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. 1980 Jan;39(1):109–117.

Limited specific T-cell mediated cytolysis in the absence of extracellular Ca2+.

I C MacLennan, F M Gotch, P Golstein
PMCID: PMC1457778  PMID: 6769782

Abstract

Mouse lymphocytes sensitized either in vitro or in vivo with allogeneic cells were analysed for their cytolytic activity incomplete or Ca2+-deprived medium. The deprived medium was obtained by adding EGTA in excess of the molar Ca2+ concentration but not exceeding that of Ca2+ plus Mg2+. The cytolysis in complete medium was between five and thirty-fold greater in terms of lytic units, than that in Ca2+-deprived medium. This applied equally for effectors sensitized in vivo or in vitro although the overall lytic capacity of the former was greater. The cytolysis in Ca2+-deprived medium was judged to be Mg2+ dependent, as little or no cytolysis was seen if EDTA was added to cultures in a molar concentration exceeding that of Ca2+ plus Mg2+. Direct comparison of cytolysis in complete and deprived medium showed no difference between effectors in the two situations with respect to (a) adherence to nylon wool; (b) sensitivity to lysis by anti-Thy-1.2 antiserum and complement; (c) specificity of lysis; (d) rate of cytolysis with time when effector cell numbers were adjusted to give equal levels of cytolysis at any one time; and (e) shape and angle of the slope of 51Cr release against log increase in effector cell numbers where target cell numbers were constant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Golstein P., Foa C., MacLennan I. C. Mechanism of T cell-mediated cytolysis: the differential impact of cytochalasins at the recognition and lethal hit stages. Eur J Immunol. 1978 May;8(5):302–309. doi: 10.1002/eji.1830080504. [DOI] [PubMed] [Google Scholar]
  2. Golstein P., Gomperts B. D. Non-T cell-mediated cytolysis of antibody-coated sheep red blood cells requires Mg++ but not Ca++: An argument against a conventional "stimulus-secretion" mechanism for cytolysis. J Immunol. 1975 Apr;114(4):1264–1268. [PubMed] [Google Scholar]
  3. Golstein P., Smith E. T. Mechanism of T-cell-mediated cytolysis: the lethal hit stage. Contemp Top Immunobiol. 1977;7:273–300. doi: 10.1007/978-1-4684-3054-7_8. [DOI] [PubMed] [Google Scholar]
  4. Golstein P., Smith E. T. The lethal hit stage of mouse T and non-T cell-mediated cytolysis: differences in cation requirements and characterization of an analytical "cation pulse" method. Eur J Immunol. 1976 Jan;6(1):31–37. doi: 10.1002/eji.1830060108. [DOI] [PubMed] [Google Scholar]
  5. Henney C. S., Bubbers J. E. Studies on the mechanism of lymphocyte-mediated cytolysis. I. The role of divalent cations in cytolysis by T lymphocytes. J Immunol. 1973 Jan;110(1):63–72. [PubMed] [Google Scholar]
  6. Henney C. S. T-Cell-mediated cytolysis: an overview of some current issues. Contemp Top Immunobiol. 1977;7:245–272. doi: 10.1007/978-1-4684-3054-7_7. [DOI] [PubMed] [Google Scholar]
  7. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  8. MacDonald H. R. Early detection of potentially lethal events in T cell-mediated cytolysis. Eur J Immunol. 1975 Apr;5(4):251–254. doi: 10.1002/eji.1830050406. [DOI] [PubMed] [Google Scholar]
  9. MacLennan I. C., Golstein P. Recognition by cytolytic T and K cells: identification in both systems of a divalent cation-independent, cytochalasin A-sensitive step. J Immunol. 1978 Dec;121(6):2542–2546. [PubMed] [Google Scholar]
  10. MacLennan I. C., Golstein P. Requirement for hexose, unrelated to energy provision, in T-cell-mediated cytolysis at the lethal hit stage. J Exp Med. 1978 Jun 1;147(6):1551–1567. doi: 10.1084/jem.147.6.1551. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Martz E., Benacerraf B. An effector-cell independent step in target cell lysis by sensitized mouse lymphocytes. J Immunol. 1973 Nov;111(5):1538–1545. [PubMed] [Google Scholar]
  12. Martz E. Early steps in specific tumor cell lysis by sensitized mouse T lymphocytes. I. Resolution and characterization. J Immunol. 1975 Jul;115(1):261–267. [PubMed] [Google Scholar]
  13. Martz E. Inability of EDTA to prevent damage mediated by cytolytic T-lymphocytes. Cell Immunol. 1975 Dec;20(2):304–314. doi: 10.1016/0008-8749(75)90107-0. [DOI] [PubMed] [Google Scholar]
  14. Martz E. Mechanism of specific tumor-cell lysis by alloimmune T lymphocytes: resolution and characterization of discrete steps in the cellular interaction. Contemp Top Immunobiol. 1977;7:301–361. doi: 10.1007/978-1-4684-3054-7_9. [DOI] [PubMed] [Google Scholar]
  15. Mauel J., Rudolf H., Chapuis B., Brunner K. T. Studies of allograft immunity in mice. II. Mechanism of target cell inactivation in vitro by sensitized lymphocytes. Immunology. 1970 Apr;18(4):517–535. [PMC free article] [PubMed] [Google Scholar]
  16. Miller R. G., Dunkley M. Quantitative analysis of the 51Cr release cytotoxicity assay for cytotoxic lymphocytes. Cell Immunol. 1974 Nov;14(2):284–302. doi: 10.1016/0008-8749(74)90212-3. [DOI] [PubMed] [Google Scholar]
  17. Plaut M., Bubbers J. E., Henney C. S. Studies of the mechanism of lymphocyte-mediated cytolysis. VII. Two stages in the T cell-mediated lytic cycle with distinct cation requirements. J Immunol. 1976 Jan;116(1):150–155. [PubMed] [Google Scholar]
  18. Stulting R. D., Berke G. Nature of lymphocyte-tumor interaction. A general method for cellular immunoabsorption. J Exp Med. 1973 Apr 1;137(4):932–942. doi: 10.1084/jem.137.4.932. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wagner H., Röllinghoff M. T cell-mediated cytotoxicity: discrimination between antigen recognition, lethal hit and cytolysis phase. Eur J Immunol. 1974 Nov;4(11):745–750. doi: 10.1002/eji.1830041108. [DOI] [PubMed] [Google Scholar]

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