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. 1979 Jul;37(3):643–651.

Kinetics of B-lymphocytes stimulation by pokeweed Pa-1 mitogen and bacterial lipopolysaccharide.

K Yokoyama, T Osawa
PMCID: PMC1457722  PMID: 315363

Abstract

The presence of two subpopulations in mouse B lymphocytes responding to pokeweed Pa-1 mitogen or bacterial lipopolysaccharide (LPS) in terms of DNA synthesis was demonstrated. The analysis of surface markers of these two subpopulations revealed that one of the subpopulations carried the complement receptor (CR+) and the other lacked the complement receptor (CR-). The kinetics of stimulation by Pa-1 and LPS differed between these two subpopulations. CR+ -B cells exhibited the first peak of DNA synthesis 27 h after the addition of the mitogen, whereas CR- -B cells showed the first maximal response after 39 h. When the responses of CR+ -B cells 27 h and of CR- -B cells 39 h after various pulsed exposures to the mitogen were compared with those after continuous exposures to the mitogen, two successive 3 h exposures (0-3 and 12-15 h for CR+ -B cells; 0-3 and 21-24 h for CR- -B cells) yielded the same level of response as continuous exposure for 27 h (CR+ -B cells) or 39 h (CR- -B cells). These results indicate that both B-cell subpopulations require two signals for maximal stimulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bianco C., Patrick R., Nussenzweig V. A population of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. I. Separation and characterization. J Exp Med. 1970 Oct 1;132(4):702–720. doi: 10.1084/jem.132.4.702. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dukor P., Bianco C., Nussenzweig V. Tissue localization of lymphocytes bearing a membrane receptor for antigen-antibody-complement complexes. Proc Natl Acad Sci U S A. 1970 Oct;67(2):991–997. doi: 10.1073/pnas.67.2.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Galanos C., Lüderitz O., Westphal O. A new method for the extraction of R lipopolysaccharides. Eur J Biochem. 1969 Jun;9(2):245–249. doi: 10.1111/j.1432-1033.1969.tb00601.x. [DOI] [PubMed] [Google Scholar]
  4. Galanos C., Rietschel E. T., Lüderitz O., Westphal O. Interaction of lipopolysaccharides and lipid A with complement. Eur J Biochem. 1971 Mar 1;19(1):143–152. doi: 10.1111/j.1432-1033.1971.tb01298.x. [DOI] [PubMed] [Google Scholar]
  5. Golub E. S. Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells. Cell Immunol. 1971 Aug;2(4):353–361. doi: 10.1016/0008-8749(71)90070-0. [DOI] [PubMed] [Google Scholar]
  6. Gunther G. R., Wang J. L., Edelman G. M. The kinetics of cellular commitment during stimulation of lymphocytes by lectins. J Cell Biol. 1974 Aug;62(2):366–377. doi: 10.1083/jcb.62.2.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hoffmann M. K., Hämmerling U., Simon M., Oettgen H. F. Macrophage requirements of CR- and CR+ B lymphocytes for antibody production in vitro. J Immunol. 1976 May;116(5):1447–1451. [PubMed] [Google Scholar]
  8. Hämmerling U., Chua R., Hoffmann M. K. Polyclonal activation of CR+ and CR- B lymphocytes: the kinetics of initiation of DNA and immunoglobulin synthesis by lipopolysaccharide. J Immunol. 1978 Mar;120(3):750–753. [PubMed] [Google Scholar]
  9. Lindahl-Kiessling K. Mechanism of phytohemagglutinin (PHA) action. V. PHA compared with concanavalin A (Con A). Exp Cell Res. 1972 Jan;70(1):17–26. doi: 10.1016/0014-4827(72)90176-0. [DOI] [PubMed] [Google Scholar]
  10. Ly I. A., Mishell R. I. Separation of mouse spleen cells by passage through columns of sephadex G-10. J Immunol Methods. 1974 Aug;5(3):239–247. doi: 10.1016/0022-1759(74)90108-2. [DOI] [PubMed] [Google Scholar]
  11. Morrison D. C., Jacobs D. M. Binding of polymyxin B to the lipid A portion of bacterial lipopolysaccharides. Immunochemistry. 1976 Oct;13(10):813–818. doi: 10.1016/0019-2791(76)90181-6. [DOI] [PubMed] [Google Scholar]
  12. Novogrodsky A., Katchalski E. Lymphocyte transformation induced by concanavalin A and its reversion by methyl-alpha-D-mannopyranoside. Biochim Biophys Acta. 1971 Jan 28;228(2):579–583. doi: 10.1016/0005-2787(71)90064-5. [DOI] [PubMed] [Google Scholar]
  13. Pauli R. M., Strauss B. S. Proliferation of human peripheral lymphocytes. Characteristics of cells once stimulated or re-stimulated by concanavalin A. Exp Cell Res. 1973 Dec;82(2):357–366. doi: 10.1016/0014-4827(73)90353-4. [DOI] [PubMed] [Google Scholar]
  14. Toyoshima S., Akiyama Y., Nakano K., Tonomura A., Osawa T. A phytomitogen from Wistaria floribunda seeds and its interaction with human peripheral lymphocytes. Biochemistry. 1971 Nov 23;10(24):4457–4463. doi: 10.1021/bi00800a016. [DOI] [PubMed] [Google Scholar]
  15. Toyoshima S., Iwata M., Osawa T. Kinetics of lymphocyte stimulation by concanavalin A. Nature. 1976 Dec 2;264(5585):447–449. doi: 10.1038/264447a0. [DOI] [PubMed] [Google Scholar]
  16. Tse H., Dutton R. W. Separation of helper and suppressor T lymphocytes on a ficoll velocity sedimentation gradient. J Exp Med. 1976 May 1;143(5):1199–1210. doi: 10.1084/jem.143.5.1199. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Weber T. H., Skoog V. T., Mattsson A., Lindahl-Kiessling K. Kinetics of lymphocyte stimulation in vitro by non-specific mitogens. Exp Cell Res. 1974 Apr;85(2):351–361. doi: 10.1016/0014-4827(74)90136-0. [DOI] [PubMed] [Google Scholar]
  18. Yokoyama K., Terao T., Osawa T. Carbohydrate-binding specificity of pokeweed mitogens. Biochim Biophys Acta. 1978 Jan 18;538(2):384–396. doi: 10.1016/0304-4165(78)90366-5. [DOI] [PubMed] [Google Scholar]
  19. Yokoyama K., Terao T., Osawa T. Isolation and characterization of membrane receptors for pokeweed mitogens from mouse lymphocytes. Biochem J. 1977 Sep 1;165(3):431–437. doi: 10.1042/bj1650431. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Yokoyama K., Yano O., Terao T., Osawa T. Purification and biological activities of pokeweek (Phytolacca americana) mitogens. Biochim Biophys Acta. 1976 Apr 14;427(2):443–452. doi: 10.1016/0005-2795(76)90187-2. [DOI] [PubMed] [Google Scholar]
  21. Zoschke D. C., Bach F. H. Specificity of antigen recognition by human lymphocytes in vitro. Science. 1970 Dec 25;170(3965):1404–1406. doi: 10.1126/science.170.3965.1404. [DOI] [PubMed] [Google Scholar]

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