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. 1981 Feb;42(2):297–306.

Clearance kinetics of soluble pre-formed immune complexes containing IgM antibodies in normal and decomplemented rabbits.

G D Harkiss, D L Brown
PMCID: PMC1458079  PMID: 7461732

Abstract

The clearance kinetics of homologous soluble pre-formed 125I-BSA/IgM anti-BSA immune complexes (IgM . IC) injected into the blood was studied in the rabbit. IgM . IC close to equivalence were cleared with a greater velocity than those in x 5 or x 10 antigen excess. Clearance of BSA specifically bound to antibody, was measured by the Farr assay and found to exhibit an exponential mode of elimination suggesting a selective removal of large over small lattice complexes or free antigen. The role of C3 in the clearance of IgM . IC was examined by treating rabbits with cobra venom factor (CVF). While more variation was noted in CVF-treated rabbits, no statistically significant differences could be detected between C3-depleted and control animals. It is, therefore, concluded that soluble preformed IgM . IC are cleared from the rabbit circulation by a C3b-independent mechanism. This finding for soluble IC is clearly different from the clearance of IgM-coated red cells from the circulation, which has previously shown to be highly C3b-dependent.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arend W. P., Mannik M. Studies on antigen-antibody complexes. II. Quantification of tissue uptake of soluble complexes in normal and complement-depleted rabbits. J Immunol. 1971 Jul;107(1):63–75. [PubMed] [Google Scholar]
  2. Atkinson J. P., Frank M. M. Studies on the in vivo effects of antibody. Interaction of IgM antibody and complement in the immune clearance and destruction of erythrocytes in man. J Clin Invest. 1974 Aug;54(2):339–348. doi: 10.1172/JCI107769. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brostoff J., Johns P., Stanworth D. R. Complexed IgE in atopy. Lancet. 1977 Oct 8;2(8041):741–742. doi: 10.1016/s0140-6736(77)90240-9. [DOI] [PubMed] [Google Scholar]
  4. Brown D. L., Lachmann P. J., Dacie J. V. The in vivo behaviour of complement-coated red cells: studies in C6-deficient, C3-depleted and normal rabbits. Clin Exp Immunol. 1970 Sep;7(3):401–421. [PMC free article] [PubMed] [Google Scholar]
  5. Cochrane C. G., Koffler D. Immune complex disease in experimental animals and man. Adv Immunol. 1973;16(0):185–264. doi: 10.1016/s0065-2776(08)60298-9. [DOI] [PubMed] [Google Scholar]
  6. Day J. F., Thornburg R. W., Thorpe S. R., Baynes J. W. Carbohydrate-mediated clearance of antibody . antigen complexes from the circulation. The role of high mannose oligosaccharides in the hepatic uptake of IgM . antigen complexes. J Biol Chem. 1980 Mar 25;255(6):2360–2365. [PubMed] [Google Scholar]
  7. Feinstein A., Munn E. A. Conformation of the free and antigen-bound IgM antibody molecules. Nature. 1969 Dec 27;224(5226):1307–1309. doi: 10.1038/2241307a0. [DOI] [PubMed] [Google Scholar]
  8. Haakenstad A. O., Mannik M. The disappearance kinetics of soluble immune complexes prepared with reduced and alkylated antibodies and with intact antibodies in mice. Lab Invest. 1976 Sep;35(3):283–292. [PubMed] [Google Scholar]
  9. Hautanen A., Linder E. C3b binding immune complexes and immunoconglutinins in human sera. Detection by enzyme-linked immunosorbent assay (ELISA). J Immunol Methods. 1979;30(4):367–380. doi: 10.1016/0022-1759(79)90019-x. [DOI] [PubMed] [Google Scholar]
  10. Kijlstra A., van Es L. A., Daha M. R. The role of complement in the binding and degradation of immunoglobulin aggregates by macrophages. J Immunol. 1979 Dec;123(6):2488–2493. [PubMed] [Google Scholar]
  11. Lay W. H., Nussenzweig V. Ca++-dependent binding of antigen-19 S antibody complexes to macrophages. J Immunol. 1969 May;102(5):1172–1178. [PubMed] [Google Scholar]
  12. Levinsky R. J., Soothill J. F. A test for antigen--antibody complexes in human sera using IgM of rabbit antisera to human immunoglobulins. Clin Exp Immunol. 1977 Sep;29(3):428–435. [PMC free article] [PubMed] [Google Scholar]
  13. MATTHEWS C. M. The theory of tracer experiments with 131I-labelled plasma proteins. Phys Med Biol. 1957 Jul;2(1):36–53. doi: 10.1088/0031-9155/2/1/305. [DOI] [PubMed] [Google Scholar]
  14. Mannik M., Arend M. P., Hall A. P., Gilliland B. C. Studies on antigen-antibody complexes. I. Elimination of soluble complexes from rabbit circulation. J Exp Med. 1971 Apr 1;133(4):713–739. doi: 10.1084/jem.133.4.713. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McConahey P. J., Dixon F. J. A method of trace iodination of proteins for immunologic studies. Int Arch Allergy Appl Immunol. 1966;29(2):185–189. doi: 10.1159/000229699. [DOI] [PubMed] [Google Scholar]
  16. Muller-Eberhard H. J., Nilsson U. R., Dalmasso A. P., Polley M. J., Calcott M. A. A molecular concept of immune cytolysis. Arch Pathol. 1966 Sep;82(3):205–217. [PubMed] [Google Scholar]
  17. Nosslin B. Analysis of disappearance time-curves after single injection of labelled proteins. Ciba Found Symp. 1972;9:113–130. doi: 10.1002/9780470719923.ch7. [DOI] [PubMed] [Google Scholar]
  18. Sabiston B. H., Rose J. E. Effect of cold exposure on the metabolism of immunoglobulins in rabbits. J Immunol. 1976 Jan;116(1):106–111. [PubMed] [Google Scholar]
  19. Schreiber A. D., Frank M. M. Role of antibody and complement in the immune clearance and destruction of erythrocytes. I. In vivo effects of IgG and IgM complement-fixing sites. J Clin Invest. 1972 Mar;51(3):575–582. doi: 10.1172/JCI106846. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. TORRIGIANI G., ROITT I. M. THE ENHANCEMENT OF 19S ANTIBODY PRODUCTION BY PARTICULATE ANTIGEN. J Exp Med. 1965 Jul 1;122:181–193. doi: 10.1084/jem.122.1.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wands J. R., Dienstag J. L., Bhan A. K., Feller E. R., Isselbacher K. J. Circulating immune complexes and complement activation in primary biliary cirrhosis. N Engl J Med. 1978 Feb 2;298(5):233–237. doi: 10.1056/NEJM197802022980502. [DOI] [PubMed] [Google Scholar]
  22. van Snick J. L., Masson P. L. The effect of complement on the ingestion of soluble antigen-antibody complexes and IgM aggregates by mouse peritoneal macrophages. J Exp Med. 1978 Oct 1;148(4):903–914. doi: 10.1084/jem.148.4.903. [DOI] [PMC free article] [PubMed] [Google Scholar]

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