Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1978 Feb;34(2):315–321.

Shedding and reappearance of Fc, C3 and SRBC receptors on peripheral lymphocytes from normal donors and chronic lymphatic leukaemia (CLL) patients.

G Sármay, L István, J Gergely
PMCID: PMC1457710  PMID: 627410

Abstract

The shedding and resynthesis of Fc, C3 receptors, and receptors binding SRBC has been studied in normal peripheral lymphocytes, in lymphocytes from CLL patients and in a Raji cell line during 4 h incubation at 37 degrees. During the first part of the incubation, an increased release of receptors was observed. The shed, functionally active, receptors were detectable in the incubating medium on the basis of their ability to inhibit rosette formation specifically. After 4 h incubation the original ratios of the different rosette forming cells were observed, showing that the cells were capable of resynthesizing their shed receptors. This later phenomenon may be the result of an active metabolic process. The shedding was not detectable on Raji cells. Different patterns of receptor shedding and resynthesis were demonstrated by the lymphocytes of CLL patients. The correlations between the reappearance of the receptors on the cells, and the metabolism of the lymphocytes, is discussed.

Full text

PDF
315

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Basten A., Miller J. F., Sprent J., Pye J. A receptor for antibody on B lymphocytes. I. Method of detection and functional significance. J Exp Med. 1972 Mar 1;135(3):610–626. doi: 10.1084/jem.135.3.610. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Basten A., Warner N. L., Mandel T. A receptor for antibody on B lymphocytes. II. Immunochemical and electron microscopy characteristics. J Exp Med. 1972 Mar 1;135(3):627–642. doi: 10.1084/jem.135.3.627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bouroncle B. A., Clausen K. P., Aschenbrand J. F. Studies of the delayed response of phytohemagglutinin (PHA) stimulated lymphocytes in 25 chronic lymphatic leukemia patients before and during therapy. Blood. 1969 Aug;34(2):166–178. [PubMed] [Google Scholar]
  5. Brain P., Gordon J., Willetts W. A. Rosette formation by peripheral lymphocytes. Clin Exp Immunol. 1970 May;6(5):681–688. [PMC free article] [PubMed] [Google Scholar]
  6. Brody J. I., Oski F. A., Singer D. E. Impaired pentose phosphate shunt and decreased glycolytic activity in lymphocytes of chronic lymphocytic leukemia. Metabolic pathway. Blood. 1969 Oct;34(4):421–429. [PubMed] [Google Scholar]
  7. Cone R. E., Marchalonis J. J., Rolley R. T. Lymphocyte membrane dynamics. Metabolic release of cell surface proteins. J Exp Med. 1971 Dec 1;134(6):1373–1384. doi: 10.1084/jem.134.6.1373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Dickler H. B., Kunkel H. G. Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972 Jul 1;136(1):191–196. doi: 10.1084/jem.136.1.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Froland S. S., Natvig J. B. Identification of three different human lymphocyte populations by surface markers. Transplant Rev. 1973;16:114–162. doi: 10.1111/j.1600-065x.1973.tb00119.x. [DOI] [PubMed] [Google Scholar]
  10. Froland S. S., Wisloff F., Michaelsen T. E. Human lymphocytes with receptors for IgG. A population of cells distinct from T- and B-lymphocytes. Int Arch Allergy Appl Immunol. 1974;47(1):124–138. doi: 10.1159/000231207. [DOI] [PubMed] [Google Scholar]
  11. Grey H. M., Rabellino E., Pirofsky B. Immunoglobulins on the surface of lymphocytes. IV. Distribution in hypogammaglobulinemia, cellular immune deficiency, and chronic lymphatic leukemia. J Clin Invest. 1971 Nov;50(11):2368–2375. doi: 10.1172/JCI106735. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Grundke-Iqbal I., Uhr J. W. Cell surface immunoglobulin. VI. Dynamics on a human lymphoma line. Eur J Immunol. 1974 Mar;4(3):159–163. doi: 10.1002/eji.1830040303. [DOI] [PubMed] [Google Scholar]
  13. Jondal M., Klein G., Oldstone M. B., Bokish V., Yefenof E. Surface markers on human B and T lymphocytes. VIII. Association between complement and Epstein-Barr virus receptors on human lymphoid cells. Scand J Immunol. 1976;5(4):401–410. doi: 10.1111/j.1365-3083.1976.tb00294.x. [DOI] [PubMed] [Google Scholar]
  14. Lay W. H., Mendes N. F., Bianco C., Nussenzweig V. Binding of sheep red blood cells to a large population of human lymphocytes. Nature. 1971 Apr 23;230(5295):531–532. doi: 10.1038/230531a0. [DOI] [PubMed] [Google Scholar]
  15. Loor F., Forni L., Pernis B. The dynamic state of the lymphocyte membrane. Factors affecting the distribution and turnover of surface immunoglobulins. Eur J Immunol. 1972 Jun;2(3):203–212. doi: 10.1002/eji.1830020304. [DOI] [PubMed] [Google Scholar]
  16. Mendes N. F., Miki S. S., Peixinho Z. F. Combined detection of human T and B lymphocytes by rosette formation with sheep erythrocytes and zymosan-C3 complexes. J Immunol. 1974 Aug;113(2):531–536. [PubMed] [Google Scholar]
  17. Papamichail M., Brown J. C., Holborow E. J. Immunoglobulins on the surface of human lymphocytes. Lancet. 1971 Oct 16;2(7729):850–852. doi: 10.1016/s0140-6736(71)90224-8. [DOI] [PubMed] [Google Scholar]
  18. Rabinowitz Y., Schimo I., Wilhite B. A. Metabolic responses to separated leucocytes to phytohaemagglutinin: effects of anaerobiasis, actinomycin D and puromycin. Br J Haematol. 1968 Nov;15(5):455–464. doi: 10.1111/j.1365-2141.1968.tb01565.x. [DOI] [PubMed] [Google Scholar]
  19. Sagone A. L., Jr, Murphy S. G. The chronic lymphatic leukemia lymphocyte: correlation of functional, metabolic, and surface immunoglobulin characteristics. Cell Immunol. 1975 Jul;18(1):1–8. doi: 10.1016/0008-8749(75)90030-1. [DOI] [PubMed] [Google Scholar]
  20. Singer S. J., Nicolson G. L. The fluid mosaic model of the structure of cell membranes. Science. 1972 Feb 18;175(4023):720–731. doi: 10.1126/science.175.4023.720. [DOI] [PubMed] [Google Scholar]
  21. Smith J. L., Cowling D. C., Barker C. R. Response of lymphocytes in chronic lymphocytic leukaemia to plant mitogens. Lancet. 1972 Jan 29;1(7744):229–233. doi: 10.1016/s0140-6736(72)90624-1. [DOI] [PubMed] [Google Scholar]
  22. Vitetta E. S., Uhr J. W. Cell surface immunoglobulin. V. Release from murine splenic lymphocytes. J Exp Med. 1972 Oct 1;136(4):676–696. doi: 10.1084/jem.136.4.676. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Wilson J. D., Nossal G. J., Lewis H. Metabolic characteristics of lymphocyte surface immunoglobulins. Eur J Immunol. 1972 Jun;2(3):225–232. doi: 10.1002/eji.1830020306. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES