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. 1978 Jul;35(1):77–84.

Polyadenylic acid-polyuridylic acid (poly A:U) and experimental murine brucellosis

II. Macrophages as target cells of poly A:U in experimental brucellosis*

Eloisa D Madraso, Christina Cheers
PMCID: PMC1457232  PMID: 680804

Abstract

Results of in vivo and in vitro experiments suggest that the early suppression of brucella growth in double stranded polyadenylic-polyuridylic acid-(poly A:U-) treated mice was due to a non-specific activation of macrophages by poly A:U. Poly A:U administered intraperitoneally at the time of brucella infection failed to enhance T-cell mediated responses to the organism, namely delayed-type hyper-sensitivity to brucellin and adoptive transfer of immunity to the infection. Poly A:U did not augment the protective antibodies formed in response to infection. Although poly A:U has been previously found to suppress brucellosis in athymic (nude) mice, it did not enhance the thymus-dependent antibody response to sheep erythrocytes in brucella-infected nudes, suggesting that it did not significantly enhance maturation of their helper T-cell percursors.

Increased macrophage spreading, an indication of activation, was seen immediately after administration of poly A:U and Brucella abortus. Later on the infection spreading was suppressed, a phenomenon which appears to relate to the biphasic effect of poly A:U in vivo described in the accompanying paper. Peritoneal macrophages treated in vitro with poly A:U were stimulated to spread on plastic surfaces, even when T lymphocytes were removed with anti-Thy-1 serum and complement.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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