Skip to main content
NCBI home page
Genetics logoLink to Genetics
. 1998 Jan;148(1):349–360. doi: 10.1093/genetics/148.1.349

Genetic mapping of quantitative trait loci affecting susceptibility to Marek's disease virus induced tumors in F2 intercross chickens.

R L Vallejo 1, L D Bacon 1, H C Liu 1, R L Witter 1, M A Groenen 1, J Hillel 1, H H Cheng 1
PMCID: PMC1459797  PMID: 9475745

Abstract

Marek's disease (MD) is a lymphoproliferative disease caused by the MD virus (MDV), which costs the poultry industry nearly $1 billion annually. To identify quantitative trait loci (QTL) affecting MD susceptibility, the inbred lines 6(3) (MD resistant) and 7(2) (MD susceptible) were mated to create more than 300 F2 chickens. The F2 chickens were challenged with MDV JM strain, moderately virulent) at 1 wk of age and assessed for MD susceptibility. The QTL analysis was divided into three stages. In stage 1, 65 DNA markers selected from the chicken genetic maps were typed on the 40 most MD-susceptible and the 40 most MD-resistant F2 chickens, and 21 markers residing near suggestive QTL were revealed by analysis of variance (ANOVA). In stage 2, the suggestive markers plus available flanking markers were typed on 272 F2 chickens, and three suggestive QTL were identified by ANOVA. In stage 3, using the interval mapping program Map Manager and permutation tests, two significant and two suggestive MD QTL were identified on four chromosomal subregions. Three to five loci collected explained between 11 and 23% of the phenotypic MD variation, or 32-68% of the genetic variance. This study constitutes the first report in the domestic chicken on the mapping of non-major histocompatibility complex QTL affecting MD susceptibility.

Full Text

The Full Text of this article is available as a PDF (127.0 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bacon L. D. Influence of the major histocompatibility complex on disease resistance and productivity. Poult Sci. 1987 May;66(5):802–811. doi: 10.3382/ps.0660802. [DOI] [PubMed] [Google Scholar]
  2. Bishop J. M. Cellular oncogenes and retroviruses. Annu Rev Biochem. 1983;52:301–354. doi: 10.1146/annurev.bi.52.070183.001505. [DOI] [PubMed] [Google Scholar]
  3. Cheng H. H., Levin I., Vallejo R. L., Khatib H., Dodgson J. B., Crittenden L. B., Hillel J. Development of a genetic map of the chicken with markers of high utility. Poult Sci. 1995 Nov;74(11):1855–1874. doi: 10.3382/ps.0741855. [DOI] [PubMed] [Google Scholar]
  4. Churchill G. A., Doerge R. W. Empirical threshold values for quantitative trait mapping. Genetics. 1994 Nov;138(3):963–971. doi: 10.1093/genetics/138.3.963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cockerham C. C., Zeng Z. B. Design III with marker loci. Genetics. 1996 Jul;143(3):1437–1456. doi: 10.1093/genetics/143.3.1437. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Cole R. K. Studies on genetic resistance to Marek's disease. Avian Dis. 1968 Feb;12(1):9–28. [PubMed] [Google Scholar]
  7. Cordell H. J., Todd J. A. Multifactorial inheritance in type 1 diabetes. Trends Genet. 1995 Dec;11(12):499–504. doi: 10.1016/s0168-9525(00)89160-x. [DOI] [PubMed] [Google Scholar]
  8. Crittenden Two levels of genetic resistance to lymphoid leukosis. Avian Dis. 1975 Apr-Jun;19(2):281–292. [PubMed] [Google Scholar]
  9. Darvasi A., Soller M. A simple method to calculate resolving power and confidence interval of QTL map location. Behav Genet. 1997 Mar;27(2):125–132. doi: 10.1023/a:1025685324830. [DOI] [PubMed] [Google Scholar]
  10. Friars G. W., Chambers J. R., Kennedy A., Smith A. D. Selection for resistance to Marek's disease in conjunction with other economic traits in chickens. Avian Dis. 1972 Apr;16(1):2–10. [PubMed] [Google Scholar]
  11. Gavora J. S., Spencer J. L. Studies on genetic resistance of chickens to Marek's disease--a review. Comp Immunol Microbiol Infect Dis. 1979;2(2-3):359–371. doi: 10.1016/0147-9571(79)90022-5. [DOI] [PubMed] [Google Scholar]
  12. Haley C. S., Knott S. A. A simple regression method for mapping quantitative trait loci in line crosses using flanking markers. Heredity (Edinb) 1992 Oct;69(4):315–324. doi: 10.1038/hdy.1992.131. [DOI] [PubMed] [Google Scholar]
  13. Hunt H. D., Pharr G. T., Bacon L. D. Molecular analysis reveals MHC class I intra-locus recombination in the chicken. Immunogenetics. 1994;40(5):370–375. doi: 10.1007/BF01246678. [DOI] [PubMed] [Google Scholar]
  14. Lander E. S., Botstein D. Mapping mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics. 1989 Jan;121(1):185–199. doi: 10.1093/genetics/121.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newberg L. A., Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. doi: 10.1016/0888-7543(87)90010-3. [DOI] [PubMed] [Google Scholar]
  16. Lander E., Kruglyak L. Genetic dissection of complex traits: guidelines for interpreting and reporting linkage results. Nat Genet. 1995 Nov;11(3):241–247. doi: 10.1038/ng1195-241. [DOI] [PubMed] [Google Scholar]
  17. Rebai A., Goffinet B., Mangin B. Comparing power of different methods for QTL detection. Biometrics. 1995 Mar;51(1):87–99. [PubMed] [Google Scholar]
  18. Spencer J. L., Gavora J. S., Grunder A. A., Robertson A., Speckmann G. W. Immunization against Marek's disease: influence of strain of chickens, maternal antibody, and type of vaccine. Avian Dis. 1974 Jan-Mar;18(1):33–44. [PubMed] [Google Scholar]
  19. Stuber C. W. Mapping and manipulating quantitative traits in maize. Trends Genet. 1995 Dec;11(12):477–481. doi: 10.1016/s0168-9525(00)89156-8. [DOI] [PubMed] [Google Scholar]
  20. Witter R. L., Hunt H. D. Poultry vaccines of the future. Poult Sci. 1994 Jul;73(7):1087–1093. doi: 10.3382/ps.0731087. [DOI] [PubMed] [Google Scholar]
  21. Witter R. L., Solomon J. J., Burgoyne G. H. Cell culture techniques for primary isolation of Marek's disease-associated herpesvirus. Avian Dis. 1969 Feb;13(1):101–118. [PubMed] [Google Scholar]
  22. Zeng Z. B. Precision mapping of quantitative trait loci. Genetics. 1994 Apr;136(4):1457–1468. doi: 10.1093/genetics/136.4.1457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Zeng Z. B. Theoretical basis for separation of multiple linked gene effects in mapping quantitative trait loci. Proc Natl Acad Sci U S A. 1993 Dec 1;90(23):10972–10976. doi: 10.1073/pnas.90.23.10972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. von Krosigk C. M., McClary C. F., Vielitz E., Zander D. V. Selection for resistance to Marek's disease and its expected effects on other important traits in white leghorn strain crosses. Avian Dis. 1972 Apr;16(1):11–19. [PubMed] [Google Scholar]

Articles from Genetics are provided here courtesy of Oxford University Press

RESOURCES