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. 1980 Aug;40(4):673–681.

Chronic inflammatory reactions in the guinea-pig peritoneal cavity induced by continuous local PPD stimulation. Immune status dependency of the accumulation of lymphocytes and eosinophils.

W B van den Berg, T C Haasakker, A C van Maarsseveen, R J Scheper
PMCID: PMC1458090  PMID: 7000691

Abstract

A chronic inflammation model is described which allows the study of the relationship between the level of specific cell-mediated and humoral immunity to the triggering antigen, and the presence of cells or mediators in the inflammatory exudate. In the present study special attention is paid to the participation of lymphocytes and eosinophils. Normal or FCA-pre-immunized guinea-pigs received repeated intraperitoneal injections with PPD for periods up to 21 weeks. In non-pre-immunized animals the inflammation was characterized by a strong accumulation of eosinophils, whereas only a few lymphocytes were present. In contrast, the FCA-pre-immunized guinea-pigs showed a strong lymphocytic accumulation in the absence of eosinophils. Both peritoneal inflammations were shown to remain dependent on the continuous PPD administration. Therefore, the participation of either lymphocytes or eosinophils could be directly correlated to differences in the specific immune status. Both experimental groups developed similar strong anti-PPD antibody responses as assessed by haemagglutination and passive cutaneous anaphylaxis. Only the FCA-pre-immunized guinea-pigs, however, showed strong cell-mediated immunity. The development of CMI to the continuously administered antigen appears to be a prerequisite for the accumulation of lymphocytes, while it seems to prevent antibody-mediated eosinophil accumulation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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