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. 1979 Feb;36(2):331–338.

Estimation of the intravascular half-lives of normal rhesus monkey IgG, IgA and IgM.

S J Challacombe, M W Russell
PMCID: PMC1457453  PMID: 108203

Abstract

Rhesus monkey IgG, IgA and IgM were purified from pooled normal serum and labelled with 125I. Four monkeys were injected intravenously with approximately 50 microgram of each purified immunoglobulin containing between 25 muCi and 50 muCi of 125I. Iodination was approximately 1 atom per 14 molecules of IgG and IgA and per 2.5 molecules of IgM. Samples of serum were taken for up to 22 days after injection and radioactivity compared with that in a sample taken within 30 min of injection. Sucrose density ultracentrifugation of serum samples revealed that in animals given IgG or IgA, radioactivity was associated with 7S protein peaks, and in animals given IgM radioactivity was associated predominantly with a 19S protein peak. Most of the radioactivity could be precipitated from serum samples with the appropriate monospecific antiserum. The mean half-life of rhesus monkey IgG was estimated as 8.3 days, IgA as 4.5 days and IgM as 4.7 days.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERSON S. A., YALOW R. S. Chemical and biological alterations induced by irradiation of I 131 labeled human serum albumin. J Clin Invest. 1957 Jan;36(1 Pt 1):44–50. doi: 10.1172/JCI103408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BLOOM H. J., CROCKETT D. J., STEWART F. S. The effects of radiation on the stability of radio-iodinated human serum albumin. Br J Radiol. 1958 Jul;31(367):377–383. doi: 10.1259/0007-1285-31-367-377. [DOI] [PubMed] [Google Scholar]
  3. COHEN S., FREEMAN T., McFARLANE A. S. Metabolism of I-131-labelled human albumin. Clin Sci. 1961 Apr;20:161–170. [PubMed] [Google Scholar]
  4. COHEN S., HOLLOWAY R. C., MATTHEWS C., MCFARLANE A. S. Distribution and elimination of 131 I- and 14C-labelled plasma proteins in the rabbit. Biochem J. 1956 Jan;62(1):143–154. doi: 10.1042/bj0620143. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Challacombe S. J., Russell M. W., Hawkes J. E., Bergmeier L. A., Lehner T. Passage of immunoglobulins from plasma to the oral cavity in rhesus monkeys. Immunology. 1978 Dec;35(6):923–931. [PMC free article] [PubMed] [Google Scholar]
  6. Cohen S., Butcher G. A., Mitchell G. H., Deans J. A., Langhorne J. Acquired immunity and vaccination in malaria. Am J Trop Med Hyg. 1977 Nov;26(6 Pt 2):223–232. doi: 10.4269/ajtmh.1977.26.223. [DOI] [PubMed] [Google Scholar]
  7. DIXON F. J., TALMAGE D. W., MAURER P. H., DEICHMILLER M. The half-life on homologous gamma globulin (antibody) in several species. J Exp Med. 1952 May;95(5):313–318. doi: 10.1084/jem.96.4.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FAHEY J. L., SELL S. THE IMMUNOGLOBULINS OF MICE. V. THE METABOLIC (CATABOLIC) PROPERTIES OF FIVE IMMUNOGLOBULIN CLASSES. J Exp Med. 1965 Jul 1;122:41–58. doi: 10.1084/jem.122.1.41. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. FREEMAN T. The biological behaviour of normal and denatured human plasma albumin. Clin Chim Acta. 1959 Nov;4:788–792. doi: 10.1016/0009-8981(59)90029-4. [DOI] [PubMed] [Google Scholar]
  10. Lehner T., Challacombe S. J., Caldwell J. An experimental model for immunological studies of dental caries in the rhesus monkey. Arch Oral Biol. 1975 May-Jun;20(5-6):299–304. doi: 10.1016/0003-9969(75)90018-7. [DOI] [PubMed] [Google Scholar]
  11. Lehner T., Challacombe S. J., Caldwell J. Immunologic basis for vaccination against dental caries in rhesus monkeys. J Dent Res. 1976 Apr;55(Spec No):C166–C180. doi: 10.1177/002203457605500311011. [DOI] [PubMed] [Google Scholar]
  12. McFARLANE A. S. The behavior of I 131-labeled plasma proteins in vivo. Ann N Y Acad Sci. 1957 Aug 30;70(1):19–25. doi: 10.1111/j.1749-6632.1957.tb35374.x. [DOI] [PubMed] [Google Scholar]
  13. McFarlane A. S. IN VIVO BEHAVIOR OF I-FIBRINOGEN. J Clin Invest. 1963 Mar;42(3):346–361. doi: 10.1172/JCI104721. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Monte-Wicher V., Wicher K., Arbesman C. E. Comparative studies of monkey and human immunoglobulins. Immunochemistry. 1970 Oct;7(10):839–849. doi: 10.1016/0019-2791(70)90060-1. [DOI] [PubMed] [Google Scholar]
  15. Rogentine G. N., Jr, Vaal L., Ellis E. B., Darrow C. C., 2nd Rhesus lymphocyte alloantigens. I. Identification of a major alloantigen system. Transplantation. 1971 Oct;12(4):267–270. [PubMed] [Google Scholar]
  16. STERLING K. The turnover rate of serum albumin in man as measured by I131-tagged albumin. J Clin Invest. 1951 Nov;30(11):1228–1237. doi: 10.1172/JCI102542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Schenkel R. H., Simpson G. L., Silverman P. H. Vaccination of Rhesus monkeys (Macaca mulatta) against Plasmodium knowlesi by the use of nonviable antigen. Bull World Health Organ. 1973 May;48(5):597–604. [PMC free article] [PubMed] [Google Scholar]
  18. Strober W., Wochner R. D., Barlow M. H., McFarlin D. E., Waldmann T. A. Immunoglobulin metabolism in ataxia telangiectasia. J Clin Invest. 1968 Aug;47(8):1905–1915. doi: 10.1172/JCI105881. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Van Rood J. J., Balner R., Gabb B. W., Dersjant H., Van Vreeswijk W. Major histocompatibility locus of rhesus monkeys (RhL-A). Nat New Biol. 1971 Apr 7;230(14):177–180. doi: 10.1038/newbio230177a0. [DOI] [PubMed] [Google Scholar]

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