Skip to main content
Immunology logoLink to Immunology
. 1980 Mar;39(3):375–384.

Hidden Thy-1 antigen in a subpopulation of mouse bone marrow cells.

M A Ritter, R J Morris, I Goldschneider
PMCID: PMC1457790  PMID: 7002765

Abstract

Immunofluorescence and cytolysis studies using antisera against the xeno- and alloantigenic components of the Thy-1 molecule have shown that in adult mouse bone marrow more cells bear the xeno than the alloantigen. Allo+ cells form a subset of a larger, xeno+ population. Xeno+ allo- 'prothymocytes' can be induced to express the alloantigen in vitro; this could result from the unmasking of cryptic antigen, the presence of which is demonstrated, rather than de novo antigen synthesis. Some xeno+ allo- cells are involved in splenic haemopoiesis, either as a subpopulation of precursors, or as accessory helper cells.

Full text

PDF
375

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acton R. T., Morris R. J., Williams A. F. Estimation of the amount and tissue distribution of rat Thy-1.1 antigen. Eur J Immunol. 1974 Sep;4(9):598–602. doi: 10.1002/eji.1830040904. [DOI] [PubMed] [Google Scholar]
  2. Bach M. A., Bach J. F. Studies on thymus products. VI. The effects of cyclic nucleotides and prostaglandins on rosette-forming cells. Interactions with thymic factor. Eur J Immunol. 1973 Dec;3(12):778–783. doi: 10.1002/eji.1830031208. [DOI] [PubMed] [Google Scholar]
  3. Barclay A. N., Letarte-Muirhead M., Williams A. F. Purification of the Thy-1 molecule from rat brain. Biochem J. 1975 Dec;151(3):699–706. doi: 10.1042/bj1510699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Basch R. S., Goldstein G. Induction of T-cell differentiation in vitro by thymin, a purified polypeptide hormone of the thymus. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1474–1478. doi: 10.1073/pnas.71.4.1474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cohen A., Schlesinger M. Absorption of guinea pig serum with agar. A method for elimination of itscytotoxicity for murine thymus cells. Transplantation. 1970 Jul;10(1):130–132. doi: 10.1097/00007890-197007000-00027. [DOI] [PubMed] [Google Scholar]
  6. Devreotes P. N., Gardner J. M., Fambrough D. M. Kinetics of biosynthesis of acetylcholine receptor and subsequent incorporation into plasma membrane of cultured chick skeletal muscle. Cell. 1977 Mar;10(3):365–373. doi: 10.1016/0092-8674(77)90023-x. [DOI] [PubMed] [Google Scholar]
  7. Douglas T. C. A rat analog of the mouse theta antigen. Transplant Proc. 1973 Mar;5(1):79–82. [PubMed] [Google Scholar]
  8. Doyle D., Baumann H., England B., Friedman E., Hou E., Tweto J. Biogenesis of plasma membrane glycoproteins in hepatoma tissue culture cells. J Biol Chem. 1978 Feb 10;253(3):965–973. [PubMed] [Google Scholar]
  9. Goldschneider I., Gordon L. K., Morris R. J. Demonstration of Thy-1 antigen on pluripotent hemopoietic stem cells in the rat. J Exp Med. 1978 Nov 1;148(5):1351–1366. doi: 10.1084/jem.148.5.1351. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Golub E. S. Brain-associated stem cell antigen: an antigen shared by brain and hemopoietic stem cells. J Exp Med. 1972 Aug 1;136(2):369–374. doi: 10.1084/jem.136.2.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hunt S. V., Mason D. W., Williams A. F. In rat bone marrow Thy-1 antigen is present on cells with membrane immunoglobulin and on precursors of peripheral B lymphocytes. Eur J Immunol. 1977 Nov;7(11):817–823. doi: 10.1002/eji.1830071114. [DOI] [PubMed] [Google Scholar]
  12. Komuro K., Boyse E. A. In-vitro demonstration of thymic hormone in the mouse by conversion of precursor cells into lymphocytes. Lancet. 1973 Apr 7;1(7806):740–743. doi: 10.1016/s0140-6736(73)92127-2. [DOI] [PubMed] [Google Scholar]
  13. Lennon V. A., Unger M., Dulbecco R. Thy-1: a differentiation marker of potential mammary myoepithelial cells in vitro. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6093–6097. doi: 10.1073/pnas.75.12.6093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Milewicz C., Miller H. C., Esselman W. J. Membrane expression of Thy-1,2 and GM1 ganglioside on differentiating T lymphocytes. J Immunol. 1976 Nov;117(5 PT2):1774–1780. [PubMed] [Google Scholar]
  15. Morris R. J., Letarte-Muirhead M., Williams A. F. Analysis in deoxycholate of three antigenic specificities associated with the rat Thy-1 molecule. Eur J Immunol. 1975 Apr;5(4):282–285. doi: 10.1002/eji.1830050413. [DOI] [PubMed] [Google Scholar]
  16. Morris R. J., Williams A. F. Antigens on mouse and rat lymphocytes recognized by rabbit antiserum against rat brain: the quantitative analysis of a xenogeneic antiserum. Eur J Immunol. 1975 Apr;5(4):274–281. doi: 10.1002/eji.1830050412. [DOI] [PubMed] [Google Scholar]
  17. Morris R. J., Williams A. F. Serological and preliminary biochemical characteristics of a T lymphocyte differentiation antigen detected by rabbit antiserum to rat thymocyte membranes. Eur J Immunol. 1977 Jun;7(6):360–366. doi: 10.1002/eji.1830070607. [DOI] [PubMed] [Google Scholar]
  18. Muirhead M. L., Action R. T., Williams A. F. Preliminary characterization of Thy-1.1 and Ag-B antigens from rat tissues solubilized in detergents. Biochem J. 1974 Oct;143(1):51–61. doi: 10.1042/bj1430051. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nelson P., Christian C., Nirenberg M. Synapse formation between clonal neuroblastoma X glioma hybrid cells and striated muscle cells. Proc Natl Acad Sci U S A. 1976 Jan;73(1):123–127. doi: 10.1073/pnas.73.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. REIF A. E., ALLEN J. M. THE AKR THYMIC ANTIGEN AND ITS DISTRIBUTION IN LEUKEMIAS AND NERVOUS TISSUES. J Exp Med. 1964 Sep 1;120:413–433. doi: 10.1084/jem.120.3.413. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Raff M. C. Two distinct populations of peripheral lymphocytes in mice distinguishable by immunofluorescence. Immunology. 1970 Oct;19(4):637–650. [PMC free article] [PubMed] [Google Scholar]
  22. Ritter M. A., Gordon L. K., Goldschneider I. Distribution of identity of Thy-1-bearing cells during ontogeny in rat hemopoietic and lymphoid tissues. J Immunol. 1978 Dec;121(6):2463–2471. [PubMed] [Google Scholar]
  23. Roelants G. E., Loor F., von Boehmer H., Sprent J., Hägg L. B., Mayor K. S., Rydén A. Five types of lymphocytes (Ig-theta-, Ig-theta+weak, Ig-theta+strong, Ig+theta- and Ig+theta+) characterized by double immunofluorescence and electrophoretic mobility. Organ distribution in normal and nude mice. Eur J Immunol. 1975 Feb;5(2):127–131. doi: 10.1002/eji.1830050211. [DOI] [PubMed] [Google Scholar]
  24. Roelants G. E., Mayor K. S., Hägg L. B., Loor F. Immature T lineage lymphocytes in athymic mice. Presence of TL, lifespan and homeostatic regulation. Eur J Immunol. 1976 Feb;6(2):75–81. doi: 10.1002/eji.1830060202. [DOI] [PubMed] [Google Scholar]
  25. Scheid M. P., Goldstein G., Boyse E. A. The generation and regulation of lymphocyte populations: evidence from differentiative induction systems in vitro. J Exp Med. 1978 Jun 1;147(6):1727–1743. doi: 10.1084/jem.147.6.1727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Scheid M. P., Hoffmann M. K., Komuro K., Hämmerling U., Abbott J., Boyse E. A., Cohen G. H., Hooper J. A., Schulof R. S., Goldstein A. L. Differentiation of T cells induced by preparations from thymus and by nonthymic agents. J Exp Med. 1973 Oct 1;138(4):1027–1032. doi: 10.1084/jem.138.4.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Shortman K. The separation of different cell classes from lymphoid organs. II. The purification and analysis of lymphocyte populations by equilibrium density gradient centrifugation. Aust J Exp Biol Med Sci. 1968 Aug;46(4):375–396. doi: 10.1038/icb.1968.32. [DOI] [PubMed] [Google Scholar]
  28. Storrie B., Goldstein G., Boyse E. A., Hammerling U. Differentiation of thymocytes: evidence that induction of the surface phenotype requires transcription and translation. J Immunol. 1976 May;116(5):1358–1362. [PubMed] [Google Scholar]
  29. TILL J. E., McCULLOCH E. A. A direct measurement of the radiation sensitivity of normal mouse bone marrow cells. Radiat Res. 1961 Feb;14:213–222. [PubMed] [Google Scholar]
  30. Thierfelder S. Haemopoietic stem cells of rats but not of mice express Th-1.1 alloantigen. Nature. 1977 Oct 20;269(5630):691–693. doi: 10.1038/269691a0. [DOI] [PubMed] [Google Scholar]
  31. Thomas D. B., Calderon R. A., Blaxland L. J. A new Thy-1 alloantigen as a temporal marker of T lymphocyte differentiation. Nature. 1978 Oct 26;275(5682):711–715. doi: 10.1038/275711a0. [DOI] [PubMed] [Google Scholar]
  32. Trowbridge I. S., Hyman R., Ferson T., Mazauskas C. Expression of Thy-1 glycoprotein on lectin-resistant lymphoma cell lines. Eur J Immunol. 1978 Oct;8(10):716–723. doi: 10.1002/eji.1830081009. [DOI] [PubMed] [Google Scholar]
  33. Tyan M. L. Allogeneic radiation chimeras. Long-term studies. Transplantation. 1975 Apr;19(4):326–334. doi: 10.1097/00007890-197504000-00008. [DOI] [PubMed] [Google Scholar]
  34. Wiktor-Jedrzejczak W., Sharkie S., Ahmed A., Sell K. W., Santos G. W. Theta-sensitive cell and erythropoiesis: identification of a defect in W/Wv anemic mice. Science. 1977 Apr 15;196(4287):313–315. doi: 10.1126/science.322288. [DOI] [PubMed] [Google Scholar]
  35. el-Arini M. O., Osoba D. Differentiation of thymus-derived cells from precursors in mouse bone marrow. J Exp Med. 1973 Mar 1;137(3):821–837. doi: 10.1084/jem.137.3.821. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES