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. 1980 Dec;41(4):903–911.

Factor affecting the in vitro assessment of opsonization: a study of the kinetics of opsonization using the technique of phagocytic chemiluminescence.

A J Williams, M J Hastings, C S Easmon, P J Cole
PMCID: PMC1458310  PMID: 7007217

Abstract

The importance of several factors involved in the investigation of opsonic defects was studied using phagocytic Luminol-dependent chemiluminescence. The range for the opsonization of zymosan and bakers' yeast by serum from healthy individuals was wide and kinetic studies showed comparative differences for different periods of incubation, serum concentrations and particles. Decay in the opsonic activity of serum stored at different temperatures was demonstrated and its clinical implications emphasized. By using techniques to ablate independently the classical and alternative pathways of complement activation, the contribution of these to the opsonization of zymosan, Staphylococcus aureus (NCTC 6571), Pseudomonas aeruginosa and group B streptococcus (NCTC 11080) by normal and hypogammaglobulinaemic serum at a concentration of 7% was assessed. By comparison of the results obtained for different periods of incubation between particle and serum, the need for consideration of this parameter when assessing opsonic activity was shown. The results using the chemiluminescence assay were compared with those using other methods and were found to correlate well.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alper C. A., Abramson N., Johnston R. B., Jr, Jandl J. H., Rosen F. S. Increased susceptibility to infection associated with abnormalities of complement-mediated functions and of the third component of complement (C3). N Engl J Med. 1970 Feb 12;282(7):350–354. doi: 10.1056/nejm197002122820701. [DOI] [PubMed] [Google Scholar]
  2. Buckley R. H. Plasma therapy in immunodeficiency diseases. Am J Dis Child. 1972 Sep;124(3):376–381. doi: 10.1001/archpedi.1972.02110150074014. [DOI] [PubMed] [Google Scholar]
  3. Giebink G. S., Verhoef J., Peterson P. K., Quie P. G. Opsonic requirements for phagocytosis of Streptococcus pneumoniae types VI, XVIII, XXIII, and XXV. Infect Immun. 1977 Nov;18(2):291–297. doi: 10.1128/iai.18.2.291-297.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Martin A., Lachmann P. J., Halbwachs L., Hobart M. J. Haemolytic diffusion plate assays for factors B and D of the alternative pathway of complement activation. Immunochemistry. 1976 Apr;13(4):317–324. doi: 10.1016/0019-2791(76)90341-4. [DOI] [PubMed] [Google Scholar]
  5. Miller M. E., Nilsson U. R. A familial deficiency of the phagocytosis-enhancing activity of serum related to a dysfunction of the fifth component of complement (C5). N Engl J Med. 1970 Feb 12;282(7):354–358. doi: 10.1056/NEJM197002122820702. [DOI] [PubMed] [Google Scholar]
  6. Peterson P. K., Kim Y., Schmeling D., Lindemann M., Verhoef J., Quie P. G. Complement-mediated phagocytosis of Pseudomonas aeruginosa. J Lab Clin Med. 1978 Dec;92(6):883–894. [PubMed] [Google Scholar]
  7. Soothill J. F., Harvey B. A. Defective opsonization. A common immunity deficiency. Arch Dis Child. 1976 Feb;51(2):91–99. doi: 10.1136/adc.51.2.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Stephens C. G., Williams R. C., Jr, Reed W. P. Classical and alternative complement pathway activation by pneumococci. Infect Immun. 1977 Aug;17(2):296–302. doi: 10.1128/iai.17.2.296-302.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Tofte R. W., Peterson P. K., Kim Y., Quie P. G. Influence of serum concentration on opsonization by the classical and alternative complement pathways. Infect Immun. 1980 Feb;27(2):693–696. doi: 10.1128/iai.27.2.693-696.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Verhoef J., Peterson P. K., Quie P. G. Kinetics of staphylococcal opsonization, attachment, ingestion and killing by human polymorphonuclear leukocytes: a quantitative assay using [3H]thymidine labeled bacteria. J Immunol Methods. 1977;14(3-4):303–311. doi: 10.1016/0022-1759(77)90141-7. [DOI] [PubMed] [Google Scholar]
  11. Verhoef J., Peterson P., Kim Y., Sabath L. D., Quie P. G. Opsonic requirements for staphylococcal phagocytosis. Heterogeneity among strains. Immunology. 1977 Aug;33(2):191–197. [PMC free article] [PubMed] [Google Scholar]

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