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. 1996 Jul 1;24(13):2483–2487. doi: 10.1093/nar/24.13.2483

A new class of genome rare cutters.

A G Veselkov 1, V V Demidov 1, P E Nielson 1, M D Frank-Kamenetskii 1
PMCID: PMC145980  PMID: 8692685

Abstract

Although significant efforts have been directed at developing efficient techniques for rare and super rare genome cutting, only limited success has been achieved. Here we propose a new approach to solve this problem. We demonstrate that peptide nucleic acid 'clamps' (bis-PNAs) bind strongly and sequence specifically to short homopyrimidine sites on lambda and yeast genomic DNAs. Such binding efficiently shields methylation/restriction sites which overlap with the bis-PNA binding sites from enzymatic methylation. After removing the bis-PNA, the genomic DNAs are quantitatively cleaved by restriction enzymes into a limited number of pieces of lengths from several hundred kbp to several Mbp. By combining various bis-PNAs with different methylation/restriction enzyme pairs, a huge new class of genome rare cutters can be created. These cutters cover the range of recognition specificities where very few, if any, cutters are now available.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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