Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1980 Jan;39(1):119–125.

Characterization of cell-mediated cytolytic mechanisms against human transitional cell carcinoma line, 253J.

W J Catalona, T L Ratliff, R E McCool
PMCID: PMC1457779  PMID: 7380462

Abstract

We examined the nature of effector cells that mediate antibody-dependent cell-mediated cytotoxicity (ADCC) and spontaneous cell-mediated cytotoxicity (SCMC) against the 253J human bladder transitional cell carcinoma cell line. Fractionation of peripheral blood lymphocytes (PBL) showed that ADCC and SCMC against the 253J targets were mediated by non-adherent, surface immunoglobulin-negative lymphocytes lacking or having only weak affinity for sheep erythrocytes. Macrophages were not cytotoxic against 253J cells in 18 h culture, as removal of macrophages by glass adherence or treatment with silica particles did not reduce ADCC or SCMC, and macrophage-enriched effector cell preparations were only weakly cytotoxic.

Full text

PDF
119

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENNETT B., OLD L. J., BOYSE E. A. THE PHAGOCYTOSIS OF TUMOR CELLS IN VITRO. Transplantation. 1964 Mar;2:183–202. doi: 10.1097/00007890-196403000-00003. [DOI] [PubMed] [Google Scholar]
  2. Broder S., Edelson R. L., Lutzner M. A., Nelson D. L., MacDermott R. P., Durm M. E., Goldman C. K., Meade B. D., Waldmann T. A. The Sézary syndrome: a malignant proliferation of helper T cells. J Clin Invest. 1976 Dec;58(6):1297–1306. doi: 10.1172/JCI108585. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Catalona W. J., Ratliff T. L., McCool R. E. Discordance among cell-mediated cytolytic mechanisms in cancer patients: importance of the assay system. J Immunol. 1979 Mar;122(3):1009–1014. [PubMed] [Google Scholar]
  4. Catalona W. J., Ratliff T. L., McCool R. E. Effects of carrageenan on spontaneous and antibody-dependent cell-mediated cytotoxicity. Cell Immunol. 1978 Sep 15;40(1):1–15. doi: 10.1016/0008-8749(78)90310-6. [DOI] [PubMed] [Google Scholar]
  5. Chess L., MacDermott R. P., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. I. Quantitative isolation of human T and B cells and response to mitogens. J Immunol. 1974 Oct;113(4):1113–1121. [PubMed] [Google Scholar]
  6. Elliott A. Y., Cleveland P., Cervenka J., Castro A. E., Stein N., Hakala T. R., Fraley E. E. Characterization of a cell line from human transitional cell cancer of the urinary tract. J Natl Cancer Inst. 1974 Nov;53(5):1341–1349. doi: 10.1093/jnci/53.5.1341. [DOI] [PubMed] [Google Scholar]
  7. Hibbs J. B., Jr, Taintor R. R., Chapman H. A., Jr, Weinberg J. B. Macrophage tumor killing: influence of the local environment. Science. 1977 Jul 15;197(4300):279–282. doi: 10.1126/science.327547. [DOI] [PubMed] [Google Scholar]
  8. Holtermann O. A., Djerassi I., Lisafeld B. A., Elias E. G., Papermaster B. W., Klein E. In vitro destruction of tumor cells by human monocytes. Proc Soc Exp Biol Med. 1974 Nov;147(2):456–459. doi: 10.3181/00379727-147-38363. [DOI] [PubMed] [Google Scholar]
  9. Horwitz D. A., Lobo P. I. Characterizaiton of two populations of human lymphocytes bearing easily detectable surface immunoglobulin. J Clin Invest. 1975 Dec;56(6):1464–1472. doi: 10.1172/JCI108227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kay H. D., Bonnard G. D., West W. H., Herberman R. B. A functional comparison of human Fc-receptor-bearing lymphocytes active in natural cytotoxicity and antibody-dependent cellular cytotoxicity. J Immunol. 1977 Jun;118(6):2058–2066. [PubMed] [Google Scholar]
  11. Kohl S., Starr S. E., oleske J. M., Shore S. L., Ashman R. B., Nahmias A. J. Human monocyte-macrophage-mediated antibody-dependent cytotoxicity to herpes simplex virus-infected cells. J Immunol. 1977 Mar;118(3):729–735. [PubMed] [Google Scholar]
  12. Mac Dermott R. P., Chess L., Schlossman S. F. Immunologic functions of isolated human lymphocyte subpopulations. V. Isolation and functional analysis of a surface Ig negative, E rosette negative subset. Clin Immunol Immunopathol. 1975 Sep;4(3):415–424. doi: 10.1016/0090-1229(75)90010-0. [DOI] [PubMed] [Google Scholar]
  13. MacDonald H. R., Bonnard G. D., Sordat B., Zawodnik S. A. Antibody-dependent cell-mediated cytotoxicity: heterogeneity of effector cells in human peripheral blood. Scand J Immunol. 1975 Sep;4(5-6):487–497. doi: 10.1111/j.1365-3083.1975.tb02654.x. [DOI] [PubMed] [Google Scholar]
  14. Mantovani A., Caprioli V., Gritti P., Spreafico F. Human mature macrophages mediate antibody-dependent cellular cytotoxicity on tumour cells. Transplantation. 1977 Oct;24(4):291–293. doi: 10.1097/00007890-197710000-00010. [DOI] [PubMed] [Google Scholar]
  15. Mendes N. F., Tolnai M. E., Silveira N. P., Gilbertsen R. B., Metzgar R. S. Technical aspects of the rosette tests used to detect human complement receptor (B) and sheep erythrocyte-binding (T) lymphocytes. J Immunol. 1973 Sep;111(3):860–867. [PubMed] [Google Scholar]
  16. Nelson D. L., Bundy B. M., Pitchon H. E., Blaese R. M., Strober W. The effector cells in human peripheral blood mediating mitogen-induced cellular cytotoxicity and antibody-dependent cellular cytotoxicity. J Immunol. 1976 Nov;117(5 Pt 1):1472–1481. [PubMed] [Google Scholar]
  17. O'Toole C., Saxon A., Bohrer R. Human lymph node lymphocytes fail to effect lysis of antibody-coated target cells. Clin Exp Immunol. 1977 Jan;27(1):165–171. [PMC free article] [PubMed] [Google Scholar]
  18. Pape G. R., Troye M., Perlmann P. Characterization of cytolytic effector cells in peripheral blood of healthy individuals and cancer patients. I. Surface markers and K cell activity after separation of B cells and lymphocytes and Fc-receptors by column fractionation. J Immunol. 1977 Jun;118(6):1919–1924. [PubMed] [Google Scholar]
  19. Peter H. H., Pavie-Fischer J., Fridman W. H., Aubert C., Cesarini J. P., Roubin R., Kourilsky F. M. Cell-mediate cytotoxicity in vitro of human lymphocytes against a tissue culture melanoma cell line (igr3). J Immunol. 1975 Aug;115(2):539–548. [PubMed] [Google Scholar]
  20. Sharma S. D., Piessens W. F. Tumor cell killing by macrophages activated in vitro with lymphocyte mediators. I. Development of a short term in vitro microcytotoxicity assay. Cell Immunol. 1978 Apr;37(1):20–30. doi: 10.1016/0008-8749(78)90170-3. [DOI] [PubMed] [Google Scholar]
  21. Shaw G. M., Levy P. C., Lobuglio A. F. Human monocyte cytotoxicity to tumor cells. I. Antibody-dependent cytotoxicity. J Immunol. 1978 Aug;121(2):573–578. [PubMed] [Google Scholar]
  22. Vessella R. L., Gormus B. J., Lange P. H., Kaplan M. E. Heterogeneity among human lymphocyte effector cells mediating spontaneous lymphocyte-mediated cytotoxicity. Int J Cancer. 1978 May 15;21(5):594–603. doi: 10.1002/ijc.2910210509. [DOI] [PubMed] [Google Scholar]
  23. West W. H., Boozer R. B., Herberman R. B. Low affinity E-rosette formation by the human K cell. J Immunol. 1978 Jan;120(1):90–95. [PubMed] [Google Scholar]
  24. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES