Abstract
A new quantitative method for the measurement of macrophage aggregation, induced by lymphokine preparations, is described. This involves the continuous measurement of the light absorbance of stirred macrophage suspensions. The results have been compared with those obtained by measuring macrophage migration inhibition activity for the detection of lymphokine activity. Separation of crude lymphocyte culture supernatants by Sephadex G-200 shows that the material with aggregating activity is heterogeneous. The greater amount of this activity is not separated from macrophage migration inhibition activity, and has a molecular weight of 35,000--70,000. A comparison has been made with other published methods for measuring macrophage aggregation.
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- Dumonde D. C., Page D. A., Matthew M., Wolstencroft R. A. Role of lymphocyte activation products (LAP) in cell-mediated immunity. I. Preparation and partial purification of guinea-pig LAP. Clin Exp Immunol. 1972 Jan;10(1):25–47. [PMC free article] [PubMed] [Google Scholar]
- Fox R. A., Gregory D. S., Feldman J. D. Macrophage receptors for migration inhibitory factor (MIF), migration stimulatory factor (MSF), and agglutinating factor. J Immunol. 1974 May;112(5):1867–1872. [PubMed] [Google Scholar]
- GEORGE M., VAUGHAN J. H. In vitro cell migration as a model for delayed hypersensitivity. Proc Soc Exp Biol Med. 1962 Nov;111:514–521. doi: 10.3181/00379727-111-27841. [DOI] [PubMed] [Google Scholar]
- Garcia-Moreno L. F., Myrvik Q. N. Macrophage-agglutinating factor produced in vitro by BCG-sensitized lymphocytes. Infect Immun. 1977 Sep;17(3):613–620. doi: 10.1128/iai.17.3.613-620.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lolekha S., Dray S., Gotoff S. P. Macrophage aggregation in vitro: a correlate of delayed hypersensitivity. J Immunol. 1970 Feb;104(2):296–304. [PubMed] [Google Scholar]
- Postlethwaite A. E., Kang A. H. Guinea pig lymphocyte-derived macrophage aggregation factor: its separation from macrophage migration inhibitory factor. J Immunol. 1976 Nov;117(5 Pt 1):1651–1655. [PubMed] [Google Scholar]