Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1980 Feb;39(2):239–248.

Regulatory T-cell subpopulations in pregnancy. II. Evidence for suppressive activity of the late phase of MLR.

G Chaouat, G A Voisin
PMCID: PMC1457969  PMID: 6445868

Abstract

Adding mitomycin-treated spleen cells from an allopregnant mouse to a material-strain cells v. paternal-strain cells MLR was previously shown to decrease the intensity of the reaction, due to suppressor T cells. In the present study, spleen cells from allogeneically pregnant mice, were added without treatment at day 2 of an MLR of maternal strain responder cells raised against paternal-strain stimulators. It was shown that, while, on 2 day duration culture versus the same stimulator cells these cells are hyper-reactive compared to controls, yet they lead to a MLR of lower intensity than when the same operation was performed with control cells. This suppressive effect is T-cell dependent and use of high dilutions of anti-Thy 1+C suggests that the suppressor cell has a high density of Thy 1 surface antigen. Involvement of these cells in negative regulation of the late phase of the MLR is suggested. MLRs with cells from allopregnant mice as responders were themselves susceptible to this suppression and even more so than cells from virgin mice, indicating a possible physiological role.

Full text

PDF
239

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chaouat G., Voisin G. A., Escalier D., Robert P. Facilitation reaction (enhancing antibodies and suppressor cells) and rejection reaction (sensitized cells) from the mother to the paternal antigens of the conceptus. Clin Exp Immunol. 1979 Jan;35(1):13–24. [PMC free article] [PubMed] [Google Scholar]
  2. Chaouat G., Voisin G. A. Regulatory T cell subpopulations in pregnancy. I. Evidence for suppressive activity of the early phase of MLR. J Immunol. 1979 Apr;122(4):1383–1388. [PubMed] [Google Scholar]
  3. Clark D. A., McDermott M. R. Impairment of host vs graft reaction in pregnant mice. I. Suppression of cytotoxic T cell generation in lymph nodes draining the uterus. J Immunol. 1978 Oct;121(4):1389–1393. [PubMed] [Google Scholar]
  4. Doyle M. V., Parks D. E., Weigle W. O. Specific suppression of the immune response by HGG tolerant spleen cells. I. Parameters affecting the level of suppression. J Immunol. 1976 Jun;116(6):1640–1645. [PubMed] [Google Scholar]
  5. Eardley D. D., Hugenberger J., McVay-Boudreau L., Shen F. W., Gershon R. K., Cantor H. Immunoregulatory circuits among T-cell sets. I. T-helper cells induce other T-cell sets to exert feedback inhibition. J Exp Med. 1978 Apr 1;147(4):1106–1115. doi: 10.1084/jem.147.4.1106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fitch F. W., Engers H. D., Cerottini J. C., Bruner K. T. Generation of cytotoxic T lymphocytes in vitro. VII. Suppressive effect of irradiated MLC cells on CTL response. J Immunol. 1976 Mar;116(3):716–723. [PubMed] [Google Scholar]
  7. Germain R. N., Thèze J., Kapp J. A., Benacerraf B. Antigen-specific T-cell-mediated suppression. I. Induction of L-glutamic acid60-L-alanine30-L-tyrosine10 specific suppressor T cells in vitro requires both antigen-specific T-cell-suppressor factor and antigen. J Exp Med. 1978 Jan 1;147(1):123–136. doi: 10.1084/jem.147.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hamilton M. S., Hellström I. Altered immune responses in pregnant mice. Transplantation. 1977 May;23(5):423–430. doi: 10.1097/00007890-197705000-00007. [DOI] [PubMed] [Google Scholar]
  9. Nadler L. M., Hodes R. J. Regulatory mechanisms in cell-mediated immune responses. II. Comparison of culture-induced and alloantigen-induced suppressor cells in MLR and CML. J Immunol. 1977 May;118(5):1886–1895. [PubMed] [Google Scholar]
  10. Peck A. B., Bach F. H. A miniaturized mouse mixed leukocyte culture in serum-free and mouse serum supplemented media. J Immunol Methods. 1973 Oct;3(2):147–163. doi: 10.1016/0022-1759(73)90030-6. [DOI] [PubMed] [Google Scholar]
  11. Simpson E., Cantor H. Regulation of the immune response by subclasses of T lymphocytes. II. The effect of adult thymectomy upon humoral and cellular responses in mice. Eur J Immunol. 1975 May;5(5):337–343. doi: 10.1002/eji.1830050509. [DOI] [PubMed] [Google Scholar]
  12. Smith R. N., Powell A. E. The adoptive transfer of pregnancy-induced unresponsiveness to male skin grafts with thymus-dependent cells. J Exp Med. 1977 Sep 1;146(3):899–904. doi: 10.1084/jem.146.3.899. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Tse H. Y., Dutton R. W. Separation of helper and suppressor T lymphocytes. II. Ly phenotypes and lack of DNA synthesis requirement for the generation of concanavalin A helper and suppressor cells. J Exp Med. 1977 Sep 1;146(3):747–758. doi: 10.1084/jem.146.3.747. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES