Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1978 Mar;34(3):431–437.

Active suppression of in vitro reactivity of spleen cells after BCG treatment.

S Orbach-Arbouys, M F Poupon
PMCID: PMC1457593  PMID: 305890

Abstract

It was found that spleen cells from mice injected i.v. with large doses of BCG respond to PHA stimulation less intensely than do normal spleen cells. We were able to show that nylon wool column purified BCG treated T cells also had a low PHA reactivity. Unfractionated spleen cells, adherent cells or T-enriched populations from BCG treated mice, when added to normal T cells lowered their PHA reactivity. When the same BCG treated cell populations were added to tumor cells in vitro, they inhibited their growth.

Full text

PDF
431

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baird L. G., Kaplan A. M. Macrophage regulation of mitogen-induced blastogenesis. I. Demonstration of inhibitory cells in the spleens and peritoneal exudates of mice. Cell Immunol. 1977 Jan;28(1):22–35. doi: 10.1016/s0008-8749(77)80003-8. [DOI] [PubMed] [Google Scholar]
  2. Clinton B. A., Magoc T. J., Aspinall R. L., Rapoza N. P. The influence upon mitogenic and cellular immunologic reactive systems in vitro by poly(I:C) and BCG murine interferons induced in vivo. Cell Immunol. 1976 Nov;27(1):60–70. doi: 10.1016/0008-8749(76)90154-4. [DOI] [PubMed] [Google Scholar]
  3. Florentin I., Orbach-Arbouys S. Réactivité des cellules spléniques après traitement par le BCG: variation de la réponse selon les tests et les legnées utilisées. Ann Immunol (Paris) 1977 Jan-Mar;128(1-2):271–272. [PubMed] [Google Scholar]
  4. Germain R. N., Williams R. M., Benacerraf B. Specific and nonspecific antitumor immunity. II. Macrophage-mediated nonspecific effector activity induced by BCG and similar agents. J Natl Cancer Inst. 1975 Mar;54(3):709–720. [PubMed] [Google Scholar]
  5. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  6. Kirchner H., Holden H. T., Herberman Splenic suppressor macrophages induced in mice by injection of Corynebacterium parvum. J Immunol. 1975 Nov;115(5):1212–1216. [PubMed] [Google Scholar]
  7. Lespinats G., Poupon M. F. Cytostatic effect of spleen cells of tumor-bearing mice on syngenetic tumor cells. Cancer Res. 1977 Jun;37(6):1727–1732. [PubMed] [Google Scholar]
  8. Mackaness G. B., Lagrange P. H., Ishibashi T. The modifying effect of BCG on the immunological induction of T cells. J Exp Med. 1974 Jun 1;139(6):1540–1552. doi: 10.1084/jem.139.6.1540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mathé G., Amiel J. L., Schwarzenberg L., Schneider M., Cattan A., Schlumberger J. R., Hayat M., De Vassal F. Active immunotherapy for acute lymphoblastic leukaemia. Lancet. 1969 Apr 5;1(7597):697–699. doi: 10.1016/s0140-6736(69)92648-8. [DOI] [PubMed] [Google Scholar]
  10. Miller T. E., Mackaness G. B., Lagrange P. H. Immunopotentiation with BCG. II. Modulation of the response to sheep red blood cells. J Natl Cancer Inst. 1973 Nov;51(5):1669–1676. doi: 10.1093/jnci/51.5.1669. [DOI] [PubMed] [Google Scholar]
  11. Mitchell M. S., Kirkpatrick D., Mokyr M. B., Gery I. On the mode of action of BCG. Nat New Biol. 1973 Jun 13;243(128):216–218. doi: 10.1038/newbio243216a0. [DOI] [PubMed] [Google Scholar]
  12. Williams R. M., Germain R. N., Benacerraf B. Specific and nonspecific antitumor immunity. I. Description of an in vitro assay based on inhibition of DNA synthesis in tumor cells. J Natl Cancer Inst. 1975 Mar;54(3):697–708. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES