Skip to main content
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1996 Feb 1;24(3):534–535. doi: 10.1093/nar/24.3.534

An efficient method for stable transfection of bloodstream-form Trypanosoma brucei.

F Li 1, K M Gottesdiener 1
PMCID: PMC145656  PMID: 8602369

Full Text

The Full Text of this article is available as a PDF (33.2 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Carruthers V. B., van der Ploeg L. H., Cross G. A. DNA-mediated transformation of bloodstream-form Trypanosoma brucei. Nucleic Acids Res. 1993 May 25;21(10):2537–2538. doi: 10.1093/nar/21.10.2537. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chung H. M., Lee M. G., Dietrich P., Huang J., Van der Ploeg L. H. Disruption of largest subunit RNA polymerase II genes in Trypanosoma brucei. Mol Cell Biol. 1993 Jun;13(6):3734–3743. doi: 10.1128/mcb.13.6.3734. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Drocourt D., Calmels T., Reynes J. P., Baron M., Tiraby G. Cassettes of the Streptoalloteichus hindustanus ble gene for transformation of lower and higher eukaryotes to phleomycin resistance. Nucleic Acids Res. 1990 Jul 11;18(13):4009–4009. doi: 10.1093/nar/18.13.4009. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hesse F., Selzer P. M., Mühlstädt K., Duszenko M. A novel cultivation technique for long-term maintenance of bloodstream form trypanosomes in vitro. Mol Biochem Parasitol. 1995 Mar;70(1-2):157–166. doi: 10.1016/0166-6851(95)00027-x. [DOI] [PubMed] [Google Scholar]
  5. Hirumi H., Hirumi K. Continuous cultivation of Trypanosoma brucei blood stream forms in a medium containing a low concentration of serum protein without feeder cell layers. J Parasitol. 1989 Dec;75(6):985–989. [PubMed] [Google Scholar]
  6. Jefferies D., Tebabi P., Le Ray D., Pays E. The ble resistance gene as a new selectable marker for Trypanosoma brucei: fly transmission of stable procyclic transformants to produce antibiotic resistant bloodstream forms. Nucleic Acids Res. 1993 Jan 25;21(2):191–195. doi: 10.1093/nar/21.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lee M. G., Van der Ploeg L. H. Homologous recombination and stable transfection in the parasitic protozoan Trypanosoma brucei. Science. 1990 Dec 14;250(4987):1583–1587. doi: 10.1126/science.2177225. [DOI] [PubMed] [Google Scholar]
  8. Phillips M. A., Coffino P., Wang C. C. Cloning and sequencing of the ornithine decarboxylase gene from Trypanosoma brucei. Implications for enzyme turnover and selective difluoromethylornithine inhibition. J Biol Chem. 1987 Jun 25;262(18):8721–8727. [PubMed] [Google Scholar]
  9. ten Asbroek A. L., Ouellette M., Borst P. Targeted insertion of the neomycin phosphotransferase gene into the tubulin gene cluster of Trypanosoma brucei. Nature. 1990 Nov 8;348(6297):174–175. doi: 10.1038/348174a0. [DOI] [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES