cell biology. For the article “Regulation of chromosome stability by the histone H2A variant Htz1, the Swr1 chromatin remodeling complex, and the histone acetyltransferase NuA4,” by Nevan J. Krogan, Kristin Baetz, Michael-Christopher Keogh, Nira Datta, Chika Sawa, Trevor C. Y. Kwok, Natalie J. Thompson, Michael G. Davey, Jeff Pootoolal, Timothy R. Hughes, Andrew Emili, Stephen Buratowski, Philip Hieter, and Jack F. Greenblatt, which appeared in issue 37, September 14, 2004, of Proc. Natl. Acad. Sci. USA (101, 13513–13518; first published September 7, 2004; 10.1073/pnas.0405753101), the authors note that in Fig. 3B, the panel referring to “eaf7Δ, 5 μg/ml Benomyl” was identical to “eaf5Δ, 5 μg/ml Benomyl” immediately above. The corrected figure and its legend appear below. This error does not affect the conclusions of the article.
Fig. 3.
Htz1, SWR-C, and the NuA4 complex function to regulate chromosome stability/transmission. (A) SGA analysis (19) using either a transcription-targeted 384-deletion-strain array or genome-wide kinetochore screens (K.B. and V. Measday, unpublished data) identified numerous synthetic genetic interactions between deletions of genes encoding Htz1, SWR-C, or the NuA4 subunits Vid21 and Yng2, and known chromosome stability/transmission factors (see text for details). Genome-wide screens were carried out with four essential kinetochore genes [skp1–3 (42), cep3–1 (43), ctf13–30 (44), and okp1–5 (45)], whereas the nonessential components (mcm21Δ, mcm22Δ, ctf19Δ, bub1Δ, bub3Δ, mad1Δ, and mad2Δ) were present on the targeted miniarray. (B) Effects of the microtubule destabilizing agent benomyl on the growth of wild-type (NJK28), vid21Δ (NJK1042), eaf5Δ (NJK1259), eaf7Δ (NJK1254), yng2Δ (NJK1482), yaf9Δ (NJK1240), htz1Δ (NJK1527), and swr1Δ (NJK1665) strains. Five-fold serial dilutions of strains starting from an OD600 of 0.1 were plated onto yeast extract/peptone/dextrose plates containing 5 or 15 μg/ml benomyl and incubated for 2 days at 30°C.