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. 1998 Mar;148(3):1225–1232. doi: 10.1093/genetics/148.3.1225

A genetic linkage map of a cichlid fish, the tilapia (Oreochromis niloticus).

T D Kocher 1, W J Lee 1, H Sobolewska 1, D Penman 1, B McAndrew 1
PMCID: PMC1460020  PMID: 9539437

Abstract

We have constructed a genetic map for a tilapia, Oreochromis niloticus, using DNA markers. The segregation of 62 microsatellite and 112 anonymous fragment length polymorphisms (AFLPs) was studied in 41 haploid embryos derived from a single female. We have identified linkages among 162 (93.1%) of these markers. 95% of the microsatellites and 92% of the AFLPs were linked in the final map. The map spans 704 Kosambi cM in 30 linkage groups covering the 22 chromosomes of this species. Twenty-four of these linkage groups contain at least one microsatellite polymorphism. From the number of markers 15 or fewer cM apart, we estimate a total map length of approximately 1000-1200 cM. High levels of interference are observed, consistent with measurements in other fish species. This map is a starting point for the mapping of single loci and quantitative traits in cichlid fishes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Amati P, Meselson M. Localized Negative Interference in Bacteriophage. Genetics. 1965 Mar;51(3):369–379. doi: 10.1093/genetics/51.3.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Franck J. P., Wright J. M. Conservation of a satellite DNA sequence (SATB) in the tilapiine and haplochromine genome (Pisces: Cichlidae). Genome. 1993 Feb;36(1):187–194. doi: 10.1139/g93-025. [DOI] [PubMed] [Google Scholar]
  3. Hulbert S. H., Ilott T. W., Legg E. J., Lincoln S. E., Lander E. S., Michelmore R. W. Genetic analysis of the fungus, Bremia lactucae, using restriction fragment length polymorphisms. Genetics. 1988 Dec;120(4):947–958. doi: 10.1093/genetics/120.4.947. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Johnson S. L., Africa D., Horne S., Postlethwait J. H. Half-tetrad analysis in zebrafish: mapping the ros mutation and the centromere of linkage group I. Genetics. 1995 Apr;139(4):1727–1735. doi: 10.1093/genetics/139.4.1727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Johnson S. L., Gates M. A., Johnson M., Talbot W. S., Horne S., Baik K., Rude S., Wong J. R., Postlethwait J. H. Centromere-linkage analysis and consolidation of the zebrafish genetic map. Genetics. 1996 Apr;142(4):1277–1288. doi: 10.1093/genetics/142.4.1277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kauffman E. J., Gestl E. E., Kim D. J., Walker C., Hite J. M., Yan G., Rogan P. K., Johnson S. L., Cheng K. C. Microsatellite-centromere mapping in the zebrafish (Danio rerio). Genomics. 1995 Nov 20;30(2):337–341. doi: 10.1006/geno.1995.9869. [DOI] [PubMed] [Google Scholar]
  7. Kocher T. D., Conroy J. A., McKaye K. R., Stauffer J. R. Similar morphologies of cichlid fish in Lakes Tanganyika and Malawi are due to convergence. Mol Phylogenet Evol. 1993 Jun;2(2):158–165. doi: 10.1006/mpev.1993.1016. [DOI] [PubMed] [Google Scholar]
  8. Lander E. S., Green P., Abrahamson J., Barlow A., Daly M. J., Lincoln S. E., Newberg L. A., Newburg L. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics. 1987 Oct;1(2):174–181. doi: 10.1016/0888-7543(87)90010-3. [DOI] [PubMed] [Google Scholar]
  9. Lincoln S. E., Lander E. S. Systematic detection of errors in genetic linkage data. Genomics. 1992 Nov;14(3):604–610. doi: 10.1016/s0888-7543(05)80158-2. [DOI] [PubMed] [Google Scholar]
  10. Thorgaard G. H., Allendorf F. W., Knudsen K. L. Gene-Centromere Mapping in Rainbow Trout: High Interference over Long Map Distances. Genetics. 1983 Apr;103(4):771–783. doi: 10.1093/genetics/103.4.771. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Vos P., Hogers R., Bleeker M., Reijans M., van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 1995 Nov 11;23(21):4407–4414. doi: 10.1093/nar/23.21.4407. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Wright J. M. Nucleotide sequence, genomic organization and evolution of a major repetitive DNA family in tilapia (Oreochromis mossambicus/hornorum). Nucleic Acids Res. 1989 Jul 11;17(13):5071–5079. doi: 10.1093/nar/17.13.5071. [DOI] [PMC free article] [PubMed] [Google Scholar]

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