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. 1980 Jul;40(3):377–383.

Suppression and treatment of experimental allergic encephalitis in guinea-pigs with the bovine spinal cord protein (BSCP).

C F MacPherson
PMCID: PMC1458063  PMID: 6159309

Abstract

Experimental allergic encephalitis (EAE) was suppressed in guinea-pigs that had been sensitized with 50 micrograms of bovine myelin basic protein (MyBP) in Freund's complete adjuvant (FCA) by daily injections of 500 micrograms of the bovine spinal cord protein (BSCP) in saline between day 8 (D 8) and D 23 after sensitization. Injections of 500 micrograms of bovine serum albumin in saline, or saline alone, did not suppress disease. Reversal of clinical disease was achieved with doses of 750 micrograms of BSCP in saline when treatment was begun within a day after the first sign of disease was observed. Six injections of 500 micrograms of BSCP in Freund's incomplete adjuvant (FIA) were required to suppress EAE whether BSCP-FIA injections were begun on D 0 or as late as D 8 when T cells sensitized to MyBP had invaded the central nervous system. When administration of BSCP-FIA was withheld until after disease onset, only three or four injections were needed to reverse clinical signs. BSCP appeared to be as effective quantitatively as MyBP in the suppression or treatment of disease. Because there is no cross-reaction between BSCP and MyBP at the antibody level, the present results raise the possibility that the suppressive activity of BSCP may be due to an amino acid sequence in BSCP and MyBP that is recognized only by T cells. When antigens were injected in FIA at 4 day intervals after sensitization, the onset of disease was delayed up to 5 days. Moreover, even bovine gamma-globulin appeared to have suppressive activity when injected three or four times in FIA. The findings suggest that assessments of the suppressive capacity of an antigen may be inaccurate if the antigen is injected in FIA frequently during the interval between sensitization and onset of disease.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALVORD E. C., Jr, SHAW C. M., HRUBY S., KIES M. W. ENCEPHALITOGEN-INDUCED INHIBITION OF EXPERIMENTAL ALLERGIC ENCEPHALOMYELITIS: PREVENTION, SUPPRESSION AND THERAPY. Ann N Y Acad Sci. 1965 Mar 31;122:333–345. doi: 10.1111/j.1749-6632.1965.tb20218.x. [DOI] [PubMed] [Google Scholar]
  2. Deibler G. E., Driscoll B. F., Kies M. W. Immunochemical and biochemical studies demonstrating the identity of a bovine spinal cord protein (SCP) and a basic protein of bovine peripheral myelin (BF). J Neurochem. 1978 Feb;30(2):401–412. doi: 10.1111/j.1471-4159.1978.tb06544.x. [DOI] [PubMed] [Google Scholar]
  3. Deibler G. E., Martenson R. E., Kies M. W. Large scale preparation of myelin basic protein from central nervous tissue of several mammalian species. Prep Biochem. 1972;2(2):139–165. doi: 10.1080/00327487208061467. [DOI] [PubMed] [Google Scholar]
  4. Driscoll B. F., Kies M. W., Alvord E. C., Jr Protection against experimental allergic encephalomyelitis with peptides derived from myelin basic protein: presence of intact encephalitogenic site is essential. J Immunol. 1976 Jul;117(1):110–114. [PubMed] [Google Scholar]
  5. Gonatas N. K., Howard J. C. Inhibition of experimental allergic encephalomyelitis in rats severely depleted of T cells. Science. 1974 Nov 29;186(4166):839–841. doi: 10.1126/science.186.4166.839. [DOI] [PubMed] [Google Scholar]
  6. Hashim G. A., Sharpe R. D., Carvalho E. F., Stevens L. E. Suppression and reversal of allergic encephalomyelitis in guinea pigs with a non-encephalitogenic analogue of the tryptophan region of the myelin basic protein. J Immunol. 1976 Jan;116(1):126–130. [PubMed] [Google Scholar]
  7. Levine S., Hoenig E. M., Kies M. W. Allergic encephalomyelitis: immunologically specific inhibition of cellular passive transfer by encephalitogenic basic proteins. Clin Exp Immunol. 1970 Apr;6(4):503–517. [PMC free article] [PubMed] [Google Scholar]
  8. Lisak R. P., Falk G. A., Heinze R. G., Kies M. W., Alvord E. C., Jr Dissociation of antibody production from disease suppression in the inhibition of allergic encephalomyelitis by myelin basic protein. J Immunol. 1970 Jun;104(6):1435–1446. [PubMed] [Google Scholar]
  9. MacPherson C. F., Armstrong H. Prevention of experimental allergic encephalitis in Lewis rats by rat and bovine spinal cord proteins. Nature. 1977 Mar 31;266(5601):459–461. doi: 10.1038/266459a0. [DOI] [PubMed] [Google Scholar]
  10. MacPherson C. F., Armstrong H., Tan O. Further characterization of the anti-encephalitogenic protein (SCP): isolation from bovine spinal cord and spinal roots. J Immunol. 1976 Jan;116(1):227–231. [PubMed] [Google Scholar]
  11. MacPherson C. F., Yo S. L. Studies on brain antigens. VI. Prevention of experimental allergic encephalomyelitis by a water-soluble spinal cord protein, 1 -SCP. J Immunol. 1973 May;110(5):1371–1375. [PubMed] [Google Scholar]
  12. Macpherson C. F., Armstrong H., Tan O. Prevention of experimental allergic encephalitis in guinea-pigs with spinal cord protein: optimum pretreatment schedules and appraisal of plausible mechanisms. Immunology. 1977 Aug;33(2):161–166. [PMC free article] [PubMed] [Google Scholar]
  13. Ortiz-Ortiz L., Nakamura R. M., Weigle W. O. T cell requirement for experimental allergic encephalomyelitis induction in the rat. J Immunol. 1976 Aug;117(2):576–579. [PubMed] [Google Scholar]
  14. Ortiz-Ortiz L., Weigle W. O. Cellular events in the induction of experimental allergic encephalomyelitis in rats. J Exp Med. 1976 Sep 1;144(3):604–616. doi: 10.1084/jem.144.3.604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Rauch H. C., Einstein E. R., Csejtey J., Davis W. J. Protective action of the encephalitogen and other basic proteins in experimental allergic encephalomyelitis. Immunochemistry. 1968 Nov;5(6):567–575. doi: 10.1016/0019-2791(68)90092-x. [DOI] [PubMed] [Google Scholar]
  16. Spitler L. E., Von Muller C. M., Fudenberg H. H., Eylar E. H. Experimental allergic encephalitis. Dissociation of cellular immunity to brain protein and disease production. J Exp Med. 1972 Jul 1;136(1):156–174. doi: 10.1084/jem.136.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WAKSMAN B. H., ADAMS R. D. A histologic study of the early lesion in experimental allergic encephalomyelitis in the guinea pig and rabbit. Am J Pathol. 1962 Aug;41:135–162. [PMC free article] [PubMed] [Google Scholar]
  18. Webb C., Teitelbaum D., Herz A., Arnon R., Sela M. Molecular requirements involved in suppression of EAE by synthetic basic copolymers of amino acids. Immunochemistry. 1976 Apr;13(4):333–337. doi: 10.1016/0019-2791(76)90344-x. [DOI] [PubMed] [Google Scholar]
  19. Yo S. L., MacPherson C. F. Studies on brain antigens. V. Purification and partial characterization of an anti-encephalitogenic spinal cord protein (SCP). J Immunol. 1972 Nov;109(5):1009–1016. [PubMed] [Google Scholar]

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