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. 1978 Nov;35(5):763–769.

Specificity and genetic restrictions of the guinea-pig immune response to dinitrophenyl-lysyl-alanyl octapeptides.

A Campos-Neto, S F Schlossman, H Levine, A Yanovski, A Yaron
PMCID: PMC1457536  PMID: 102589

Abstract

A series of 2,4-dinitrophenyl (DNP) octapeptides containing L-lysine and L-alanine were prepared to examine the specificity and genetic restrictions of both cellular and humoral immune responses in inbred guinea-pigs. Strains 2 and 13 guinea-pigs were therefore immunized with Lys4-Ala3-Lys(DNP), Lys3Ala4-Lys(DNP), Lys2-Ala5-Lys(DNP) and Lys-Ala6-Lys(DNP). Only Lys4-Ala3-Lys(DNP) was under Ir gene control and could induce both antibody and T-cell responses in strain 2 guinea-pigs. In contrast, Lys4-Ala3-Lys(DNP) injected in strain 13 guinea-pigs and the other DNP-octapeptides injected in strain 2 or 13 guinea-pigs elicited only antibody formation and no specific T-cell mediated response. Antibody formed in the absence of specific T-cell responsiveness in either strain 2 or 13 was hapten specific and lacked the capacity to discriminate the immunizing antigen from closely related DNP-peptides. Antibody produced by animals with specific T-cell responses, on the other hand, was exquisitely specific for the immunizing peptide and could discriminate it from closely related peptides.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Civin C. I., Levine H. B., Williamson A. R., Schlossman S. F. The effects of antigen dose and adjuvant on the antibody response; amplification of restricted B cell clones. J Immunol. 1976 May;116(5):1400–1406. [PubMed] [Google Scholar]
  2. Gershon R. K., Paul W. E. Effect of thymus-derived lymphocytes on amount and affinity of anti-hapten antibody. J Immunol. 1971 Mar;106(3):872–874. [PubMed] [Google Scholar]
  3. Inman J. K., Merchant B., Claflin L., Tacey S. E. Coupling of large haptens to proteins and cell surfaces: preparation of stable, optimally sensitized erythrocytes for hapten-specific, hemolytic plaque assays. Immunochemistry. 1973 Mar;10(3):165–174. doi: 10.1016/0019-2791(73)90005-0. [DOI] [PubMed] [Google Scholar]
  4. Kalir R., Fridkin M., Patchornik A. (4-Hydroxy-3-nitro)benzylated polystyrene. An improved polymeric nitrophenol derivative for peptide synthesis. Eur J Biochem. 1974 Feb 15;42(1):151–156. doi: 10.1111/j.1432-1033.1974.tb03324.x. [DOI] [PubMed] [Google Scholar]
  5. LEVINE B. B., OJEDA A., BENACERRAF B. STUDIES ON ARTIFICIAL ANTIGENS. III. THE GENETIC CONTROL OF THE IMMUNE RESPONSE TO HAPTEN-POLY-L-LYSINE CONJUGATES IN GUINEA PIGS. J Exp Med. 1963 Dec 1;118:953–957. doi: 10.1084/jem.118.6.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Levin H. A., Levine H., Schlossman S. F. Antigen recognition and antibody specificity. Carrier specificity and genetic control of anti-dinitrophenyl-oligolysine antibody. J Exp Med. 1971 Jun 1;133(6):1199–1218. doi: 10.1084/jem.133.6.1199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Levin H. A., Levine H., Schlossman S. F. Studies on the specificity and affinity of alpha, DNP-oligolysine antibody: a basis for questioning the role of cell-bound antibody in cellular recognition of antigen. J Immunol. 1970 Jun;104(6):1377–1383. [PubMed] [Google Scholar]
  8. Schlossman S. F. Antigen recognition: the specificity of T cells involved in the cellular immune response. Transplant Rev. 1972;10:97–111. doi: 10.1111/j.1600-065x.1972.tb01540.x. [DOI] [PubMed] [Google Scholar]
  9. Schlossman S. F., Herman J., Yaron A. Antigen recognition: in vitro studies on the specificity of the cellular immune response. J Exp Med. 1969 Nov 1;130(5):1031–1045. doi: 10.1084/jem.130.5.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schlossman S. F., Yaron A., Ben-Efraim S., Sober H. A. Immunogenicity of a series of alpha,N-DNP-L-lysines. Biochemistry. 1965 Aug;4(8):1638–1645. doi: 10.1021/bi00884a028. [DOI] [PubMed] [Google Scholar]
  11. Schlossman S. F., Yaron A. Immunochemical studies on the specificity of cellular and antibody-mediated immune reactions. Ann N Y Acad Sci. 1970 Feb 13;169(1):108–115. doi: 10.1111/j.1749-6632.1970.tb55975.x. [DOI] [PubMed] [Google Scholar]
  12. Stashenko P. P., Schlossman S. F. Antigen recognition: the specificity of an isolated T lymphocyte population. J Immunol. 1977 Feb;118(2):544–550. [PubMed] [Google Scholar]
  13. Stupp Y., Paul W. E., Benacerraf B. Structural control of immunogenicity. II. Antibody synthesis and cellular immunity in response to immunization with mono-epsilon-oligo-L-lysines. Immunology. 1971 Oct;21(4):583–594. [PMC free article] [PubMed] [Google Scholar]
  14. Yaron A., Dunham E. K., 3rd, Schlossman S. F. Synthesis and immunological properties of the oligolysyl-N-epsilon-dinitrophenyllysine and oligolysylalanylalanylalanyl-N-epsilon-dinitrophenyllysine peptide series. Biochemistry. 1974 Jan 15;13(2):347–354. doi: 10.1021/bi00699a020. [DOI] [PubMed] [Google Scholar]
  15. Yaron A., Schlossman S. F. Preparation and immunologic properties of stereospecific alpha-dinitrophenylnonalysines. Biochemistry. 1968 Jul;7(7):2673–2681. doi: 10.1021/bi00847a034. [DOI] [PubMed] [Google Scholar]
  16. von Boehmer H., Shortman K. The separation of different cell classes from lymphoid organs. IX. A simple and rapid method for removal of damaged cells from lymphoid cell suspensions. J Immunol Methods. 1973 Apr;2(3):293–301. doi: 10.1016/0022-1759(73)90055-0. [DOI] [PubMed] [Google Scholar]

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