Skip to main content
Immunology logoLink to Immunology
. 1979 Jul;37(3):563–568.

Time kinetics of the interaction of concanavalin A with splenic lymphocytes of normal AKR mice.

K B Sainis, A N Bhisey, K Sundaram, G P Phondke
PMCID: PMC1457718  PMID: 500116

Abstract

Interaction of concanavalin A (Con A) with splenic lymphocytes of normal AKR mice led to concentration-dependent biphasic changes in electrophoretic mobility. Kinetics of this interaction suggested that at high concentration Con A interacted in two steps. The first step led to redistribution of Con A receptors and increased EPM. In the second step additional receptors to Con A became accessible and their interaction with Con A reduced surface charge density. The kinetic data thus provide additional evidence for the existence of two sets of qualitatively different receptors for Con A on AKR lymphocytes.

Full text

PDF
563

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BANGHAM A. D., HEARD D. H., FLEMANS R., SEAMAN G. V. An apparatus for microelectrophoresis of small particles. Nature. 1958 Sep 6;182(4636):642–644. doi: 10.1038/182642a0. [DOI] [PubMed] [Google Scholar]
  2. Blume P., Malley A., Knox R. J., Seaman G. V. Electrophoretic mobility as a sensitive probe of lectin-lymphocyte interaction. Nature. 1978 Jan 26;271(5643):378–380. doi: 10.1038/271378a0. [DOI] [PubMed] [Google Scholar]
  3. Brown S. S., Revel J. P. Reversibility of cell surface label rearrangement. J Cell Biol. 1976 Mar;68(3):629–641. doi: 10.1083/jcb.68.3.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. De Petris S., Raff M. C., Mallucci L. Ligand-induced redistribution of concanavalin A receptors on normal, trypsinized and transformed fibroblasts. Nat New Biol. 1973 Aug 29;244(139):275–278. doi: 10.1038/newbio244275a0. [DOI] [PubMed] [Google Scholar]
  5. Sainis K. B., Bhisey A. N., Sundaram K., Phondke G. P. Cell electrophoretic studies on mouse lymphocytes treated with concanavalin A: possible existence of two types of receptors on cell surface. Immunology. 1979 Jul;37(3):555–561. [PMC free article] [PubMed] [Google Scholar]
  6. Shoham J., Sachs L. Differences in the binding of fluorescent concanavalin A to the surface membrane of normal and transformed cells. Proc Natl Acad Sci U S A. 1972 Sep;69(9):2479–2482. doi: 10.1073/pnas.69.9.2479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Wioland M., Donner M., Neauport-Sautes C. Modifications of the thymocyte membrane during redistribution of concanavalin A receptors. Eur J Immunol. 1976 Apr;6(4):273–278. doi: 10.1002/eji.1830060408. [DOI] [PubMed] [Google Scholar]
  8. Wioland M. Electrophoretic mobility of the thymocyte during endocytosis of concanavalin A. Cell Immunol. 1974 Jun;12(3):472–475. doi: 10.1016/0008-8749(74)90102-6. [DOI] [PubMed] [Google Scholar]
  9. Yahara I., Edelman G. M. Electron microscopic analysis of the modulation of lymphocyte receptor mobility. Exp Cell Res. 1975 Mar 1;91(1):125–142. doi: 10.1016/0014-4827(75)90150-0. [DOI] [PubMed] [Google Scholar]
  10. Yata J., Desgranges C., Tachibana T., de Thé G. Separation of human lymphocytes forming spontaneous rosettes with sheep erythrocytes. Biomedicine. 1973 Nov 20;19(11):475–478. [PubMed] [Google Scholar]
  11. de Petris S. Concanavalin A receptors, immunoglobulins, and theta antigen of the lymphocyte surface. Interactions with concanavalin A and with Cytoplasmic structures. J Cell Biol. 1975 Apr;65(1):123–146. doi: 10.1083/jcb.65.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES