Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1997 Apr;90(4):586–591. doi: 10.1046/j.1365-2567.1997.00202.x

Transforming growth factor-beta induced by live or ultraviolet-inactivated equid herpes virus type-1 mediates immunosuppression in the horse.

S Charan 1, K Palmer 1, P Chester 1, A R Mire-Sluis 1, A Meager 1, N Edington 1
PMCID: PMC1456683  PMID: 9176113

Abstract

Up to 21 days after exposure to live or ultraviolet-inactivated equid herpesvirus type-1 (EHV-1) autologous serum from ponies caused an immunosuppressive effect if incorporated into T-cell proliferation assays to EHV-1. The suppressive factor in the sera of ponies also inhibited T-cell response to phytohaemagglutinin. Increased levels of circulating activated transforming growth factor-beta 1 (TGF-beta 1) were detected, and the suppressive activity of the serum could be reversed by antibody to TGF-beta 1. In a challenge experiment the ponies which exhibited circulating TGF-beta 1 activity succumbed to infection while the ones with similar magnitudes of T-cell responses, but no TGF-beta 1 activity, were protected. A definition of this immunosuppressive mechanism and its mode of induction must be central to the design of vaccines and to an understanding of the pathogenesis of EHV-1.

Full text

PDF
586

Images in this article

589Figure 5
on p.589

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allen G. P., Bryans J. T. Molecular epizootiology, pathogenesis, and prophylaxis of equine herpesvirus-1 infections. Prog Vet Microbiol Immunol. 1986;2:78–144. [PubMed] [Google Scholar]
  2. Allison A. C. Interactions of T-and B-lymphocytes and macrophages in recovery from virus infections. Proc R Soc Med. 1973 Dec;66(12):1151–1154. doi: 10.1177/003591577306601201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bauer G., Götschl M., Höfler P. Tumor-promoting activity of Epstein-Barr-virus-inducing factor transforming growth factor type beta (EIF/TGF-beta) is due to the induction of irreversible transformation. Int J Cancer. 1991 Apr 1;47(6):881–888. doi: 10.1002/ijc.2910470616. [DOI] [PubMed] [Google Scholar]
  4. Bridges C. G., Edington N. Innate immunity during Equid herpesvirus 1 (EHV-1) infection. Clin Exp Immunol. 1986 Jul;65(1):172–181. [PMC free article] [PubMed] [Google Scholar]
  5. Burrows R., Goodridge D., Denyer M. S. Trials of an inactivated equid herpesvirus 1 vaccine: challenge with a subtype 1 virus. Vet Rec. 1984 Apr 14;114(15):369–374. doi: 10.1136/vr.114.15.369. [DOI] [PubMed] [Google Scholar]
  6. Cook R. F., O'Neill T., Strachan E., Sundquist B., Mumford J. A. Protection against lethal equine herpes virus type 1 (subtype 1) infection in hamsters by immune stimulating complexes (ISCOMs) containing the major viral glycoproteins. Vaccine. 1990 Oct;8(5):491–496. doi: 10.1016/0264-410x(90)90252-h. [DOI] [PubMed] [Google Scholar]
  7. Davignon J. L., Castanié P., Yorke J. A., Gautier N., Clément D., Davrinche C. Anti-human cytomegalovirus activity of cytokines produced by CD4+ T-cell clones specifically activated by IE1 peptides in vitro. J Virol. 1996 Apr;70(4):2162–2169. doi: 10.1128/jvi.70.4.2162-2169.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dutta S. K., Myrup A., Bumgardner M. K. Lymphocyte responses to virus and mitogen in ponies during experimental infection with equine herpesvirus 1. Am J Vet Res. 1980 Dec;41(12):2066–2068. [PubMed] [Google Scholar]
  9. Edington N., Bridges C. G., Griffiths L. Equine interferons following exposure to equid herpesvirus-1 or -4. J Interferon Res. 1989 Aug;9(4):389–392. doi: 10.1089/jir.1989.9.389. [DOI] [PubMed] [Google Scholar]
  10. Flanders K. C., Roberts A. B., Ling N., Fleurdelys B. E., Sporn M. B. Antibodies to peptide determinants in transforming growth factor beta and their applications. Biochemistry. 1988 Jan 26;27(2):739–746. doi: 10.1021/bi00402a037. [DOI] [PubMed] [Google Scholar]
  11. Fontana A., Frei K., Bodmer S., Hofer E., Schreier M. H., Palladino M. A., Jr, Zinkernagel R. M. Transforming growth factor-beta inhibits the generation of cytotoxic T cells in virus-infected mice. J Immunol. 1989 Nov 15;143(10):3230–3234. [PubMed] [Google Scholar]
  12. Grotendorst G. R., Smale G., Pencev D. Production of transforming growth factor beta by human peripheral blood monocytes and neutrophils. J Cell Physiol. 1989 Aug;140(2):396–402. doi: 10.1002/jcp.1041400226. [DOI] [PubMed] [Google Scholar]
  13. Guo P. X., Goebel S., Davis S., Perkus M. E., Languet B., Desmettre P., Allen G., Paoletti E. Expression in recombinant vaccinia virus of the equine herpesvirus 1 gene encoding glycoprotein gp13 and protection of immunized animals. J Virol. 1989 Oct;63(10):4189–4198. doi: 10.1128/jvi.63.10.4189-4198.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Heise M. T., Virgin H. W., 4th The T-cell-independent role of gamma interferon and tumor necrosis factor alpha in macrophage activation during murine cytomegalovirus and herpes simplex virus infections. J Virol. 1995 Feb;69(2):904–909. doi: 10.1128/jvi.69.2.904-909.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kuruvilla A. P., Shah R., Hochwald G. M., Liggitt H. D., Palladino M. A., Thorbecke G. J. Protective effect of transforming growth factor beta 1 on experimental autoimmune diseases in mice. Proc Natl Acad Sci U S A. 1991 Apr 1;88(7):2918–2921. doi: 10.1073/pnas.88.7.2918. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lowrance J. H., O'Sullivan F. X., Caver T. E., Waegell W., Gresham H. D. Spontaneous elaboration of transforming growth factor beta suppresses host defense against bacterial infection in autoimmune MRL/lpr mice. J Exp Med. 1994 Nov 1;180(5):1693–1703. doi: 10.1084/jem.180.5.1693. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Meager A. Assays for transforming growth factor beta. J Immunol Methods. 1991 Jul 26;141(1):1–14. doi: 10.1016/0022-1759(91)90204-s. [DOI] [PubMed] [Google Scholar]
  18. Michelson S., Alcami J., Kim S. J., Danielpour D., Bachelerie F., Picard L., Bessia C., Paya C., Virelizier J. L. Human cytomegalovirus infection induces transcription and secretion of transforming growth factor beta 1. J Virol. 1994 Sep;68(9):5730–5737. doi: 10.1128/jvi.68.9.5730-5737.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Mumford J. A., Bates J. Trials of an inactivated equid herpesvirus 1 vaccine: challenge with a subtype 2 virus. Vet Rec. 1984 Apr 14;114(15):375–381. doi: 10.1136/vr.114.15.375. [DOI] [PubMed] [Google Scholar]
  20. Mumford J. A., Rossdale P. D., Jessett D. M., Gann S. J., Ousey J., Cook R. F. Serological and virological investigations of an equid herpesvirus 1 (EHV-1) abortion storm on a stud farm in 1985. J Reprod Fertil Suppl. 1987;35:509–518. [PubMed] [Google Scholar]
  21. O'Hagan D. T., Jeffery H., Roberts M. J., McGee J. P., Davis S. S. Controlled release microparticles for vaccine development. Vaccine. 1991 Oct;9(10):768–771. doi: 10.1016/0264-410x(91)90295-h. [DOI] [PubMed] [Google Scholar]
  22. Patel J. R., Edington N., Mumford J. A. Variation in cellular tropism between isolates of equine herpesvirus-1 in foals. Arch Virol. 1982;74(1):41–51. doi: 10.1007/BF01320781. [DOI] [PubMed] [Google Scholar]
  23. Rinaldo C. R., Jr, Torpey D. J., 3rd Cell-mediated immunity and immunosuppression in herpes simplex virus infection. Immunodeficiency. 1993;5(1):33–90. [PubMed] [Google Scholar]
  24. Stokes A., Corteyn A. H., Pullen L. A., Doel T. R., Meredith D. M., Killington R. A., Halliburton I. W., Whittaker G. R., Wheldon L. A., Nicolson L. Studies on glycoprotein 13 (gp13) of equid herpesvirus 1 using affinity-purified gp13, glycoprotein-specific monoclonal antibodies and synthetic peptides in a hamster model. J Gen Virol. 1991 Apr;72(Pt 4):923–931. doi: 10.1099/0022-1317-72-4-923. [DOI] [PubMed] [Google Scholar]
  25. Twardzik D. R., Mikovits J. A., Ranchalis J. E., Purchio A. F., Ellingsworth L., Ruscetti F. W. Gamma-interferon-induced activation of latent transforming growth factor-beta by human monocytes. Ann N Y Acad Sci. 1990;593:276–284. doi: 10.1111/j.1749-6632.1990.tb16119.x. [DOI] [PubMed] [Google Scholar]
  26. Woodroffe S. B., Garnett H. M., Danis V. A. Interleukin-1 production and cell-activation response to cytomegalovirus infection of vascular endothelial cells. Arch Virol. 1993;133(3-4):295–308. doi: 10.1007/BF01313770. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES