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. 1996 Nov;89(3):316–323. doi: 10.1046/j.1365-2567.1996.d01-761.x

Molecular characterization of the V regions of four IgE antibodies specific for trichosanthin.

Y Wang 1, M Yeh 1
PMCID: PMC1456560  PMID: 8958042

Abstract

We report here the cDNA cloning and variable region nucleic acid sequences of the heavy and the light chains of four monoclonal IgE and one monoclonal IgG1 antibodies against trichosanthin (TCS), all of which recognized a similar or related antigenic determinant. Sequence analysis revealed that the VH regions of the four IgE antibodies use different V gene segments from the 7183, 36-60 and J558 families. The DH genes varied extensively in nucleotide sequence and length, with D segments DSP2, DFL16 and DQ52 being used with equal frequency. All JH gene segments except JH1 were present in the epsilon transcripts. In contrast, there appeared to be restricted L-chain utilization. All four L-chain V genes cloned belonged to the V kappa 21 family and a bias in the use of J kappa 1 gene was also observed. The VL sequences of the anti-TCS monoclonal antibodies showed much less diversity than the VH sequences. We propose that the L-chain rather than H-chain may play a more significant role in specific binding with allergenic determinant on TCS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alanen A., Weiss S. Sequence and linkage of the V kappa 21A and G germ-line gene segments in the mouse. Eur J Immunol. 1989 Oct;19(10):1961–1963. doi: 10.1002/eji.1830191031. [DOI] [PubMed] [Google Scholar]
  2. Buenafe A. C., Makowski F. F., Rittenberg M. B. Molecular analysis and fine specificity of antibodies against an organophosphorus hapten. J Immunol. 1989 Jul 15;143(2):539–545. [PubMed] [Google Scholar]
  3. Chomczynski P., Sacchi N. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987 Apr;162(1):156–159. doi: 10.1006/abio.1987.9999. [DOI] [PubMed] [Google Scholar]
  4. Chukwuocha R. U., Hartman A. B., Feeney A. J. Sequences of four new members of the VH7183 gene family in BALB/c mice. Immunogenetics. 1994;40(1):76–78. doi: 10.1007/BF00163968. [DOI] [PubMed] [Google Scholar]
  5. Collins E. J., Robertus J. D., LoPresti M., Stone K. L., Williams K. R., Wu P., Hwang K., Piatak M. Primary amino acid sequence of alpha-trichosanthin and molecular models for abrin A-chain and alpha-trichosanthin. J Biol Chem. 1990 May 25;265(15):8665–8669. [PubMed] [Google Scholar]
  6. Gu H., Yeh M., Yao Z. Investigation of antigenic determinants on trichosanthin by antibody competitive binding assay. Shi Yan Sheng Wu Xue Bao. 1986 Mar;19(1):121–129. [PubMed] [Google Scholar]
  7. Gu H., Yeh M., Yao Z. Preparation, isolation and characterization of mouse IgE monoclonal antibodies against trichosanthin protein. Shi Yan Sheng Wu Xue Bao. 1986 Mar;19(1):109–118. [PubMed] [Google Scholar]
  8. Ji Y. Y., Jiang Z. Q., Yeh M. [The production of monoclonal antiidiotypic antibodies against trichosanthin-specific IgE by rat-mouse hybridomas]. Shi Yan Sheng Wu Xue Bao. 1986 Mar;19(1):91–98. [PubMed] [Google Scholar]
  9. Kurosawa Y., Tonegawa S. Organization, structure, and assembly of immunoglobulin heavy chain diversity DNA segments. J Exp Med. 1982 Jan 1;155(1):201–218. doi: 10.1084/jem.155.1.201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lötscher M., Blaser K., Heusser C. H. Specific V gene usage in anti-phosphorylcholine IgE antibodies. Mol Immunol. 1993 Dec;30(17):1573–1582. doi: 10.1016/0161-5890(93)90448-k. [DOI] [PubMed] [Google Scholar]
  11. Lötscher M., Heusser C. H., Amstutz H., Blaser K. Fine specificity and VJ usage of light chains in antibodies to the phosphorylcholine hapten. Eur J Immunol. 1993 Jan;23(1):124–130. doi: 10.1002/eji.1830230120. [DOI] [PubMed] [Google Scholar]
  12. Max E. E., Maizel J. V., Jr, Leder P. The nucleotide sequence of a 5.5-kilobase DNA segment containing the mouse kappa immunoglobulin J and C region genes. J Biol Chem. 1981 May 25;256(10):5116–5120. [PubMed] [Google Scholar]
  13. McGrath M. S., Hwang K. M., Caldwell S. E., Gaston I., Luk K. C., Wu P., Ng V. L., Crowe S., Daniels J., Marsh J. GLQ223: an inhibitor of human immunodeficiency virus replication in acutely and chronically infected cells of lymphocyte and mononuclear phagocyte lineage. Proc Natl Acad Sci U S A. 1989 Apr;86(8):2844–2848. doi: 10.1073/pnas.86.8.2844. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Orlandi R., Güssow D. H., Jones P. T., Winter G. Cloning immunoglobulin variable domains for expression by the polymerase chain reaction. Proc Natl Acad Sci U S A. 1989 May;86(10):3833–3837. doi: 10.1073/pnas.86.10.3833. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sablitzky F., Wildner G., Rajewsky K. Somatic mutation and clonal expansion of B cells in an antigen-driven immune response. EMBO J. 1985 Feb;4(2):345–350. doi: 10.1002/j.1460-2075.1985.tb03635.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sakano H., Maki R., Kurosawa Y., Roeder W., Tonegawa S. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes. Nature. 1980 Aug 14;286(5774):676–683. doi: 10.1038/286676a0. [DOI] [PubMed] [Google Scholar]
  17. Weigert M., Gatmaitan L., Loh E., Schilling J., Hood L. Rearrangement of genetic information may produce immunoglobulin diversity. Nature. 1978 Dec 21;276(5690):785–790. doi: 10.1038/276785a0. [DOI] [PubMed] [Google Scholar]
  18. Winter E., Radbruch A., Krawinkel U. Members of novel VH gene families are found in VDJ regions of polyclonally activated B-lymphocytes. EMBO J. 1985 Nov;4(11):2861–2867. doi: 10.1002/j.1460-2075.1985.tb04015.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yeh M., Jiang Z. Q., Gu H., Ji Y. Y. [In vitro induction of secondary anti-trichosanthin antibody responses by antiidiotype antibodies]. Shi Yan Sheng Wu Xue Bao. 1987 Sep;20(3):293–302. [PubMed] [Google Scholar]
  20. Zhang J. S., Liu W. Y. The mechanism of action of trichosanthin on eukaryotic ribosomes--RNA N-glycosidase activity of the cytotoxin. Nucleic Acids Res. 1992 Mar 25;20(6):1271–1275. doi: 10.1093/nar/20.6.1271. [DOI] [PMC free article] [PubMed] [Google Scholar]

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