Abstract
Human lymphocytes from peripheral blood, bone marrow spleen and lymph nodes were cultured. Continuous phytoheamagglutinin (PHA) stimulation was used, first during a 24 h liquid preincubation, then during a 5 day culture in methylcellulose. In normal donors a rapid colony formation took place, with a mean of 124+/-82 colonies per 1 times 10(5) preincubated lymphocytes. Cells from such colonies were studied by cytology, scanning electron microscopy and rosette formation techniques; arguments favour the hypothesis that these could be T lymphocytes. Neither granulocytes nor macrophages could be grown, and no lymphoid colony formation occurred without PHA stimulation. The same technique was applied to patients with various lymphoproliferative disorders. Significant colony suppression was observed in nearly every case of chronic lymphatic leukaemia; the number of colonies was reduced in some patients with acute lymphatic leukaemia, lymphosarcoma, dysglobulinaemia and Hodgkin's disease. This lymphoid culture method should be applied to a larger number of patients to determine whether it has a classification value and/or prognostic significance. When colonies were grown in pathological states, rosette formation was identical to that of normal donors; colony formation could be due to persisting normal lymphocytes.
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- Bach J. F. Evaluation of T-cells and thymic serum factors in man using the rosette technique. Transplant Rev. 1973;16(0):196–217. doi: 10.1111/j.1600-065x.1973.tb00121.x. [DOI] [PubMed] [Google Scholar]
- Fibach E., Gerassi E., Sachs L. Induction of colony formation in vitro by human lymphocytes. Nature. 1976 Jan 15;259(5539):127–129. doi: 10.1038/259127a0. [DOI] [PubMed] [Google Scholar]
- Gerassi E., Sachs L. Regulation of the induction of colonies in vitro by normal human lymphocytes. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4546–4550. doi: 10.1073/pnas.73.12.4546. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kondracki E., Milgrom F. Direct and cooperative mechanisms of lymphocyte triggering in liquid and solid cultures. J Immunol. 1977 Feb;118(2):381–387. [PubMed] [Google Scholar]
- Metcalf D., Nossal G. J., Warner N. L., Miller J. F., Mandel T. E., Layton J. E., Gutman G. A. Growth of B-lymphocyte colonies in vitro. J Exp Med. 1975 Dec 1;142(6):1534–1549. doi: 10.1084/jem.142.6.1534. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Metcalf D., Warner N. L., Nossal G. J., Miller J. F., Shortman K., Rabellino E. Growth of B lymphocyte colonies in vitro from mouse lymphoid organs. Nature. 1975 Jun 19;255(5510):630–632. doi: 10.1038/255630a0. [DOI] [PubMed] [Google Scholar]
- Metcalf D., Wilson J. W., Shortman K., Miller J. F., Stocker J. The nature of the cells generating B-lymphocyte colonies in vitro. J Cell Physiol. 1976 May;88(1):107–116. doi: 10.1002/jcp.1040880112. [DOI] [PubMed] [Google Scholar]
- Rozenszajn L. A., Michlin H., Kalechman Y., Sredni B. Colony growth in vitro of mitogen-stimulated mouse B lymphocytes. Immunology. 1977 Mar;32(3):319–326. [PMC free article] [PubMed] [Google Scholar]
- Rozenszajn L. A., Shoham D., Kalechman I. Clonal proliferation of PHA-stimulated human lymphocytes in soft agar culture. Immunology. 1975 Dec;29(6):1041–1055. [PMC free article] [PubMed] [Google Scholar]
- Sredni B., Kalechman Y., Michlin H., Rozenszajn L. A. Development of colonies in vitro of mitogen-stimulated mouse T lymphocytes. Nature. 1976 Jan 15;259(5539):130–132. doi: 10.1038/259130a0. [DOI] [PubMed] [Google Scholar]
- Zeevi A., Goldman I., Rozenszajn L. A. Stimulation and inhibition of human T-lymphocyte colony cell proliferation by hemopoietic cell factors. Cell Immunol. 1977 Feb;28(2):235–247. doi: 10.1016/0008-8749(77)90108-3. [DOI] [PubMed] [Google Scholar]