aedes communis and Aedes aegypti mosquitoes in children. Int Arch Allergy Immunol. 1994 Aug;104(4):366–371. doi: 10.1159/000236693. [DOI] [PubMed] [Google Scholar]
  16. Urbanek R., Kemeny D. M., Richards D. Sub-class of IgG anti-bee venom antibody produced during bee venom immunotherapy and its relationship to long-term protection from bee stings and following termination of venom immunotherapy. Clin Allergy. 1986 Jul;16(4):317–322. doi: 10.1111/j.1365-2222.1986.tb01963.x. [DOI] [PubMed] [Google Scholar]
  17. Wiebe V., Helal A., Lefranc M. P., Lefranc G. Molecular analysis of the T17 immunoglobulin CH multigene deletion (del A1-GP-G2-G4-E). Hum Genet. 1994 May;93(5):520–528. doi: 10.1007/BF00202816. [DOI] [PubMed] [Google Scholar]

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