TABLE 4.

Synthetic effects of swi6 and mediator mutants grown at different temperatures

		Growth
25°	30°	37°	Suppression at HO-lacZ
	swi6:	+		++	++
Tail domain
SWI6 sin4	+	+	−
swi6 sin4	+/−	+/−	−	+
SWI6 pgd1	++	++	++
swi6 pgd1	(+)	+	(+)	+
SWI6 gal11	++	++	++
swi6 gal11	++	++	++	−
Middle domain
SWI6 nut1	++	++	++
swi6 nut1	++	++	++	+
SWI6 med1	+	++	++
swi6 med1	+	+	(+)	+
SWI6 med9	++	++	++
swi6 med9	(+)	(+)	(+)	+
SWI6 srb7	++	++	++
swi6 srb7	++	++	(+)	+
Kinase complex
SWI6 srb8	++	++	++
swi6 srb8	++	++	+	+
SWI6 srb9	++	++	++
swi6 srb9	++	++	+	+
SWI6 srb10	++	++	++
swi6 srb10	++	++	++	+
SWI6 srb11	++	++	++
swi6 srb11	++	++	+	+
Head domain
SWI6 srb2	++	++	++
swi6 srb2	+/−	+/−	+/−	ND
SWI6 srb5	+	+	+
swi6 srb5	+/−	+/−	+/−	ND
SWI6 rox3	++	++	++
swi6 rox3		++	++	++	+

The growth phenotypes of mediator subunit mutants in a swi6 background grown on YEPD plates were scored from best to worst as follows: ++, +, (+), +/−, −. ND, not determined. The suppression phenotype of each double mutant is indicated as “+” if the mediator defect restores HO-lacZ expression on the basis of the X-Gal filter assay described in materials and methods.