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. 1978 Feb;34(2):199–206.

Further studies of the adjuvant properties of homologous IgG split products: mode of action of F(ab')2 and related fragments.

A J Kulberg, L M Bartova, D N Evnin
PMCID: PMC1457697  PMID: 342398

Abstract

Peptic bivalent and monovalent Fab' fragments as well as F(ab')2 fragment from a purified rabbit anti-DNP antibody were equally effective in enhancement of the immune response to SRBC. In contrast, the papain Fab fragment of normal IgG possessed a considerably lower enhancing activity. These data indicate that the effector site(s) controlling the adjuvant activity of F(ab')2 and related fragments is structurally distinct from the antibody active site(s) within the fragment molecule, and is located in C-terminal region of the heavy chain Fd fragment. When F(ab')2 fragment was injected into rabbits together with the proteinase inhibitor Trasylol it failed to enhance the immune response to SRBC. Furthermore, Trasylol interferes with the ability of F(ab')2 or Fab' fragment to activate the complement system both in vivo and in vitro. This, together with the observation that F(ab')2 fragments enhance the response to T-dependent antigens (SRBC and bovine gamma globulin) but fail to augment the response to LPS, suggests that these fragments may operate via activation of complement and thereby influence indirectly the reactivity of complement receptor B cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartova L. M., Shmeleva N. E., Tarkhanova I. A. Complement - fixation in the interaction of normal rabbit gamma G' globulin and its Fab' fragment. J Hyg Epidemiol Microbiol Immunol. 1976;20(1):121–127. [PubMed] [Google Scholar]
  2. Fairclough G. F., Jr, Fruton J. S. Peptide-protein interaction as studied by gel filtration. Biochemistry. 1966 Feb;5(2):673–683. doi: 10.1021/bi00866a038. [DOI] [PubMed] [Google Scholar]
  3. Fearon D. T., Austen K. F., Ruddy S. Properdin factor D: characterization of its active site and isolation of the precursor form. J Exp Med. 1974 Feb 1;139(2):355–366. doi: 10.1084/jem.139.2.355. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Givol D., De Lorenzo F. The position of various cleavages of rabbit immunoglobulin G. J Biol Chem. 1968 Apr 25;243(8):1886–1891. [PubMed] [Google Scholar]
  5. HARBOE M., RAU B., AHO K. PROPERTIES OF VARIOUS ANTI-GAMMA-GLOBULIN FACTORS IN HUMAN SERA. J Exp Med. 1965 Apr 1;121:503–519. doi: 10.1084/jem.121.4.503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kormeier L. C., Ing J. T., Mandy W. J. Specificity of antiglobulin factors in normal human serum reacting with enzyme digested gamma-G-globulin. J Immunol. 1968 Mar;100(3):612–621. [PubMed] [Google Scholar]
  7. Kulberg A. I., Bartova L. M., Shmelieva N. E. Interaction of anti-DNP rabbit antibody with peptid Fab' fragment of homologous IgG. Immunochemistry. 1971 Aug;8(8):733–739. doi: 10.1016/0019-2791(71)90049-8. [DOI] [PubMed] [Google Scholar]
  8. Kulberg A. I. Effector functions of immunoglobulins, molecular mechanisms. Allerg Immunol (Leipz) 1972;18(4):217–240. [PubMed] [Google Scholar]
  9. Kulberg A. J., Evnin D. N., Tarkhanova I. A. Enhancement of the immune response by a catabolic product of normal rabbit IgG. Effect of F(ab')2-like fragment. Immunology. 1976 May;30(5):715–722. [PMC free article] [PubMed] [Google Scholar]
  10. Lewis G. K., Ranken R., Nitecki D. E., Goodman J. W. Murine B-cell subpopulations responsive to T-dependent and T-independent antigens. J Exp Med. 1976 Aug 1;144(2):382–397. doi: 10.1084/jem.144.2.382. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mandy W. J. A new serum factor in normal rabbits. 3. Specificity for antigenic determinants uncovered by papain or pepsin digestion. J Immunol. 1967 Oct;99(4):815–824. [PubMed] [Google Scholar]
  12. Mellbye O. J., Natvig J. B. Evidence for immune complexes containing antibody to the pepsin site of IgG in rheumatoid synovial fluids. Clin Exp Immunol. 1971 Jun;8(6):889–899. [PMC free article] [PubMed] [Google Scholar]
  13. NISONOFF A., WISSLER F. C., LIPMAN L. N., WOERNLEY D. L. Separation of univalent fragments from the bivalent rabbit antibody molecule by reduction of disulfide bonds. Arch Biochem Biophys. 1960 Aug;89:230–244. doi: 10.1016/0003-9861(60)90049-7. [DOI] [PubMed] [Google Scholar]
  14. PORTER R. R. The hydrolysis of rabbit y-globulin and antibodies with crystalline papain. Biochem J. 1959 Sep;73:119–126. doi: 10.1042/bj0730119. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pepys M. B. Role of complement in induction of antibody production in vivo. Effect of cobra factor and other C3-reactive agents on thymus-dependent and thymus-independent antibody responses. J Exp Med. 1974 Jul 1;140(1):126–145. doi: 10.1084/jem.140.1.126. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Richie E. R., Grote D., Mandy W. J. A new serum factor in normal rabbits. 8. Isolation and characterization of a buried antigenic IgG determinant. J Immunol. 1972 Jun;108(6):1482–1490. [PubMed] [Google Scholar]
  17. Richie E. R., Woolsey M. E., Mandy W. J. A new serum factor in normal rabbits. VI. Reaction with buried determinants of IgG exposed sequentially with CNBr, pepsin and papain. J Immunol. 1970 Apr;104(4):984–991. [PubMed] [Google Scholar]
  18. Sandberg A. L., Oliveira B., Osler A. G. Two complement interaction sites in guinea pig immunoglobulins. J Immunol. 1971 Jan;106(1):282–285. [PubMed] [Google Scholar]
  19. Watanabe S., Kitagawa M. Naturally occurring rabbit antibodies against the tryptic 3-5S and 5S fragments of rabbit IgG--II. Immunochemistry. 1970 Apr;7(4):363–372. doi: 10.1016/0019-2791(70)90239-9. [DOI] [PubMed] [Google Scholar]

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