Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1979 Jul;37(3):689–696.

Stimulation of phosphatidylinositol turnover in the macrophage plasma membrane: a possible mechanism for signal transmission

Helga M Ögmundsdóttir, D M Weir
PMCID: PMC1457739  PMID: 315365

Abstract

The effect of various stimuli on the rate of phosphatidylinositol turnover by mouse peritoneal macrophages was studied by measuring the incorporation of myo-[2-3H]-inositol. It was found that the macrophage-activating agents endotoxin and Corynebacterium parvum caused an increase in phosphatidylinositol turnover whilst inert particles (Staphylococcus albus, latex and carbon) had no effect even though they were phagocytosed. The stimulation by C. parvum was dependent on the presence of T cells. T lymphocytes from normal mice and mice immunized with C. parvum were equally effective and it was found that both normal and immune T cells could be stimulated by C. parvum as indicated by an increased uptake of [6-3H]-thymidine. Supernatants from normal or immune spleen cells cultured with and without C. parvum were ineffective and could not replace intact T cells, indicating that cell to cell contact is required or a labile factor that acts over a short range. The time course of stimulation of phosphatidylinositol turnover by macrophages was studied using endotoxin. The rate of turnover increased slowly over a few hours and was still rising at 6 h but decreasing again at 24 h. The increase in bacteriostatic activity against Listeria monocytogenes by macrophages exposed to endotoxin took longer to develop and was more marked at 24 h than at 4 h. The differences between pathways leading to phagocytosis or chemotaxis and those resulting in activation are discussed.

Full text

PDF
689

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Christie G. H., Bomford R. Mechanisms of macrophage activation by Corynebacterium parvum. I. In vitro experiments. Cell Immunol. 1975 May;17(1):141–149. doi: 10.1016/s0008-8749(75)80014-1. [DOI] [PubMed] [Google Scholar]
  2. Christophe J., Frandsen E. K., Conlon T. P., Krishna G., Gardner J. D. The importance of calcium for the regulation of cyclic guanosine 3': 5'-monophosphate levels by pancreozymin, carbamoylcholine and ionophore A-23187 in isolated pancreatic acinar cells. Arch Int Physiol Biochim. 1975 Dec;83(5):951–952. [PubMed] [Google Scholar]
  3. Churchill W. H., Jr, Piessens W. F., Sulis C. A., David J. R. Macrophages activated as suspension cultures with lymphocyte mediators devoid of antigen become cytotoxic for tumor cells. J Immunol. 1975 Sep;115(3):781–786. [PMC free article] [PubMed] [Google Scholar]
  4. Diamantstein T., Ulmer A. Two distinct lumphocyte-stimulating soluble factors (LAF) released from murine peritoneal cells. I. The cellular source and the effect of cGMP on their release. Immunology. 1976 May;30(5):741–747. [PMC free article] [PubMed] [Google Scholar]
  5. Ghaffar A., Cullen R. T., Dunbar N., Woodruff M. F. Anti-tumour effect in vitro of lymphocytes and macrophages from mice treated with Corynebacterium parvum. Br J Cancer. 1974 Mar;29(3):199–205. doi: 10.1038/bjc.1974.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Graham R. C., Jr, Karnovsky M. J., Shafer A. W., Glass E. A., Karnovsky M. L. Metabolic and morphological observations on the effect of surface-active agents of leukocytes. J Cell Biol. 1967 Mar;32(3):629–647. doi: 10.1083/jcb.32.3.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hand W. L., King N. L., Johnson J. D., Lowe D. A. Requirement for magnesium influx in activation of alveolar macrophages mediated by ionophore A23187. Nature. 1977 Feb 10;265(5594):543–544. doi: 10.1038/265543a0. [DOI] [PubMed] [Google Scholar]
  8. Higgins T. J., Winston C. T., David J. R. Effect of migration inhibitory factor (MIF) on cyclic AMP levels of guinea pig macrophages. Cell Immunol. 1976 Nov;27(1):11–16. doi: 10.1016/0008-8749(76)90148-9. [DOI] [PubMed] [Google Scholar]
  9. Maino V. C., Hayman M. J., Crumpton M. J. Relationship between enhanced turnover of phosphatidylinositol and lymphocyte activation by mitogens. Biochem J. 1975 Jan;146(1):247–252. doi: 10.1042/bj1460247. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Michell R. H., Jones L. M., Jafferji S. S. A possible role for phosphatidylinositol breakdown in muscarinic cholinergic stimulus-response coupling. Biochem Soc Trans. 1977;5(1):77–81. doi: 10.1042/bst0050077. [DOI] [PubMed] [Google Scholar]
  11. Pick E. The mechanism of action of soluble lymphocyte mediators. IV. Effect of migratio inhibitory factor (MIF) on macrophage cyclic AMP and on responsiveness to adenylate cyclase stimulators. Cell Immunol. 1977 Aug;32(2):329–339. doi: 10.1016/0008-8749(77)90209-x. [DOI] [PubMed] [Google Scholar]
  12. Wahl L. M., Wahl S. M., Mergenhagen S. E., Martin G. R. Collagenase production by endotoxin-activated macrophages. Proc Natl Acad Sci U S A. 1974 Sep;71(9):3598–3601. doi: 10.1073/pnas.71.9.3598. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Weir D. M., Ogmundsdóttir H. M. Non-specific recognition mechanisms by mononuclear phagocytes. Clin Exp Immunol. 1977 Nov;30(2):323–329. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES