Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1981 Feb;42(2):259–266.

Suppression of BCG cell wall induced delayed-type hypersensitivity by BCG pre-treatment. I. Induction of adherent suppressor cells by live BCG injection and their characterization.

K Kato, K I Yamamoto, M Kakinuma, C Ishihara, I Azuma
PMCID: PMC1458084  PMID: 6450731

Abstract

Previous injections of live Bacillus Calmette-Guérin (BCG) in mice produced a suppression of delayed-type hypersensitivity (DTH) induced by oil-treated BCG cell walls (CW). This phenomenon was analysed by the macrophage migration inhibition (MI) test in which peritoneal exudate cells (PEC) from live BCG-injected mice were mixed with PEC from BCG CW-immunized mice, with the result that the former cells suppressed the MI activity in the latter. We considered the Mi test to be a reliable method for demonstrating the existence of suppressor cells induced by the injection of live BCG. Moreover, we found that the adherent cells of PEC possessed a suppressive effect which was retained even after treatment with either anti-mouse Ig or anti-brain associated theta (BA theta) antigen; that the PEC from mice injected with live BCG on at least the 12th day before cell harvesting showed the suppression; and that the suppression operated across the H-2 barrier.

Full text

PDF
259

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Brehmer W., Anacker R. L., Ribi E. Immunogenicity of cell walls from various mycobacteria against airborne tuberculosis in mice. J Bacteriol. 1968 Jun;95(6):2000–2004. doi: 10.1128/jb.95.6.2000-2004.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Broder S., Humphrey R., Durm M., Blackman M., Meade B., Goldman C., Strober W., Waldmann T. Impaired synthesis of polyclonal (non-paraprotein) immunoglobulins by circulating lymphocytes from patients with multiple myeloma Role of suppressor cells. N Engl J Med. 1975 Oct 30;293(18):887–892. doi: 10.1056/NEJM197510302931801. [DOI] [PubMed] [Google Scholar]
  3. Ellner J. J. Suppressor adherent cells in human tuberculosis. J Immunol. 1978 Dec;121(6):2573–2579. [PubMed] [Google Scholar]
  4. Golub E. S. Brain-associated theta antigen: reactivity of rabbit anti-mouse brain with mouse lymphoid cells. Cell Immunol. 1971 Aug;2(4):353–361. doi: 10.1016/0008-8749(71)90070-0. [DOI] [PubMed] [Google Scholar]
  5. Goodwin J. S., Messner R. P., Bankhurst A. D., Peake G. T., Saiki J. H., Williams R. C., Jr Prostaglandin-producing suppressor cells in Hodgkin's disease. N Engl J Med. 1977 Nov 3;297(18):963–968. doi: 10.1056/NEJM197711032971802. [DOI] [PubMed] [Google Scholar]
  6. Kirchner H., Holden H. T., Herberman Splenic suppressor macrophages induced in mice by injection of Corynebacterium parvum. J Immunol. 1975 Nov;115(5):1212–1216. [PubMed] [Google Scholar]
  7. Klimpel G. R., Henney C. S. BCG-induced suppressor cells. I. Demonstration of a macrophage-like suppressor cell that inhibits cytotoxic T cell generation in vitro. J Immunol. 1978 Feb;120(2):563–569. [PubMed] [Google Scholar]
  8. Lagrange P. H., Mackaness G. B. A stable form of delayed-type hypersensitivity. J Exp Med. 1975 Jan 1;141(1):82–96. doi: 10.1084/jem.141.1.82. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lamoureux G., Poisson R. Letter: B.C.G. and immunological anergy. Lancet. 1974 May 18;1(7864):989–990. doi: 10.1016/s0140-6736(74)91296-3. [DOI] [PubMed] [Google Scholar]
  10. Mackaness G. B., Lagrange P. H., Ishibashi T. The modifying effect of BCG on the immunological induction of T cells. J Exp Med. 1974 Jun 1;139(6):1540–1552. doi: 10.1084/jem.139.6.1540. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Markenson J. A., Morgan J. W., Lockshin M. D., Joachim C., Winfield J. B. Responses of fractionated cells from patients with systemic lupus erythematosus and normals to plant mitogen: evidence for a suppressor population of monocytes. Proc Soc Exp Biol Med. 1978 May;158(1):5–9. doi: 10.3181/00379727-158-40127. [DOI] [PubMed] [Google Scholar]
  12. Scott M. T. Biological effects of the adjuvant Corynebacterium parvum. II. Evidence for macrophage-T-cell interaction. Cell Immunol. 1972 Nov;5(3):469–479. doi: 10.1016/0008-8749(72)90073-1. [DOI] [PubMed] [Google Scholar]
  13. Spring S. B., Nisonoff A. Allotypic markers on Fab fragments of mouse immunoglobulins. J Immunol. 1974 Aug;113(2):470–478. [PubMed] [Google Scholar]
  14. Stobo J. D. Immunosuppression in man: suppression by macrophages can be mediated by interactions with regulatory T cells. J Immunol. 1977 Sep;119(3):918–924. [PubMed] [Google Scholar]
  15. Turcotte R., Lafleur L., Labrèche M. Opposite effects of BCG on spleen and lymph node cells: lymphocyte proliferation and immunoglobulin synthesis. Infect Immun. 1978 Sep;21(3):696–704. doi: 10.1128/iai.21.3.696-704.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Twomey J. J., Laughter A. H., Farrow S., Douglass C. C. Hodgkin's disease. An immunodepleting and immunosuppressive disorder. J Clin Invest. 1975 Aug;56(2):467–475. doi: 10.1172/JCI108113. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Yamamoto K., Anacker R. L., Ribi E. Macrophage Migration Inhibition Studies with Cells from Mice Vaccinated with Cell Walls of Mycobacterium bovis BCG: Relationship Between Inhibitory Activity of Lung Cells and Resistance to Airborne Challenge with Mycobacterium tuberculosis H37Rv. Infect Immun. 1970 Jun;1(6):595–599. doi: 10.1128/iai.1.6.595-599.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Yamamoto K., Karinuma M. Genetic control of granuloma response to oil-associated BCG cell wall vaccine in mice. Microbiol Immunol. 1978;22(6):335–348. doi: 10.1111/j.1348-0421.1978.tb00378.x. [DOI] [PubMed] [Google Scholar]
  19. Yamamoto K., Takahashi Y. Macrophage migration inhibition by serum from desensitized animals previously sensitized with tubercle bacilli. Nat New Biol. 1971 Oct 27;233(43):261–263. doi: 10.1038/newbio233261a0. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES