Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1979 Mar;36(3):549–555.

Comparison of the direct antiglobulin rosetting reaction (DARR) and direct immunofluorescence (DIF) for demonstration of sIg-bearing lymphocytes in pigs, sheep and cattle.

R M Binns, S T Licence, D B Symons, B W Gurner, R R Coombs, D E Walters
PMCID: PMC1457560  PMID: 374254

Abstract

Tests with untreated and trypsin-treated red cells (rbc) from a variety of species showed that anti-Ig-coupled pig RBC are good indicator cells for the study of ruminant blood sIg + lymphocytes by the DARR test; coupled donkey and rabbit RBC are suitable for investigating pig lymphocytes. The different species showed the following percentages of sIg + lymphocytes (M +/- SE) by direct immunofluorescence (DIF) and the direct antiglobulin rosetting reaction (DARR) respectively:pigs 9.2 +/- 0.7% and 16.3 +/- 1.2%; sheep 20.2 +/- 1.2% and 33.1 +/- 1.6%; Cattle 13.5 +/- 1.4% and 28.9 +/- 3.5%. The mean ratio of sIg + lymphocytes shown by the two tests (DARR/DIF) for each species was 1.80 +/- 0.08 for pigs, 1.73 +/- 0.7 for sheep and 2.15 +/- 0.18 for cattle. Preincubation of pig and sheep lymphocytes at 37 degrees for 1 h did not alter the proportion of sIg + lymphocytes detected by either test. Thus the DARR test reveals a further population of sIg + lymphocytes in addition to that detected by immunofluorescence, whose number is proportional to the B population as measured by DIF and whose sIg is intimately associated with the membrane.

Full text

PDF
549

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binns R. M., Pallares V., Symons D. B., Sibbons P. Effect of thymectomy on lymphocyte subpopulations in the pig. Demonstration of a thymus-dependent 'null' cell. Int Arch Allergy Appl Immunol. 1977;55(1-6):96–101. doi: 10.1159/000231915. [DOI] [PubMed] [Google Scholar]
  2. Binns R. M. Sheep erythrocyte rosettes in pigs, sheep, cattle and goats demonstrated in the presence of dextran. J Immunol Methods. 1978;21(3-4):197–210. doi: 10.1016/0022-1759(78)90146-1. [DOI] [PubMed] [Google Scholar]
  3. Binns R. M., Symons D. B. The ontogeny of lymphocyte immunoglobin determinants and their relationship to antibody production in the fetal pig. Res Vet Sci. 1974 Mar;16(2):260–262. [PubMed] [Google Scholar]
  4. Coombs R. R., Wilson A. B., Eremin O., Gurner B. W., Haegert D. G., Lawson Y. A., Bright S., Munro A. J. Comparison of the direct antiglobulin rosetting reaction with the mixed antiglobulin rosetting reaction for the detection of immunoglobulin on lymphocytes. J Immunol Methods. 1977;18(1-2):45–54. doi: 10.1016/0022-1759(77)90157-0. [DOI] [PubMed] [Google Scholar]
  5. Escajadillo C., Binns R. M. Rosette formation with sheep erythrocytes--a possible T-cell marker in the pig. Int Arch Allergy Appl Immunol. 1975;48(2):261–275. doi: 10.1159/000231312. [DOI] [PubMed] [Google Scholar]
  6. Haegert D. G., Coombs R. R. Immunoglobulin-positive mononuclear cells in human peripheral blood: detection by mixed anti-globulin and direct anti-globulin-rosetting reactions. J Immunol. 1976 May;116(5):1426–1430. [PubMed] [Google Scholar]
  7. Symons D. B., Binns R. M. Immunoglobulin-bearing lymphocytes: their demonstration in adult sheep and ontogeny in the sheep fetus. Int Arch Allergy Appl Immunol. 1975;49(5):658–669. doi: 10.1159/000231448. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES