Abstract
BalI, a type II restriction-modification (R-M) system from the bacterium, Brevibacterium albidum, recognizes the DNA sequence 5'-TGGCCA-3'. We cloned the genes encoding the BalI restriction endonuclease and methyltransferase and expressed them in Escherichia coli. The two genes were aligned tail-to-tail and their termination codons overlapped. BalI restriction endonuclease and methyltransferase comprise 260 and 280 amino acids, respectively, and have molecular weights of 29 043 and 31 999 Da. The amino acid sequence of BalI methyltransferase is similar to that of other m6A MTases, although it has been categorized as a m5C methyltransferase. A high expression system for the BalI restriction endonuclease was constructed in E. coli for the production of large quantities of enzyme.
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- Gelinas R. E., Myers P. A., Weiss G. H., Roberts R. J., Murray K. A specific endonuclease from Brevibacterium albidum. J Mol Biol. 1977 Aug 15;114(3):433–440. doi: 10.1016/0022-2836(77)90260-1. [DOI] [PubMed] [Google Scholar]
- Günthert U., Trautner T. A. DNA methyltransferases of Bacillus subtilis and its bacteriophages. Curr Top Microbiol Immunol. 1984;108:11–22. doi: 10.1007/978-3-642-69370-0_2. [DOI] [PubMed] [Google Scholar]
- Henikoff S. Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene. 1984 Jun;28(3):351–359. doi: 10.1016/0378-1119(84)90153-7. [DOI] [PubMed] [Google Scholar]
- Malumbres M., Gil J. A., Martín J. F. Codon preference in corynebacteria. Gene. 1993 Nov 30;134(1):15–24. doi: 10.1016/0378-1119(93)90169-4. [DOI] [PubMed] [Google Scholar]
- Roberts R. J., Macelis D. REBASE--restriction enzymes and methylases. Nucleic Acids Res. 1994 Sep;22(17):3628–3639. doi: 10.1093/nar/22.17.3628. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Uemori T., Ishino Y., Fujita K., Asada K., Kato I. Cloning of the DNA polymerase gene of Bacillus caldotenax and characterization of the gene product. J Biochem. 1993 Mar;113(3):401–410. doi: 10.1093/oxfordjournals.jbchem.a124058. [DOI] [PubMed] [Google Scholar]
- Watson N. A new revision of the sequence of plasmid pBR322. Gene. 1988 Oct 30;70(2):399–403. doi: 10.1016/0378-1119(88)90212-0. [DOI] [PubMed] [Google Scholar]
- Wilson G. G. Amino acid sequence arrangements of DNA-methyltransferases. Methods Enzymol. 1992;216:259–279. doi: 10.1016/0076-6879(92)16026-g. [DOI] [PubMed] [Google Scholar]
- Wilson G. G., Murray N. E. Restriction and modification systems. Annu Rev Genet. 1991;25:585–627. doi: 10.1146/annurev.ge.25.120191.003101. [DOI] [PubMed] [Google Scholar]