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. 1980 Jan;39(1):67–73.

The establishment of continuous macrophage cell lines from peripheal blood monocytes.

R C Wardley, M J Lawman, F Hamilton
PMCID: PMC1457786  PMID: 6769783

Abstract

Peripheral blood monocytes were established in cultured from ten different animal species. Using a method of repeated medium changes, monolayers of cells developed from seven of these species. These cells had the following characteristics: they were glass and plastic adherent, phagocytic, possessed many vacuoles and pseudopodia, esterase-positive, exhibited complement and Fc receptors and could partake in antigen-specific immune induction of T cells. These characteristics classify the cells as macrophages. The monolayers could be serially passaged and used for virological and immunological assays.

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Selected References

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